Process optimization and bioactive compounds quantification from Dactylorhiza hatagirea tuber for alleviating glycemic and oxidative stress

Singh, Laxman; Singh, Bisu; Kewlani, Pushpa; Belwal, Tarun; Bhatt, Indra D.; Nandi, Shyamal K.; Bisht, Anil Kumar

doi:10.1016/j.jarmap.2021.100352

Cited by 15 publications

(12 citation statements)

References 53 publications

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“…This study provides a reference. In a study conducted with Dactylorhiza hatagirea tuber extract, ABTS •+ scavenging activity was found to be 1.06 ± 0.008 mM/g [ 4 ]. In another study, ethanol extracts of Dactylorhiza romana roots were investigated and the ABTS test IC 50 was found to be 0.64 ± 0.005 mg/mL [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

“…Owing to their strong free radical scavenging properties, flavonoids show antioxidant activities such as metal chelation and reduction [ 38 ]. In another study, total phenolic and flavonoid quantities in Dactylorhiza hatagirea tuber extract were found to be 11.42 ± 0.48 mg GAE/g and 11.46 ± 0.28 mg QE/g, respectively [ 4 ]. Dactylorhiza chuhensis ethanol extracts were examined in a study and the total phenolics of tubers and flowers were found to be 13.9 ± 0.6 and 44.2 ± 2.0 mg GAE/g DW, respectively [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

“…The vanilla flavor obtained from orchids is frequently traded [ 2 ]. Orchid species are used for treatment of Alzheimer’s and Parkinson’s diseases (AD and PD), anxiety, depression, cancer, chest pain, tuberculosis, intestinal disorders, dysentery, diarrhea, cough, cold, anemia, and are also used as an aphrodisiac in adults [ 2 , 3 , 4 ]. It has been reported that these medicinal properties are due to secondary compounds such as polyphenolic compounds, ascorbic acid, indole alkaloids, and saponins [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Orchid species are used for treatment of Alzheimer’s and Parkinson’s diseases (AD and PD), anxiety, depression, cancer, chest pain, tuberculosis, intestinal disorders, dysentery, diarrhea, cough, cold, anemia, and are also used as an aphrodisiac in adults [ 2 , 3 , 4 ]. It has been reported that these medicinal properties are due to secondary compounds such as polyphenolic compounds, ascorbic acid, indole alkaloids, and saponins [ 4 ]. It has also been determined that orchid roots have many compounds such as polyphenols and glucomannan, which have strong antioxidant properties [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Glucomannan polysaccharide, which is composed of mannose and glucose, is the main component in orchid flour [ 2 ]. In addition to hot drinks, sahlep is widely used in bakery foods, as an additive for ice cream, and in confectionery and pharmaceuticals [ 4 ]. In Turkey, sahlep is prepared from approximately 120 orchid taxa representing genera such as Dactylorhiza, Orchis, and Ophrys [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Sahlep (Dactylorhiza osmanica): Phytochemical Analyses by LC-HRMS, Molecular Docking, Antioxidant Activity, and Enzyme Inhibition Profiles

et al. 2022

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Studies have shown an inverse correlation among age-related illnesses like coronary heart disease and cancer and intake of fruit and vegetable. Given the probable health benefits of natural antioxidants from plants, research on them has increased. Dactylorhiza osmanica is consumed as a food and traditional medicine plant in some regions of Turkey, so evaluation of the biological ability of this species is important. In this study, the amount of phenolic content (LC-HRMS), antioxidant activities and enzyme inhibitory properties of an endemic plant, D. osmanica, were investigated. The antioxidant capacities of an ethanol extract of D. osmanica aerial parts (EDOA) and roots (EDOR) were evaluated with various antioxidant methods. Additionally, the enzyme inhibitory effects of EDOA and EDOR were examined against acetylcholinesterase (AChE), α-glycosidase, and α-amylase enzymes, which are associated with common and global Alzheimer’s disease and diabetes mellitus. The IC50 values of EDOA against the enzymes were found to be 1.809, 1.098, and 0.726 mg/mL, respectively; and the IC50 values of EDOR against the enzymes were found to be 2.466, 0.442, and 0.415 mg/mL, respectively. Additionally, LC-HRMS analyses revealed p-Coumaric acid as the most plentiful phenolic in both EDOA (541.49 mg/g) and EDOR (559.22 mg/g). Furthermore, the molecular docking interaction of p-coumaric acid, quercitrin, and vanillic acid, which are the most plentiful phenolic compounds in the extracts, with AChE, α-glucosidase, and α-amylase, were evaluated using AutoDock Vina software. The rich phenolic content and the effective antioxidant ability and enzyme inhibition potentials of EDOA and EDOR may support the plant’s widespread food and traditional medicinal uses.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%