Probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells

Kim, Ji Yeun; Park, Myeong Soo; Ji, Geun Eog

doi:10.3748/wjg.v18.i12.1308

Cited by 24 publications

(12 citation statements)

References 47 publications

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“…Also, for L. rhamnosus GG, it was recently shown that glycosylated heteropolymeric pili are involved in such DC‐SIGN‐mediated interactions with DCs . In another study, Bifidobacterium lactis AD011 increased the production of IL‐10 in a co‐culture with DCs and IECs, whereas L. casei IBS041 and Lactobacillus acidophilus AD031 increased TGF‐β secretion in this co‐culture system . Similar results were obtained in another co‐culture system containing peripheral blood mononuclear cells and L. lactis , that is, an increased secretion of the anti‐inflammatory cytokine IL‐10 .…”

Section: Probiotic Interactions With the Immune Systemsupporting

confidence: 72%

Probiotics for the airways: Potential to improve epithelial and immune homeostasis

Martens

Pugin

Boeck

et al. 2018

Allergy

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Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefit on the host. The therapeutic effects of probiotics have been mostly studied in the gastrointestinal tract, but recent evidence points toward the potential of these bacteria to prevent and/or treat chronic airway diseases. In this review, possible mechanisms of action of probiotics in the airways are described, with a particular focus on their capacity to modulate the epithelial barrier function and their mode of interaction with the immune system. Indeed, probiotic bacteria, mostly lactobacilli, can promote the expression and regulation of tight junctions and adherence junctions, resulting in the restoration of a defective epithelial barrier. These bacteria interact with the epithelial barrier and immune cells through pattern recognition receptors, such as Toll-like receptors, which upon activation can stimulate or suppress various immune responses. Finally, the clinical potential of probiotics to treat inflammatory diseases of the upper and lower respiratory tract, and the difference between their mode of application (eg, oral or nasal) are discussed here.

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Section: Probiotic Interactions With the Immune Systemsupporting

confidence: 72%

Probiotics for the airways: Potential to improve epithelial and immune homeostasis

Martens

Pugin

Boeck

et al. 2018

Allergy

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“…Our current findings of enhanced NO and cytokine production are in accordance with a previous report by Jung et al [47] demonstrating similar effects of Lactobacillus sakei KO40706 on RAW264.7 macrophages and the enhancement of NO and cytokine secretion (mainly TNF-α and IL-6) in mice immunosuppressed by cyclophosphamide treatment. Similarly, several strains of Lactobacillus have been shown to trigger the release of TNF-α, IL-6, and IL-10 from macrophages and dendritic cells [48]. The efficacy of probiotics to enhance the production of cytokines such as IL-12 and IL-10 is considered a critical attribute for health promotion and possible therapeutic application.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

et al. 2020

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Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-β) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain.

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“…Apart from modulating the gut microbiota, we should not overlook the potential beneficial effect exerted directly from our probiotics. In vitro experiments showed that viable EcN and VSL#3 were potent inducers of IL-10 (11), whereas LGG stimulate IL-12 production from dendritic cells (42). These probiotics have shown potent effects in prevention and treatment of gut inflammation and IL-17-mediating autoimmune diseases (11).…”

Section: Discussionmentioning

confidence: 99%

Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

Sung

Lee

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

462

386

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The beneficial roles of probiotics in lowering the gastrointestinal inflammation and preventing colorectal cancer have been frequently demonstrated, but their immunomodulatory effects and mechanism in suppressing the growth of extraintestinal tumors remain unexplored. Here, we adopted a mouse model and metagenome sequencing to investigate the efficacy of probiotic feeding in controlling s.c. hepatocellular carcinoma (HCC) and the underlying mechanism suppressing the tumor progression. Our result demonstrated that Prohep, a novel probiotic mixture, slows down the tumor growth significantly and reduces the tumor size and weight by 40% compared with the control. From a mechanistic point of view the down-regulated IL-17 cytokine and its major producer Th17 cells, whose levels decreased drastically, played critical roles in tumor reduction upon probiotics feeding. Cell staining illustrated that the reduced Th17 cells in the tumor of the probiotictreated group is mainly caused by the reduced frequency of migratory Th17 cells from the intestine and peripheral blood. In addition, shotgun-metagenome sequencing revealed the crosstalk between gut microbial metabolites and the HCC development. Probiotics shifted the gut microbial community toward certain beneficial bacteria, including Prevotella and Oscillibacter, that are known producers of antiinflammatory metabolites, which subsequently reduced the Th17 polarization and promoted the differentiation of antiinflammatory Treg/Tr1 cells in the gut. Overall, our study offers novel insights into the mechanism by which probiotic treatment modulates the microbiota and influences the regulation of the T-cell differentiation in the gut, which in turn alters the level of the proinflammatory cytokines in the extraintestinal tumor microenvironment.H epatocellular carcinoma (HCC) is one of the most common cancers, the sixth most common neoplasm, and the second most deadly type of cancer worldwide (1). The traditional HCC treatment, including surgical treatment, local ablation therapy, and chemotherapy, could offer potential cure, yet patients are facing many limitations including the poor hepatic reserve. HCC is clearly a disease for which alternative therapeutic strategies must be developed. A better understanding of the interactions between cancer cells and stromal components in the tumorassociated proinflammatory microenvironment would be important for the management of this disease.The tumor microenvironment is infiltrated with various immune cells such as T cells, macrophages, neutrophils, natural killer (NK) cells, and myeloid-derived suppressor cells. Inflammation is known to play a pivotal role in tumor development by escalating tumor angiogenesis and cell growth. Once a solid tumor is formed, inflammation arises in the tumor-promoting direction. At the same time, new vasculature is needed in the tumor to provide nutrients and oxygen to support the growth of cancer cells, and this process plays a critical role in HCC, a highly vascularized tumor (2). Inflammation and angiogene...

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Probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells

Abstract: These results suggest that specific probiotic strains exert differential immune modulation mediated by the interaction of dendritic cells and epithelial cells in the homeostasis of gastrointestinal tract.

Cited by 24 publications

References 47 publications

Probiotics for the airways: Potential to improve epithelial and immune homeostasis

Probiotics for the airways: Potential to improve epithelial and immune homeostasis

Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

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