Process optimization and scale-up for production of rabies vaccine live adenovirus vector (AdRG1.3)

Shen, Chun Fang; Lanthier, Stéphane; Jacob, Danielle; Montes, Johnny; Beath, Alex; Beresford, Andrew; Kamen, Amine

doi:10.1016/j.vaccine.2011.10.095

Cited by 32 publications

(25 citation statements)

References 24 publications

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“…Medium composition can greatly influence viral productivity of a cell line [78][79][80]. Additionally, an industrial application of cell cultures requires use of serum-free media, further limiting viral productivity.…”

Section: Culture and Production Mediamentioning

confidence: 99%

“…The efficiency of VLP productions was highly dependent on the medium composition with only two out of five supporting a detectable production. On the other hand, Shen et al have been developing for several years an in-house medium that can sustain both HEK293 cell growth and production of variety of viral vectors with most success in production of adenoviruses [80,81].…”

Section: Culture and Production Mediamentioning

confidence: 99%

“…First, although serum-free media are desired in viral production, addition of foetal bovine serum (FBS) or calf serum (CS) is a well-known strategy to increase viral yields [66,78,80,84]. It Table 2 Examples of viral production performed in HEK293 suspension cells.…”

Section: Fed-batch Feedingmentioning

confidence: 99%

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Influence of HEK293 metabolism on the production of viral vectors and vaccine

Petiot

Čuperlović-Culf

Shen

et al. 2015

Vaccine

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Section: Culture and Production Mediamentioning

confidence: 99%

Section: Culture and Production Mediamentioning

confidence: 99%

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Influence of HEK293 metabolism on the production of viral vectors and vaccine

Petiot

Čuperlović-Culf

Shen

et al. 2015

Vaccine

Self Cite

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“…It has been shown that vaccines based on adenovirus vectors induced an effi cient immune defense against the rabies virus in both dogs [30] and newborn mice when administered by either nasal or oral routes [31]. Rabies vaccines based on different adenovirus strains are already applied for animal vaccination [32]. Some of them are currently undergoing wide scale trials in Canada and the United States as bait preparations for oral vaccination of wild skunks and raccoons [33,34].…”

Section: Rabies Vaccines Based On Viral Vectorsmentioning

confidence: 99%

Rabies vaccines: Current status and prospects for development

et al. 2015

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Rabies is an infectious disease among humans and animals that remains incurable, despite its longstanding research history. The only way to prevent the disease is prompt treatment, including vaccination as an obligatory component and administration of antirabies immunoglobulin as a supplement. Since the first antirabies vaccination performed in the 19th century, a large number of different rabies vaccines have been developed. Progress in molecular biology and biotechnology enabled the development of effective and safe technologies of vaccine production. Currently, new generation vaccines are being developed based on recombinant rabies virus strains or on the production of an individual recombinant rabies antigen-glyco protein (G protein), either as a component of nonpathogenic viruses, or in plants, or in the form of DNA vac cines. In this review, the main modern trends in the development of rabies vaccines have been discussed.

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“…Taking the process developed initially in the milliliter or liter scale and scaling it up to industrial production volumes of hundreds or thousands of liters is a challenging procedure. Maintaining the productivity of the small scale process as well as critical process and product quality attributes are the essential goals of the scale up procedure [116][117][118].…”

Section: Process Scale-up and Implications For Processes Utilizing Thmentioning

confidence: 99%