Probiotic mixture reduces gut inflammation and microbial dysbiosis in children with atopic dermatitis

Abstract

Background: Recent data suggested that dysbiosis of the gut microbiome is associated with childhood allergic diseases. Oral administration of probiotic formulations may improve the severity of atopic dermatitis (AD) by restoring imbalanced gut microbiota and reducing intestinal inflammation in children. Objectives:The aim of this study was to investigate the effects of a probiotic mixture on the clinical severity of AD, gut inflammatory markers and alterations in microbiome dysbiosis in children with AD.Methods: A total of 25 subjects were enrolled in this study and administered with a mixture of probiotic strains consisting of Lactobacilli and Bifidobacteria for 4 weeks. The clinical efficacy of the probiotic mixture was assessed using SCORAD index and TEWL. Faecal calprotectin levels were measured as a marker for intestinal inflammation. The composition and diversity of the gut microbiome were analysed using 16S rRNA pyrosequencing. Results:The SCORAD (38.9 AE 17.2 vs 29.0 AE 15.4, P < 0.001) and TEWL (58.3 AE 12.5 vs 27.3 AE 8.7 g/ m 2 /h, P = 0.028) were significantly decreased after 4 weeks administration of the probiotic mixture. The faecal calprotectin level (121.5 [27.7-292.9] vs 37.0 lg/g [12.6-108.9 lg/g], P = 0.038) was significantly decreased. The a-diversity and composition of the gut microbiome were not significantly changed, but b-diversity was increased after 4 weeks. Conclusions:The oral administration of the probiotic mixture was effective in reducing clinical severity and intestinal inflammation in children with AD. Gut microbial diversity was slightly increased after administration of the probiotic mixture. The results of this study suggest that a probiotic mixture can alleviate AD by decreasing inflammation and modulating the gut microbiota in children with AD.

The Probiotic Lactobacillus paracasei Ameliorates Diarrhea Cause by Escherichia coli O8via Gut Microbiota Modulation1

“…A combination of L. paracasei GMNL-89 plus L. reuteri GMNL-89 was administered as an adjuvant to control cancer progression, lower serum liver enzyme levels, and improve patient chemotherapy tolerance ( 45 ). Another study showed that a mixture of probiotic Lactobacilli and Bifidobacteria alleviated atopic dermatitis by modulating the gut microbiota ( 46 ). Compared with ciprofloxacin, probiotic L. paracasei improved intestinal barrier function, inhibited pathogen invasion, and increased intestinal microbial diversity.…”

Gut microbiota and atopic dermatitis in children: a scoping review

“…The skin and the gut are the two main micro-ecosystems of the human body. Some studies have found that compared with the normal population, the diversity of skin and intestinal microbiota in children with AD is reduced before the onset of the disease, which can lead to a shift in the immune response to the T helper 2 (Th2) and increase the possibility of AD onset [ 20 , 21 ].…”

Investigation of lactic acid bacterial profiles in commercial rice wine and their effect on metabolites during low-temperature storage

“…To isolate DNA from the samples, an AccuFAST automated system (AccuGene, Incheon, Korea) was used according to the manufacturer’s instructions. The V3-V4 region of the 16S rRNA gene was amplified as previously described ( Yoon et al, 2021 ) using KAPA HiFi HosStart ReadyMix (Kapa Biosystems, Wilmington, MA, USA) and primers 341f/805r ( King et al, 2019 ) containing Nextera adaptors. The PCR products (∼428 bp) were purified using HiAccuBeads (AccuGene, South Korea).…”