Process optimization for simultaneous production of cellulase, xylanase and ligninase by Saccharomyces cerevisiae SCPW 17 under solid state fermentation using Box-Behnken experimental design

Abstract

Multienzyme complex has attracted increased attention in biofuel technology. They offer solutions to effective degradation of complex plant material into fermentable sugars. Microorganisms, especially bacteria and fungi, are well studied for their ability to produce enzymes complex unlike yeast. Yeast strain isolated from mushroom farm was studied for simultaneous production of cellulase, xylanase and ligninase enzymes using lignocellulose waste as substrates. A response surface methodology (RSM) involving Box-Behnken design (BBD) was used to investigate interaction between variables (moisture content, inoculum size, initial pH, incubation time) that affect enzyme production. Crude filtrate was partially purified and characterised. Yeast strain identified as Saccharomyces cerevisiae SCPW 17 was finally studied. Evaluation of lignocellulose waste for enzyme complex production revealed corn cob to be most effective substrate for cellulase, xylanase and ligninase production with enzyme activity of 17.63 ± 1.45 U/gds, 29.35 ± 1.67 U/gds and 150.75 ± 2.01 μmol/min respectively. Time course study showed maximum enzyme complex production was obtained by day 6 with cellulase activity of 12.5 U/gds, xylanase 48.3 U/gds and ligninase 90.8 μmol/min. Using RSM involving BBD, maximum enzyme activity was found to be 19.51 ± 0.32 U/gds, 56.86 ± 0.38 U/gds, 408.17 ± 1.04 μmol/min for cellulaase, xylanase and ligninase respectively. The developed models were highly significant at probability level of P = 0.0001 and multiple correlation co-efficient (R 2 ) was 0.9563 for cellulase, 0.9532 for xylanase and 0.9780 for ligninase. Enzyme complex was stable at varying pH and temperature conditions. Saccharomyces cerevisiae (SCPW 17) studied produced enzyme complex which can be used for bioconversion of biomass to value-added chemicals.

Enzyme Production Potential of Penicillium oxalicum M1816 and Its Application in Ferulic Acid Production

“…For example, Amadi et al investigated the effects of the fermentation moisture ratio, pH, inoculum size, and incubation time on the cellulase and xylanase activities from Saccharomyces cerevisiae SCPW 17. After the optimization process, the cellulase and xylanase activities increased by 10.66% and 93.73%, respectively [67]. After the fermentation condition optimization, feruloyl esterase (BpFae) activities from Burkholderia pyrrocinia B1213 were increased 2.92 times higher than that obtained under optimal conditions using IPTG as the inducer [68].…”

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

“…Most fungi have the complete enzymatic system (Endoglucanases, Cellobiohydrolases, B-glucosidases, and Xylanases) to degrade this complex cellulosic material for nutrition [123]. Trichoderma reesei is widely applied for the commercial production of cellulases [48], but other fungi also represent potent cellulase producers, including Aspergillus niger [124], Saccharomyces cerevisiae [125], and Aspergillus brasiliensis [126]. Xylan, a complex polysaccharide, is also a major component of hemicellulose; hence, xylanases play an important role in the efficient hydrolysis of plant cellulolytic material [127].…”

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“…Yeasts, like C. tropicalis that can produce extracellular enzymes, have specific genes to express particular enzymes (Yan et al, 2005). The ability of these genes to express enzymes in turn are impacted by their environment as well as the nutrients present there (Amadi et al, 2020). Hence, understanding and proper optimization of the factors affecting the growth of yeast to achieve maximum biomass and enzyme production in culture is necessary.…”