Process development and optimization of fed-batch production processes for therapeutic proteins by CHO cells

Clincke, Marie-Françoise; Harmsen, Mareike; Malphettes, Laetitia

doi:10.1186/1753-6561-7-s6-p79

Cited by 2 publications

(2 citation statements)

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“…3D suspension culturing is widely used to scale up the production of cells and proteins [ 2 , 12 , 25 , 26 ]. Whether L-Wnt-3a-cells can be cultured in suspension is unknown and has not been studied.…”

Section: Resultsmentioning

confidence: 99%

A simple and scalable hydrogel-based system for culturing protein-producing cells

Wang

et al. 2018

PLoS ONE

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Recombinant protein therapeutics have become important components of the modern medicine. Majority of them are produced with mammalian cells that are cultured either through adherent culturing, in which cells are cultured on substrates, or suspension culturing, in which cells are suspended and cultured in agitated cell culture medium in a culture vessel. The adherent cell culturing method is limited by its low yield. In suspension culturing, cells need extensive genetic manipulation to grow as single cells at high density, which is time- and labor-consuming. Here, we report a new method, which utilizes a thermoreversible hydrogel as the scaffold for culturing protein-expressing cells. The hydrogel scaffolds not only provide 3D spaces for the cells, but also act as physical barriers to prevent excessive cellular agglomeration and protect cells from the hydrodynamic stresses. As a result, cells can grow at high viability, high growth rate, and extremely high yield even without genetic manipulations. The cell yield in the hydrogels is around 20 times of the suspension culturing. In addition, the protein productivity per cell per day in the hydrogel is higher than the adherent culturing method. This new method is simple, scalable and defined. It will be of great value for both the research laboratories and pharmaceutical industry for producing proteins.

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Section: Resultsmentioning

confidence: 99%

A simple and scalable hydrogel-based system for culturing protein-producing cells

Wang

et al. 2018

PLoS ONE

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“…Solche milden Prozessbedingungen werden häufig bei Perfusionsprozessen angewendet, wenn scherempfindliche Säugerzellen in kontinuierlichen Fermentationen im Reaktor zurückgehalten werden sollen, während das extrazellulär produzierte Protein im Permeat kontinuierlich geerntet wird. Bei aktuellen Perfusionsprozessen ist Fouling aufgrund der geringen Überströmungsraten ein beträchtlicher Nachteil in Bezug auf Prozessrobustheit und Produktausbeute 12 und könnte durch Anwendung unsteter Strömungsbedingungen deutlich verringert werden 13, 14.…”

Section: Ergebnisse Der Filtrationsversucheunclassified

Unsteady Crossflow during Microfiltration and Its Impact on Fouling Occurrence

Weinberger

2023

Chemie Ingenieur Technik

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Der Einsatz und die Wirtschaftlichkeit der Crossflow‐Mikrofiltration werden insbesondere durch das Auftreten von Fouling beeinträchtigt. Eine unstete Überströmung, beispielsweise durch eine pulsierende oder alternierende Betriebsweise, können die Bildung einer Deckschicht verringern und damit einen starken Fluxabfall und den Rückhalt von Gelöststoffen verhindern. Dadurch können insbesondere Prozesse mit geringer Überströmgeschwindigkeit oder hohem Transmembrandruck deutlich verbessert werden. Das Ausmaß der Verbesserung hängt dabei stark von der Foulingneigung des zu filtrierenden Fluids ab.

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Process development and optimization of fed-batch production processes for therapeutic proteins by CHO cells

Cited by 2 publications

References 0 publications

A simple and scalable hydrogel-based system for culturing protein-producing cells

A simple and scalable hydrogel-based system for culturing protein-producing cells

Unsteady Crossflow during Microfiltration and Its Impact on Fouling Occurrence

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