Process engineering aspects of the production of cephalosporin C by Acremonium chrysogenum. Part I. Application of complex media

Seidel, G.; Tollnick, C.; Beyer, Michael; Fahimi, Y; Schügerl, K.

doi:10.1016/s0032-9592(02)00081-x

Cited by 16 publications

(9 citation statements)

References 28 publications

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“…To obtain the optimum composition of the cultivation medium it was varied over a broad range. The highest CPC concentrations were obtained by valine, alanine and serine supplements [34,35].…”

Section: Eng Lifementioning

confidence: 94%

“…The highest CPC concentrations were obtained by valine, alanine and serine supplements [34,35]. To obtain the optimum composition of the cultivation medium it was varied over a broad range.…”

Section: Eng Lifementioning

confidence: 99%

“…The use of solid peanut flour resulted in similar problems to those discussed above for the production of xylanase by Aspergillus awamori. Only the production of CPC by the Acremonium chysogenum 3/2(C3) strain from Hoechst Marion Roussel (HMR) is presented, which was cultivated on dry glucose syrup (DGS) and soy oil [29,30]. DGS is a partly hydrolyzed starch with a complex composition of glucose, maltose, oligo-and polysaccharides.…”

Section: Eng Lifementioning

confidence: 99%

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Process Development in Biotechnology - A Re-Evaluation

Schügerl¹

2005

Eng. Life Sci.

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This review considers some process development problems in biotechnology and presents examples of solutions, which were developed in cooperation with industrial partners. These processes include the production of restriction endonuclease EcoRI by recombinant Escherichia coli, which is toxic to the cell, penicillin V by Penicillium chrysogenum, xylanase by Aspergillus awamori, cephalosporin C by Acremonium chrysogenum, erythritol by Moniliella tomentosa var pollinis, and alkaline serine protease by Bacillus licheniformis. Special attention is given to the practical aspects of product development.

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Section: Eng Lifementioning

confidence: 94%

Section: Eng Lifementioning

confidence: 99%

Section: Eng Lifementioning

confidence: 99%

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Process Development in Biotechnology - A Re-Evaluation

Schügerl¹

2005

Eng. Life Sci.

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“…Optimum concentration of methionine stimulates fungal differentiation and increases the antibiotic yield. Hence it is reported that 0.4% of methionine concentration is optimum for the synthesis reaction [15].…”

Section: Factors Effecting Biosynthesis Of Cephalosporin Cmentioning

confidence: 99%

Cephalosporin C Production from Acremonium chrysogenum

Khan¹

2017

Enz Eng

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Cephalosporin C demand is increasing worldwide because of its enhanced antibacterial spectrum that it can be used to treat diseases and infections caused by Gram positive/or Gram negative bacterial strains. Cephalosporin C is easily produced from the fungus Acremonium chrysogenum by the process of fermentation in a bioreactor under optimum reaction conditions to obtain maximum yield of the antibiotic. Additional chemical and structural modifications in the existing cephalosporin C could further enhance its antimicrobial spectrum.

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“…Distinguished from the transsulfuration and direct sulfdrylation for cysteine biosynthesis in P. chrysogenum, reverse transsulfuration pathway and sulfate assimilation were the main biosynthetic pathways in A. chrysogenum [11] and were contained in the stoichiometric model (reactions 32-34). The pathway of CPC biosynthesis started with the condensation of three amino acids of valine, cysteine, and a-AAA to form the tripeptide of a-aminoadipyl-cysteinyl-valine (ACV) [22]. The biosynthesis of the final product CPC (reaction 35) was accomplished by the conversion of ACV to isopenicillin N, epimerization of isopenicillin N, enzymatic expansion/ hydroxylation, and transfer of an acetyl moiety from acetyl coenzyme A successively.…”

Section: Construction Of Metabolic Network In a Chrysogenummentioning

confidence: 99%

Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil

Yang

Chu

et al. 2010

Bioprocess Biosyst Eng

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A metabolic network was constructed for the Acremonium chrysogenum cultivation fed with soybean oil. Metabolic flux analysis indicated that the shift from exponential growth to rapid cephalosporin C (CPC) formation was accompanied by 1.63- and 5-fold carbon flux enlargement in TCA cycle and glyoxylate by-pass, respectively. The flux via pentose phosphate pathway branch was little affected during the rapid CPC formation period; the contributory explanation was that 35.6% of NADPH was consumed in the dissimilation of fatty acids. Estimation of NADPH, ATP generation, and consumption demonstrated that, with soybean oil as carbon source in rapid CPC formation phase, the NADPH consumed in fatty acid catabolism was fourfold greater than that used in the CPC biosynthesis-relevant part; simultaneously, more than 90% energy spent was not directly related to the CPC formation. Therefore, the improvement of CPC production yield through optimization of the NADPH, ATP generation, and consumption was put forward.

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Process engineering aspects of the production of cephalosporin C by Acremonium chrysogenum. Part I. Application of complex media

Cited by 16 publications

References 28 publications

Process Development in Biotechnology - A Re-Evaluation

Process Development in Biotechnology - A Re-Evaluation

Cephalosporin C Production from Acremonium chrysogenum

Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil

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