Probiotic Lactobacillus paragasseri K7 Nanofiber Encapsulation Using Nozzle-Free Electrospinning

Simonič, Marjana; Slapničar, Špela; Trček, Janja; Matijašić, Bojana Bogovič; Lorbeg, Petra Mohar; Vesel, Alenka; Zemljič, Lidija Fras; Fratnik, Zdenka Peršin

doi:10.1007/s12010-023-04416-x

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…This fact has also been supported by Ma et al [3], who observed that probiotics were successfully encapsulated in gum Arabic nanofibers, owing to their compact structure. Similar results were observed by Simonič et al [16], who observed that probiotics were successfully loaded in sodium alginate and polyethylene oxide nanofibers. Zeta potential represents the electrical potential at the interface between a particle or a surface and the surrounding liquid medium.…”

Section: Encapsulation Efficiency (Ee%)supporting

confidence: 89%

Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics

Nawaz,

Irshad,

Walayat

et al. 2023

Foods

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In the current study, apple-pectin-based novel nanofibers were fabricated by electrospinning. Polyvinyl alcohol (PVA) and apple pectin (PEC) solution were mixed to obtain an optimized ratio for the preparation of electrospun nanofibers. The obtained nanofibers were characterized for their physiochemical, mechanical and thermal properties. The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). Furthermore, an assay of the in vitro viability of free and encapsulated probiotics was carried out under simulated gastrointestinal conditions. The results of TGA revealed that the PVA/PEC nanofibers had good thermal stability. The probiotics encapsulated by electrospinning showed a high survival rate as compared to free cells under simulated gastrointestinal conditions. Furthermore, encapsulated probiotics and free cells showed a 3 log (cfu/mL) and 10 log (cfu/mL) reduction, respectively, from 30 to 120 min of simulated digestion. These findings indicate that the PVA/PEC-based nanofibers have good barrier properties and could potentially be used for the improved viability of probiotics under simulated gastrointestinal conditions and in the development of functional foods.

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Section: Encapsulation Efficiency (Ee%)supporting

confidence: 89%

Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics

Nawaz,

Irshad,

Walayat

et al. 2023

Foods

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show abstract

“…The encapsulation method not only increases the survival rate of probiotics but also permits controlled release and targeted administration to particular body locations. The utilization of protein-based biopolymers for encapsulating probiotics in nanofibers has emerged as a promising approach [5].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of PVA–WPI Based Nanofiber Mats for Improved Viability of Lactobacillus rhamnosus GG

Akram,

Afzaal,

Saeed

et al. 2023

Foods

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In the current study, whey protein-based nanofibers were fabricated to encapsulate Lactobacillus rhamnosus. Purposely, different ratios of PVA (polyvinyl alcohol) and WPI (whey protein isolate) were blended to fabricate nanofibers. Nanofiber mats were characterized in terms of particle size, diameter, tensile strength, elongation at break, and loading efficiency. Morphological and molecular characterizations were carried out using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR). Moreover, in vitro viability under simulated gastrointestinal (GI) conditions and thermal stability were also assessed. The results reveal that by increasing the PVA concentration, the conductivity increased while the viscosity decreased. SEM micrographs showed that probiotics were successfully loaded within the nanofiber. The FTIR spectra show strong bonding between the encapsulating materials with the addition of probiotics. In vitro and thermal analyses revealed that the survival of encapsulated probiotics significantly (p < 0.05) improved. In a nutshell, PVA–WPI composite nanofibers have promising potential when used to enhance the viability and stability of probiotics under adverse conditions.

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Electrospinning and Electrospraying: Emerging Techniques for Probiotic Stabilization and Application

et al. 2023

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Probiotics are beneficial for human health. However, they are vulnerable to adverse effects during processing, storage, and passage through the gastrointestinal tract, thus reducing their viability. The exploration of strategies for probiotic stabilization is essential for application and function. Electrospinning and electrospraying, two electrohydrodynamic techniques with simple, mild, and versatile characteristics, have recently attracted increased interest for encapsulating and immobilizing probiotics to improve their survivability under harsh conditions and promoting high-viability delivery in the gastrointestinal tract. This review begins with a more detailed classification of electrospinning and electrospraying, especially dry electrospraying and wet electrospraying. The feasibility of electrospinning and electrospraying in the construction of probiotic carriers, as well as the efficacy of various formulations on the stabilization and colonic delivery of probiotics, are then discussed. Meanwhile, the current application of electrospun and electrosprayed probiotic formulations is introduced. Finally, the existing limitations and future opportunities for electrohydrodynamic techniques in probiotic stabilization are proposed and analyzed. This work comprehensively explains how electrospinning and electrospraying are used to stabilize probiotics, which may aid in their development in probiotic therapy and nutrition.

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Probiotic Lactobacillus paragasseri K7 Nanofiber Encapsulation Using Nozzle-Free Electrospinning

Cited by 4 publications

References 45 publications

Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics

Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics

Fabrication and Characterization of PVA–WPI Based Nanofiber Mats for Improved Viability of Lactobacillus rhamnosus GG

Electrospinning and Electrospraying: Emerging Techniques for Probiotic Stabilization and Application

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