Process optimization of the extraction condition of β‐amylase from brewer's malt and its application in the maltose syrup production

Abstract

β-Amylase is of important biotechnological aid in maltose syrup production. In this study, the extraction condition of β-amylase from brewer's malt and the optimal dosage of β-amylase in maltose syrup production were optimized using response surface methodology and uniform design method. The optimal extraction condition of β-amylase from brewer's malt was composed of 1:17 (g/v) material/liquid ratio, 44°C extraction temperature, pH 6.4 buffer pH, 2.3 H extraction time, and 1.64 g L NaSO dosage with a predicted β-amylase activity of 1,290.99 U g , which was close to the experimental β-amylase activity of 1,230.22 U g . The optimal dosages of β-amylase used in maltose syrup production were 455.67 U g starch and its application in maltose syrup production led to a 68.37% maltose content in maltose syrup, which was 11.2% and 28.9% higher than those using β-amylases from soybean and microbe (P < 0.01). Thus, β-amylase from brewer's malt was beneficial for production of high maltose syrup.

Carbohydrate Binding Modules: Diversity of Domain Architecture in Amylases and Cellulases From Filamentous Microorganisms

“…Furthermore, β-amylases are another important class of exo-acting enzymes producing mainly maltose by cleaving starch molecules at the non-reducing end of α-1,4-glucan chains ( Ray and Nanda, 1996 ). Their industrial application is essentially for maltose-rich syrup production ( Niu et al, 2018 ). Overall, amylolytic enzymes have been largely used in a broad range of industrial applications, such as foods, detergents, pharmaceuticals, and the paper as well as textile industries ( Ray and Nanda, 1996 ; de Souza and de Oliveira Magalhães, 2010 ).…”

Expression, biochemical and structural characterization of high-specific-activity β-amylase from Bacillus aryabhattai GEL-09 for application in starch hydrolysis

“…In plants, β-amylase is strongly associated with fruit development, ripening, seed germination, and abiotic stress response. Plants, such as sweet potato, soybean, and barley, are frequently used as resources of β-amylase production in industrial fields [ 6 , 12 ]. However, plant-sourced β-amylases have drawbacks, including their requirement for large amounts of grains, complex preparation processes, low storage stability, and high production cost, which restrict their further application.…”