Probiotic Lactobacillus strains from Mongolia improve calcium transport and uptake by intestinal cells in vitro

Raveschot, Cyril; Coutte, François; Frémont, Marc; Vaeremans, Maxime; Dugersuren, Jamyan; Demberel, Shirchin; Drider, Djamel; Dhulster, Pascal; Flahaut, Christophe; Cudennec, Benoît

doi:10.1016/j.foodres.2020.109201

Cited by 38 publications

(32 citation statements)

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“…The mitochondria absorb the Ca2 + released by the ER, and then the permeability transition pore is opened to release cytochrome c and activate the apoptotic caspase cascade [39]. At the cellular level, Lactobacillus plantarum ST-III promotes intracellular Ca 2+ assimilation [40]. Therefore, we hypothesized that L-P-cs' protective effect on the liver would also be mediated by promoting intracellular Ca 2+ assimilation to reduce ERS.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

Xu¹,

et al. 2021

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Background: Acute liver injury (ALI) involves excessive oxidative stress(OS) and inflammatory responses, leading to a high mortality rate due to lack of effective therapy. Carbon tetrachloride (CCl4) is widely used to induce ALI by induction of reactive oxygen species. Probiotics, including Lactobacillus plantarum ST-III, have been shown to produce antibacterial and antioxidant substances such as organic acids or bacteriocins that reduce liver damage. Nevertheless, the effect of Lactobacillus plantarum ST-III culture supernatant (L-P-cs) on CCl4-induced liver injury remains unclear.Methods: Mice were pretreated with L-P-cs or medium for 14days before one dose of 0.2% CCl4 at 10ml/kg body weight delivered by intraperitoneal injection. CCl4-induced liver injury was examined by measuring serum levels of liver transaminases and high mobility-group box 1 protein (HMGB1) and liver histological staining. Inflammation and apoptosis in liver were evaluated by real-time PCR, enzyme-linked immunosorbent assay (ELISA), and TUNEL staining. Apoptosis in NCTC 1469 cells was detected using CCK8 and western blotting (WB). In liver, OS and endoplasmic reticulum stress(ERS)-related proteins were measured using kits and WB.Results: L-P-cs significantly ameliorated CCl4-induced liver injury and reduced CCl4- induced inflammatory response and apoptosis, consistent with NCTC 1469 cells' results. L-P-cs also restored CCl4-induced increases in cell OS and ERS to normalize liver function. Specifically, L-P-cs pretreatment decreased CCl4-induced increases in nuclear factor (erythroid-2 related) factor 2, HO-1, superoxide dismutase, glucose regulatory protein, and activating transcription factor 6.Conclusion: L-P-cs synergistically improves liver lobule necrosis, hepatocyte inflammation, and apoptosis by reducing liver OS and ERS.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

Xu¹,

et al. 2021

Preprint

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“…Probiotics can stimulate the quantitative or qualitative composition of the intestinal microflora [ 23 ] to improve calcium [ 24 ] and magnesium [ 16 ] bioavailability. The probiotics produce short-chain fatty acids, which increase the solubility of available calcium, decrease the parathyroid hormone level, and reduce bone loss.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Multispecies Probiotics on Calcium and Magnesium Status in Healthy Male Rats

et al. 2021

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Although probiotics have been discovered in numerous diseases in the last decade, there is little consensus on the relationship between probiotic properties and minerals balance and their distribution in the organism. This research aimed to evaluate the calcium (Ca) and magnesium (Mg) status in rats on a diet containing multispecies probiotics. Thirty male 10-week-old Wistar rats were selected and divided into three groups (n = 10 rats)—a group fed a standard diet (C), a group fed a low-dose of multispecies probiotics with 2.5 × 109 CFU per day (LD), and a group fed high-dose of multispecies probiotics 1 × 1010 CFU per day (HD) for 6 weeks. The results revealed that HD intake significantly increased the Ca concentration in hair and Mg concentration in femur bones. A significant positive correlation was found between calcium and magnesium levels in hair. The Ca/Mg molar ratio was lower in testicles in the groups with probiotics. In conclusion, multispecies probiotics altered the Ca concentration in hair and Mg level in femur bone, and also changed the molar ratio of these elements in testicles in male rats.

