Probiotics as an alternative antimicrobial therapy: Current reality and future directions

Silva, Diego Romário; Sardi, Janaína de Cássia Orlandi; Pitangui, Nayla de Souza; Roque, Sindy Magri; Silva, Andréa Cristina Barbosa da; Rosalen, Pedro Luiz

doi:10.1016/j.jff.2020.104080

Cited by 150 publications

(83 citation statements)

References 111 publications

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“…Probiotics are gaining popularity worldwide and there is an increasing market for functional food and feed calls for novel probiotics [10,11]. Among the known probiotics, lactic acid bacteria (LAB) (e.g., Lactococcus and Lactobacillus) and Bifidobacterium have a long utilization history, while new probiotics such as yeast and Bacillus and more specific LAB strains are being continuously identified [9,[11][12][13][14][15]. In a recent report, the probiotic value of Bacillus was underscored due to its elimination of dangerous pathogen Staphylococcus aureus via interference of S. aureus quorum sensing [14].…”

Section: Introductionmentioning

confidence: 99%

Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from Pericarpium Citri Reticulatae ‘Chachiensis’

et al. 2021

Microorganisms

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Pericarpium Citri Reticulatae ‘Chachiensis’ (PCR-Chachiensis), the pericarps of Citri Reticulatae Blanco cv. Chachiensis, is a food condiment and traditional medicine in southeast and eastern Asia. Its rich and various bacterial community awaits exploration. The present study is the first report on probiotic screening and characterization of bacteria from PCR-Chachiensis. Based on 64 culturable bacterial isolates, 8 strains were screened out to have great survival in the simulated gastrointestinal stressful condition, being nonhemolytic and without biogenic amine formation. They were identified by 16S rRNA gene sequencing as two Bacillus, three Lactobacillus, and three strains from Bacillales. Their probiotic properties, cholesterol-lowering potential and carbohydrate utilization capability were further investigated. Though these eight strains all displayed distinct cholesterol removal potential, Bacillus licheniformis N17-02 showed both remarkable cholesterol removal capability and presence of bile salt hydrolase gene, as well as possessing most of the desirable probiotic attributes. Thus, it could be a good probiotic candidate with hypocholesterolemic potential. Bacillus megaterium N17-12 displayed the widest carbohydrate utilization profile and the strongest antimicrobial activity. Hence, it was promising to be used as a probiotic in a host and as a fermentation starter in fermented food or feed.

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Section: Introductionmentioning

confidence: 99%

Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from Pericarpium Citri Reticulatae ‘Chachiensis’

et al. 2021

Microorganisms

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“…The use of probiotics not just as supplements but as actual treatment strategies for various diseases has now become more prevalent and the current focus of attention in the scientific and medical communities. The multidimensional effects of probiotics are currently being evaluated in many fields of medicine that include infectious diseases (Anwar et al, 2020;d'Ettorre et al, 2020;Silva et al, 2020;Tan et al, 2020), the immune system (Gill et al, 2000;Dargahi et al, 2020), chronic diseases such as cancer (Haghshenas et al, 2014;Nami et al, 2015), cardiovascular (Ettinger et al, 2014;Gómez-Guzmán et al, 2015;Daliri et al, 2017), neurodegenerative (Westfall et al, 2017), inflammatory diseases (Plaza-Díaz et al, 2017), and diabetes (Kocsis et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Novel Probiotic Lactobacillus and Enterococcus Strains From Human Salivary and Fecal Sources

et al. 2020

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Probiotics are non-pathogenic microorganisms that can interact with the gastrointestinal microbiota. They have numerous beneficial health effects that include enhancement of the host immune response, antiallergic, antimicrobial, anti-cancer, and anti-inflammatory properties. Probiotics are capable of restoring the impaired microbiome of a dysbiotic gut. They can be isolated from different environments. However, it is frequently suggested that probiotics for human use should come from human sources. The objective of this study was to isolate and characterize novel probiotic strains from the saliva and feces of healthy human individuals. To meet the criteria for probiotic attributes, the isolates were subjected to numerous standard morphological and biochemical tests. These tests included Gram staining, catalase tests, antibiotic susceptibility testing, hemolytic and antagonistic evaluation, tolerance tests involving temperature, NaCl levels, pH and bile salts, adherence ability assays, and genotypic characterization involving 16S rRNA gene sequencing. From 26 saliva and 11 stool samples, 185 microbial strains were isolated. Based on morphological and biochemical characteristics, 14 potential probiotic candidates were selected and identified genotypically. The new strains belonged to Lactobacillus fermentum, Enterococcus faecium, and Enterococcus hire. The selected strains were non-hemolytic, showed high tolerance to low pH and bile salts, and strong adherence abilities. Furthermore, the strains displayed a wide range of antimicrobial activities, particularly against antibiotic-resistant pathogens such as methicillin resistant Staphylococcus aureus (MRSA). Moreover, five of the selected isolates demonstrated antiproliferative features against human colon cancer cell line (Caco-2). The results of this investigation confirm the diversity of microbial populations in the human gut and saliva, and since these strains are of human origin, they will highly likely display maximal activities in food and drugs set for human use. Hence, the new strains of this study require additional in vivo experiments to assess their health-promoting effects.

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“…Microbe-based solutions have huge potential as alternatives to synthetic drugs [ 156 , 158 , 159 ]. However, they can sometimes have off-target effects.…”

Section: Promotion Of Pathogen Invadersmentioning

confidence: 99%

Host microbiota can facilitate pathogen infection

2021

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Animals live in symbiosis with numerous microbe species. While some can protect hosts from infection and benefit host health, components of the microbiota or changes to the microbial landscape have the potential to facilitate infections and worsen disease severity. Pathogens and pathobionts can exploit microbiota metabolites, or can take advantage of a depletion in host defences and changing conditions within a host, to cause opportunistic infection. The microbiota might also favour a more virulent evolutionary trajectory for invading pathogens. In this review, we consider the ways in which a host microbiota contributes to infectious disease throughout the host’s life and potentially across evolutionary time. We further discuss the implications of these negative outcomes for microbiota manipulation and engineering in disease management.

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Probiotics as an alternative antimicrobial therapy: Current reality and future directions

Cited by 150 publications

References 111 publications

Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from Pericarpium Citri Reticulatae ‘Chachiensis’

Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from Pericarpium Citri Reticulatae ‘Chachiensis’

Isolation of Novel Probiotic Lactobacillus and Enterococcus Strains From Human Salivary and Fecal Sources

Host microbiota can facilitate pathogen infection

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