Proceedings: Changes in the sterol content of sheep ovarian follicles in culture: relationship to steroidogenesis and effects of gonadotrophins

Seamark, R.F.; Rp, Hamilton; Rm, Moor

doi:10.1530/jrf.0.0460521-a

Cited by 3 publications

(2 citation statements)

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“…On the one hand, the strategies applied to oocytes derived from antral follicles include (1) setting up three-dimensional (3D) cultural systems [ 10 , 11 , 12 , 13 , 14 ], (2) testing the optimal duration of the in vitro culture [ 15 , 16 ], (3) IVM supplementation of growth factors (GFs) and molecules [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], (4) co-cultural supportive incubation with different reproductive-derived cells or stem cells [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ], and (5) development of enclosed-follicle oocyte protocols [ 44 , 45 , 46 , 47 , 48 , 49 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this context, the adoption of a more complex follicle-enclosed oocyte maturation (FEO) method might be promising if applied to early-stage follicles. FEO is an old IVM protocol adopted, firstly, to study the mechanism controlling meiotic quiescence and to reproduce the induction of meiosis (hamster [ 44 ], rat [ 45 ], rabbit [ 46 ], sheep [ 47 , 48 ], porcine [ 49 ]) physiologically. A strategy that initiates with the hormonal triggers and then propagates throughout the somatic compartment of the follicle by the EGF-like peptides (amphiregulin, epiregulin, and betacellulin) has recently been re-proposed to better mimic and synchronize the LH-inducing of oocyte maturation mechanisms [ 61 ].…”

Section: Introductionmentioning

confidence: 99%

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IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Peserico

Berardino

Capacchietti

et al. 2023

IJMS

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In vitro maturation (IVM) is not a routine assisted reproductive technology (ART) for oocytes collected from early antral (EA) follicles, a large source of potentially available gametes. Despite substantial improvements in IVM in the past decade, the outcomes remain low for EA-derived oocytes due to their reduced developmental competences. To optimize IVM for ovine EA-derived oocytes, a three-dimensional (3D) scaffold-mediated follicle-enclosed oocytes (FEO) system was compared with a validated cumulus-oocyte complex (COC) protocol. Gonadotropin stimulation (eCG and/or hCG) and/or somatic cell coculture (ovarian vs. extraovarian-cell source) were supplied to both systems. The maturation rate and parthenogenetic activation were significantly improved by combining hCG stimulation with ovarian surface epithelium (OSE) cells coculture exclusively on the FEO system. Based on the data, the paracrine factors released specifically from OSE enhanced the hCG-triggering of oocyte maturation mechanisms by acting through the mural compartment (positive effect on FEO and not on COC) by stimulating the EGFR signaling. Overall, the FEO system performed on a developed reproductive scaffold proved feasible and reliable in promoting a synergic cytoplasmatic and nuclear maturation, offering a novel cultural strategy to widen the availability of mature gametes for ART.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Peserico

Berardino

Capacchietti

et al. 2023

IJMS

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Changes in the Graafian Follicle Population During the Follicular Phase of the Oestrous Cycle

Hay

Moor

1978

Control of Ovulation

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Bioengineered 3D ovarian model for long-term multiple development of preantral follicle: bridging the gap for poly(ε-caprolactone) (PCL)-based scaffold reproductive applications

Di Berardino,

Peserico,

Camerano Spelta Rapini

et al. 2024

Reprod Biol Endocrinol

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Background Assisted Reproductive Technologies (ARTs) have been validated in human and animal to solve reproductive problems such as infertility, aging, genetic selection/amplification and diseases. The persistent gap in ART biomedical applications lies in recapitulating the early stage of ovarian folliculogenesis, thus providing protocols to drive the large reserve of immature follicles towards the gonadotropin-dependent phase. Tissue engineering is becoming a concrete solution to potentially recapitulate ovarian structure, mostly relying on the use of autologous early follicles on natural or synthetic scaffolds. Based on these premises, the present study has been designed to validate the use of the ovarian bioinspired patterned electrospun fibrous scaffolds fabricated with poly(εcaprolactone) (PCL) for multiple preantral (PA) follicle development.Methods PA follicles isolated from lamb ovaries were cultured on PCL scaffold adopting a validated single-follicle protocol (Ctrl) or simulating a multiple-follicle condition by reproducing an artificial ovary engrafted with 5 or 10 PA (AO 5PA and AO 10PA ). The incubations were protracted for 14 and 18 days before assessing scaffold-based microenvironment suitability to assist in vitro folliculogenesis (ivF) and oogenesis at morphological and functional level. ResultsThe ivF outcomes demonstrated that PCL-scaffolds generate an appropriate biomimetic ovarian microenvironment supporting the transition of multiple PA follicles towards early antral (EA) stage by supporting follicle growth and steroidogenic activation. PCL-multiple bioengineering ivF (AO 10PA ) performed in long term generated, in addition, the greatest percentage of highly specialized gametes by enhancing meiotic competence, large chromatin remodeling and parthenogenetic developmental competence.

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Proceedings: Changes in the sterol content of sheep ovarian follicles in culture: relationship to steroidogenesis and effects of gonadotrophins

Cited by 3 publications

References 0 publications

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Changes in the Graafian Follicle Population During the Follicular Phase of the Oestrous Cycle

Bioengineered 3D ovarian model for long-term multiple development of preantral follicle: bridging the gap for poly(ε-caprolactone) (PCL)-based scaffold reproductive applications

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