“…Calcium contents in the liver, heart, kidney, pancreas, femur, brain, and hair were measured after digestion in 65% (w/w) spectra pure HNO3 (Merck, Kenilworth, NJ, USA) using a Microwave Digestion system (Speedwave Xpert, Berghof, Eningen, Germany), following the procedure of Suliburska et al [ 23 ]. After digestion and subsequent dilution with deionized water, calcium contents in the mineral solutions were measured using flame atomic absorption spectrometry (AAS-3, Carl Zeiss, Jena, Germany).…”