Probiotics Prevent Intestinal Barrier Dysfunction in Acute Pancreatitis in Rats via Induction of Ileal Mucosal Glutathione Biosynthesis

Lutgendorff, Femke; Nijmeijer, Rian M.; Sandström, Per; Trulsson, Lena; Magnusson, Karl‐Eric; Timmerman, Harro M.; Minnen, L. Paul van; Rijkers, Ger T.; Gooszen, Hein G.; Akkermans, L.M.A.; Söderholm, Johan D.

doi:10.1371/journal.pone.0004512

Cited by 114 publications

(80 citation statements)

References 48 publications

(84 reference statements)

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“…LR administration in the present study also enhanced the activities of SOD, CAT, and GPx in the liver and RBCs signifying improved free radical clearance system. Previous studies have shown that oral administration of lactobacilli and fermented milk whey promotes glutathione biosynthesis in tissues which could explain increased GPx activity in LR-fed groups (Zommara et al 1998;Lutgendorff et al 2009). The liver is the major site of glutathione synthesis and transport and thus could explain the apparent effects of LR consumption on GPx activity in liver and RBCs.…”

Section: Discussionmentioning

confidence: 98%

Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice

Sharma

Kapila

Dass

et al. 2014

AGE

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Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of comprehensive analysis of probiotic effects on aging physiology. The present study aimed at determining anti-immunosenescence potential of milk fermented with probiotic Lactobacillus rhamnosus (LR) in 16 months old mice by concurrent analysis of immunosenescence markers associated with Th1/Th2 profile of splenocytes, inflamm-aging in plasma, neutrophil functions and antibody response in intestine along with analysis of antioxidant enzymes in liver and red blood cells (RBCs) after feeding trials of 1 and 2 months, respectively. An enteropathogenic Escherichia coli (ATCC 14948)-based infection model in aging mice was also designed to validate protective attributes of LR. Splenocytes registered increased IFN-γ and decreased IL-4 and IL-10 production in LR-fed animals. Neutrophil respiratory burst enzymes and phagocytosis increased significantly while no aggravation in plasma levels of MCP-1 and TNF-α was observed. Further, owing to increased Th1 response, antibodies registered a decrease in IgG1/IgG2a ratio and IgE levels in LR groups. No significant variations were observed in secretory IgA and IgA + cells in the intestine. Antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase) in LR-fed groups recorded increased activities which were more pronounced in the liver than in RBCs. LR supplementation significantly reduced E. coli translocation to organs (intestine, liver, spleen, peritoneal fluid) by enhancing E. coli-specific antibodies (IgA and IgG1) and inflammatory proteins. In conclusion, LR supplementation alleviated immunosenescence-associated Th1/Th2 imbalance, improved antioxidant capacity, and enhanced resistance of aged mice to E. coli infection thereby signifying its potential in augmenting healthy aging.

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Section: Discussionmentioning

confidence: 98%

Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice

Sharma

Kapila

Dass

et al. 2014

AGE

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“…The reduction of GSH levels can hamper the ROS clearance, causing damage to cellular components (Lushchak, 2011). Several lactic acid bacteria were able to induce the biosynthesis of GSH in rat ileal mucosa or pancreas (Lutgendorff et al, 2009). Clear positive effects of probiotics have been often observed on immune or oxidative stress status when fish are subjected to stress situations.…”

Section: Discussionmentioning

confidence: 99%

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

Ramos¹,

Batista²,

Pires

et al. 2017

Animal

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Probiotic administration can be a nutritional strategy to improve the immune response and growth performance of fish. The current study aimed to evaluate the effects of a probiotic blend (Bacillus sp., Pediococcus sp., Enterococcus sp., Lactobacillus sp.) as a dietary supplement on growth performance, feed utilization, innate immune and oxidative stress responses and intestinal morphology in juvenile Nile tilapia (Oreochromis niloticus). The probiotic was incorporated into a basal diet at three concentrations: 0 g/kg (A 0 : control), 3 g/kg (A 1 : 1.0 × 10 6 colony forming unit (CFU)/g) and 6 g/kg (A 2 : 2.3 × 10 6 CFU/g diet). After 8 weeks of probiotic feeding, weight and specific growth rate where significantly higher in fish-fed A 1 diet than in fish-fed A 0 . Alternative complement in plasma was significantly enhanced in fish-fed A 2 when compared with A 0 . The hepatic antioxidant indicators were not affected by probiotic supplementation. Villi height and goblet cell counts increased significantly in the intestine of fish-fed A 1 and A 2 diets compared with A 0 . The dietary probiotic supplementation was maintained until 20 weeks of feeding. Then the selected immune parameters, digestive enzymes and apparent digestibility of diets were studied. No effect of probiotic feeding was observed after that longer period supplementation. The dietary supplementation of mixed species probiotic may constitute a valuable nutritional approach towards a sustainable tilapia aquaculture. The improvement of the immune responses and intestinal morphology play an important role in increasing growth performance, nutrient absorption and disease resistance in fish, important outcomes in such a competitive and developing aquaculture sector.

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“…The Cd-binding ability of CCFM8610 appears to be important for the inhibition of intestinal Cd absorption, because it enables CCFM8610 to sequester Cd before intestinal absorption and increase Cd excretion as Lactobacillus strains are excreted through feces (17). However, as a considerable number of studies have shown that oral administration of probiotics can prevent intestinal barrier dysfunction by modulating immune responses, inducing tight-junction maturation and inhibiting abnormal necrosis of epithelial cells (18)(19)(20)(21), further evidence is needed to establish whether probiotics, such as CCFM8610, can inhibit Cd absorption by protecting the intestinal barrier.…”

mentioning

confidence: 99%

Oral Administration of Probiotics Inhibits Absorption of the Heavy Metal Cadmium by Protecting the Intestinal Barrier

Zhai

Tian

Zhang

et al. 2016

Appl Environ Microbiol

163

106

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The heavy metal cadmium (Cd) is an environmental pollutant that causes adverse health effects in humans and animals. Our previous work demonstrated that oral administration of probiotics can significantly inhibit Cd absorption in the intestines of mice, but further evidence is needed to gain insights into the related protection mode. The goal of this study was to evaluate whether probiotics can inhibit Cd absorption through routes other than the Cd binding, with a focus on gut barrier protection. In the in vitro assay, both the intervention and therapy treatments of

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Probiotics Prevent Intestinal Barrier Dysfunction in Acute Pancreatitis in Rats via Induction of Ileal Mucosal Glutathione Biosynthesis

Cited by 114 publications

References 48 publications

Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice

Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice

Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia

Oral Administration of Probiotics Inhibits Absorption of the Heavy Metal Cadmium by Protecting the Intestinal Barrier

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