IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Peserico, Alessia; Berardino, Chiara Di; Capacchietti, Giulia; Rapini, Chiara Camerano Spelta; Liverani, Liliana; Boccaccını, Aldo R.; Russo, Valentina; Mauro, Annunziata; Barboni, Barbara

doi:10.3390/ijms24076626

Cited by 3 publications

(2 citation statements)

References 130 publications

(173 reference statements)

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“…This system was compared with a conventional cumulus-oocyte complex (COC) protocol. The 3D scaffold promoted synergic cytoplasmatic and nuclear maturation, offering a novel culture strategy to widen the availability of mature gametes for ART ( Peserico et al, 2023 ).…”

Section: D Culture Applied To the Female Reproductive Systemmentioning

confidence: 99%

3D culture applied to reproduction in females: possibilities and perspectives

Ferronato,

Vit,

Silveira

2024

Anim. Reprod.

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In vitro cell culture is a well-established technique present in numerous laboratories in diverse areas. In reproduction, gametes, embryos, and reproductive tissues, such as the ovary and endometrium, can be cultured. These cultures are essential for embryo development studies, understanding signaling pathways, developing drugs for reproductive diseases, and in vitro embryo production (IVP). Although many culture systems are successful, they still have limitations to overcome. Three-dimensional (3D) culture systems can be close to physiological conditions, allowing greater interaction between cells and cells with the surrounding environment, maintenance of the cells' natural morphology, and expression of genes and proteins such as in vivo . Additionally, three-dimensional culture systems can stimulated extracellular matrix generating responses due to the mechanical force produced. Different techniques can be used to perform 3D culture systems, such as hydrogel matrix, hanging drop, low attachment surface, scaffold, levitation, liquid marble, and 3D printing. These systems demonstrate satisfactory results in follicle culture, allowing the culture from the pre-antral to antral phase, maintaining the follicular morphology, and increasing the development rates of embryos. Here, we review some of the different techniques of 3D culture systems and their applications to the culture of follicles and embryos, bringing new possibilities to the future of assisted reproduction.

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Section: D Culture Applied To the Female Reproductive Systemmentioning

confidence: 99%

3D culture applied to reproduction in females: possibilities and perspectives

Ferronato,

Vit,

Silveira

2024

Anim. Reprod.

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“…Its components encompass seeding cells, biomaterial scaffolds, dynamic culture conditions, and micro-physiological environment simulations. By creating artificial organs, it can regenerate damaged or diseased tissue and restore normal organ function [ 11 , 12 ], which provides a promising approach to addressing the limitations of current reproductive therapies and improving patient outcomes. The development of tissue engineering scaffolds, the application of microfluidic chip engineering technology, decellularized extracellular matrix [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Revolutionizing the female reproductive system research using microfluidic chip platform

Yan,

Wu,

Zhang

et al. 2023

J Nanobiotechnol

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Comprehensively understanding the female reproductive system is crucial for safeguarding fertility and preventing diseases concerning women's health. With the capacity to simulate the intricate physio- and patho-conditions, and provide diagnostic platforms, microfluidic chips have fundamentally transformed the knowledge and management of female reproductive health, which will ultimately promote the development of more effective assisted reproductive technologies, treatments, and drug screening approaches. This review elucidates diverse microfluidic systems in mimicking the ovary, fallopian tube, uterus, placenta and cervix, and we delve into the culture of follicles and oocytes, gametes’ manipulation, cryopreservation, and permeability especially. We investigate the role of microfluidics in endometriosis and hysteromyoma, and explore their applications in ovarian cancer, endometrial cancer and cervical cancer. At last, the current status of assisted reproductive technology and integrated microfluidic devices are introduced briefly. Through delineating the multifarious advantages and challenges of the microfluidic technology, we chart a definitive course for future research in the woman health field. As the microfluidic technology continues to evolve and advance, it holds great promise for revolutionizing the diagnosis and treatment of female reproductive health issues, thus propelling us into a future where we can ultimately optimize the overall wellbeing and health of women everywhere. Graphical Abstract

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Bioengineered 3D ovarian model for long-term multiple development of preantral follicle: bridging the gap for poly(ε-caprolactone) (PCL)-based scaffold reproductive applications

Di Berardino,

Peserico,

Camerano Spelta Rapini

et al. 2024

Reprod Biol Endocrinol

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Background Assisted Reproductive Technologies (ARTs) have been validated in human and animal to solve reproductive problems such as infertility, aging, genetic selection/amplification and diseases. The persistent gap in ART biomedical applications lies in recapitulating the early stage of ovarian folliculogenesis, thus providing protocols to drive the large reserve of immature follicles towards the gonadotropin-dependent phase. Tissue engineering is becoming a concrete solution to potentially recapitulate ovarian structure, mostly relying on the use of autologous early follicles on natural or synthetic scaffolds. Based on these premises, the present study has been designed to validate the use of the ovarian bioinspired patterned electrospun fibrous scaffolds fabricated with poly(εcaprolactone) (PCL) for multiple preantral (PA) follicle development.Methods PA follicles isolated from lamb ovaries were cultured on PCL scaffold adopting a validated single-follicle protocol (Ctrl) or simulating a multiple-follicle condition by reproducing an artificial ovary engrafted with 5 or 10 PA (AO 5PA and AO 10PA ). The incubations were protracted for 14 and 18 days before assessing scaffold-based microenvironment suitability to assist in vitro folliculogenesis (ivF) and oogenesis at morphological and functional level. ResultsThe ivF outcomes demonstrated that PCL-scaffolds generate an appropriate biomimetic ovarian microenvironment supporting the transition of multiple PA follicles towards early antral (EA) stage by supporting follicle growth and steroidogenic activation. PCL-multiple bioengineering ivF (AO 10PA ) performed in long term generated, in addition, the greatest percentage of highly specialized gametes by enhancing meiotic competence, large chromatin remodeling and parthenogenetic developmental competence.

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IVM Advances for Early Antral Follicle-Enclosed Oocytes Coupling Reproductive Tissue Engineering to Inductive Influences of Human Chorionic Gonadotropin and Ovarian Surface Epithelium Coculture

Cited by 3 publications

References 130 publications

3D culture applied to reproduction in females: possibilities and perspectives

3D culture applied to reproduction in females: possibilities and perspectives

Revolutionizing the female reproductive system research using microfluidic chip platform

Bioengineered 3D ovarian model for long-term multiple development of preantral follicle: bridging the gap for poly(ε-caprolactone) (PCL)-based scaffold reproductive applications

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