Probiotics for the airways: Potential to improve epithelial and immune homeostasis

Martens, Katleen; Pugin, Benoı̂t; Boeck, Ilke De; Spacova, Irina; Steelant, Brecht; Seys, Sven; Lebeer, Sarah; Hellings, Peter

doi:10.1111/all.13495

Cited by 68 publications

(86 citation statements)

References 98 publications

(206 reference statements)

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“…3 Integrin β7 is required for transmigration of mouse MCp into the lung. 4 Thus, we speculate that integrin β7 is upregulated on the human MCp upon release from the BM in preparation for their transmigration from the blood to the peripheral tissues.…”

Section: Demonstration Of Human Mast Cell Progenitors In the Bone Marrowmentioning

confidence: 85%

Effect of Lactobacillus plantarum YIT 0132 on Japanese cedar pollinosis and regulatory T cells in adults

Suzuki

Kubota

Kakiyama

et al. 2019

Allergy

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Section: Demonstration Of Human Mast Cell Progenitors In the Bone Marrowmentioning

confidence: 85%

Effect of Lactobacillus plantarum YIT 0132 on Japanese cedar pollinosis and regulatory T cells in adults

Suzuki

Kubota

Kakiyama

et al. 2019

Allergy

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“…Next to this, (alternatively activated) macrophage infiltration in (polyp) tissue in chronic rhinosinusitis with nasal polyps could play a role in disease perpetuation (30)(31)(32)34). Through the enforcement of an inflammatory response or the induction of a more immunotolerant environment upon repeated exposure (35,77,78), the tissue macrophages can, directly and indirectly through the epithelium, shape the microbial community by preventing the outgrowth of particular bacteria. This relationship is bidirectional, since the resident microbiota and its metabolites will induce (anti-)inflammatory responses in the human host.…”

Section: Discussionmentioning

confidence: 99%

Dual and Triple Epithelial Coculture Model Systems with Donor-Derived Microbiota and THP-1 Macrophages To Mimic Host-Microbe Interactions in the Human Sinonasal Cavities

Rudder

Calatayud

Lebeer

et al. 2020

mSphere

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The epithelium of the human sinonasal cavities is colonized by a diverse microbial community, modulating epithelial development and immune priming and playing a role in respiratory disease. Here, we present a novel in vitro approach enabling a 3-day coculture of differentiated Calu-3 respiratory epithelial cells with a donor-derived bacterial community, a commensal species (Lactobacillus sakei), or a pathobiont (Staphylococcus aureus). We also assessed how the incorporation of macrophage-like cells could have a steering effect on both epithelial cells and the microbial community. Inoculation of donor-derived microbiota in our experimental setup did not pose cytotoxic stress on the epithelial cell layers, as demonstrated by unaltered cytokine and lactate dehydrogenase release compared to a sterile control. Epithelial integrity of the differentiated Calu-3 cells was maintained as well, with no differences in transepithelial electrical resistance observed between coculture with donor-derived microbiota and a sterile control. Transition of nasal microbiota from in vivo to in vitro conditions maintained phylogenetic richness, and yet a decrease in phylogenetic and phenotypic diversity was noted. Additional inclusion and coculture of THP-1-derived macrophages did not alter phylogenetic diversity, and yet donor-independent shifts toward higher Moraxella and Mycoplasma abundance were observed, while phenotypic diversity was also increased. Our results demonstrate that coculture of differentiated airway epithelial cells with a healthy donor-derived nasal community is a viable strategy to mimic host-microbe interactions in the human upper respiratory tract. Importantly, including an immune component allowed us to study host-microbe interactions in the upper respiratory tract more in depth. IMPORTANCE Despite the relevance of the resident microbiota in sinonasal health and disease and the need for cross talk between immune and epithelial cells in the upper respiratory tract, these parameters have not been combined in a single in vitro model system. We have developed a coculture system of differentiated respiratory epithelium and natural nasal microbiota and incorporated an immune component. As indicated by absence of cytotoxicity and stable cytokine profiles and epithelial integrity, nasal microbiota from human origin appeared to be well tolerated by host cells, while microbial community composition remained representative for that of the human (sino)nasal cavity. Importantly, the introduction of macrophage-like cells enabled us to obtain a differential readout from the epithelial cells dependent on the donor microbial background to which the cells were exposed. We conclude that both model systems offer the means to investigate host-microbe interactions in the upper respiratory tract in a more representative way.

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“…This study highlights the clinical relevance of probiotic treatment for mucosal inflammation in the sinuses. The potential therapeutic effects of probiotics on mucosal airway diseases are summarised in a recent review (72) .…”

Section: Louis Mo Usa) Labs In Collaboration With Researchers At Thementioning

confidence: 99%

Animal models for inflammatory mucosal disease and their potential for studying the microbiome in chronic rhinosinusitis

Lux¹,

Douglas²,

Cho³

et al. 2019

RHINOL

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Chronic rhinosinusitis (CRS) is a morbid condition of the paranasal sinuses which severely impairs patients' quality of life. CRS represents one of the leading diseases that are responsible for antibiotic prescriptions. However, there is little evidence to support the efficacy of antibiotics in CRS. Due to the highly heterogeneous nature of CRS determining the underlying etiology is challenging. The mucosal microbiome has been hypothesised to play a role in the pathophysiology of CRS.Several attempts to establish a representative model of CRS have been made to help determine the pathogenesis of this condition. This review summarises the current literature on model systems for inflammatory sinus disease. Fourteen different studies are discussed, including mouse, rabbit and sheep as model organisms. A detailed description of the methods for model development and examples for their application are provided. Focus is put on animal models that should be suitable for studying the sinonasal microbiome in CRS. To date, only two studies sought to employ their model for microbiome analysis. Other models are included for which there is currently no microbiome information, however they are of potential use in this regard and we thus discuss their suitability.This review identifies a need for further employment of animal models of CRS for microbiome research. Recently, a rabbit model of CRS featuring several qualities that make it particularly suitable for microbiological research has been described. This model system represents a further advance of translational research in the field of CRS.

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Probiotics for the airways: Potential to improve epithelial and immune homeostasis

Cited by 68 publications

References 98 publications

Effect of Lactobacillus plantarum YIT 0132 on Japanese cedar pollinosis and regulatory T cells in adults

Effect of Lactobacillus plantarum YIT 0132 on Japanese cedar pollinosis and regulatory T cells in adults

Dual and Triple Epithelial Coculture Model Systems with Donor-Derived Microbiota and THP-1 Macrophages To Mimic Host-Microbe Interactions in the Human Sinonasal Cavities

Animal models for inflammatory mucosal disease and their potential for studying the microbiome in chronic rhinosinusitis

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