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“…However, by means of a spectrophotometric technique, the aggregative profile of various LAB strains has been characterized. In particular, high percentages of auto-aggregation have been reported for Lactiplantibacillus strains: 28.27 ± 9.7% for L. plantarum 46a (Raveschot et al, 2020); around a 40 % for L. plantarum L15 (Alizadeh Behbahani et al, 2019), and within a range of 29.47 ± 2.63% to 33.57 ± 2.21% in other L. plantarum strains. Therefore, our results seem to be those expected for L. plantarum strains and indicate a good autoaggregation capability of M5MA1-B2 and M9MG6-B2, which could have a certain relationship with their good adhesion to the biotic Caco-2 cells surface and the high capacity to form biofilms on abiotic surfaces, characteristics that would give them certain advantages to colonize the intestine.…”

Section: Auto-aggregation Capability Of Labeled L Plantarum M5ma1-b2mentioning

confidence: 97%

The Ability of Riboflavin-Overproducing Lactiplantibacillus plantarum Strains to Survive Under Gastrointestinal Conditions

et al. 2020

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Riboflavin, vitamin B2, is essential for humans and has to be obtained from the diet. Some lactic acid bacteria (LAB) produce this vitamin, and they can be used for in-situ fortification of foods. This could be an alternative to supplementation with chemically synthesized vitamin, to palliate riboflavin deficiencies in specific groups of people. Moreover, if the producing LAB could survive in the gastrointestinal stress (GIT) they could be added as probiotics in this environment. In the present study we tested two riboflavin-overproducing Lactiplantibacillus plantarum strains (M5MA1-B2 and M9MG6-B2), spontaneous mutants of LAB isolated from chicha, a traditional Andean beverage. These two LAB, and also their isogenic strains M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12], expressing the mCherry protein from the pRCR12 plasmid, were evaluated in vitro under simulated GIT conditions. Among other, specifically developed protein fluorescence assays were used. The four LAB showed similar levels of adhesion (>6.0%) to Caco-2 cells, higher than that of the probiotic Lacticaseibacillus rhamnosus GG strain (4.51%). Thus, LAB biofilm formation was assessed in the labeled cells by intracellular mCherry fluorescence and in the unlabeled parental strains by crystal violet staining. Both methods detected the formation of consistent biofilms by the L. plantarum strains. The quantification of mCherry fluorescence was also used to analyze LAB auto-aggregation properties. High levels of auto-aggregation were detected for both M5MA1-B2[pRCR12] and M9MG6-B2[pRCR12]. Survival of LAB included in a commercial cereal-based food matrix (Incaparina) under GIT conditions was also evaluated. The four LAB were resistant in vitro to the stomach and intestinal stresses, and proliferated in this environment, indicating a protective and nutritional effect of the Incaparina on the bacteria. Also, M9MG6-B2 survival in the presence or absence of Incaparina was evaluated in vivo in a BALB/c mouse model. The administration of the M9MG6-B2 strain alone or together with Incaparina had no Hernández-Alcántara et al. Riboflavin-Overproducing Lactiplantibacillus Plantarum Gastrointestinal Survival adverse effect on the health, growth and/or well-being of the rodents. In addition, an increment in the villus length/crypt depth ratio was observed. The overall results obtained indicate that the LAB studied have probiotic characteristics of interest for the development of functional foods.

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Probiotic Lactobacillus strains from Mongolia improve calcium transport and uptake by intestinal cells in vitro

Cited by 38 publications

References 64 publications

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

The Impact of Multispecies Probiotics on Calcium and Magnesium Status in Healthy Male Rats

The Ability of Riboflavin-Overproducing Lactiplantibacillus plantarum Strains to Survive Under Gastrointestinal Conditions

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