Probiotic Lactobacillus casei Expressing Human Lactoferrin Elevates Antibacterial Activity in the Gastrointestinal Tract

Chen, Hsiao‐Ling; Lai, Yu‐Ling; Chen, Chua-Shun; Chu, Te-Wei; Lin, Wei-Chih; Yen, Chih-Ching; Lin, Meei‐Yn; Tu, Ming-Yu; Chen, Chuan-Mu

doi:10.1007/s10534-010-9298-0

Cited by 50 publications

(38 citation statements)

References 48 publications

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“…Our recent studies also revealed that probiotic Lactobacillus casei expressing human LF exhibit antibacterial activity on E. coli ATCC 25922 both in vitro and in vivo (Chen et al 2010). In another previous study, we demonstrated increased villous length in the small intestines of mouse pups fed pLFenriched milk from transgenic females, compared with the length of villi in mice fed wild-type milk (Wu et al 2007).…”

Section: Animal Studiesmentioning

confidence: 87%

Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection

et al. 2011

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Escherichia coli (E. coli) are the most common aerobic gram-negative bacilli in a normal intestinal tract. They cause most of the intra-abdominal infections, wound infections associated with abdominal surgery, and septicemia. Most of these infections are of endogenous intestinal origin. Lactoferrin (LF) is an iron-binding glycoprotein found in milk and various external secretions. This protein has been found to have a number of biological functions, including antimicrobial, anti-cancer, antioxidant, and immunomodulatory effects. Partial degradation of LF by pepsin can give rise to peptides termed lactoferricin (LFcin) with more potent antimicrobial activity. LF and LFcin have been shown to inhibit the growth of a number of pathogenic bacteria (including E. coli and antibiotic-resistant strains), fungi, and even viruses in both in vitro and in vivo studies. We previously demonstrated that both recombinant porcine LF (pLF) produced from yeast and a synthetic 20-residue porcine LFcin peptide exhibit antimicrobial activity in vitro. In one of our recent studies, we performed pathogen challenges, including pathogenic E. coli, Staphylococcus aureus and Candida albicans, of the digestive tract of a transgenic milk-fed animal model. The results showed that LF has broad spectrum antimicrobial activity in the digestive tract and protects the mucosa of the small intestine from injury. Our following study also revealed that pLF as a feedstuff additive enhances avian immunity, including antibody formation and cell-mediated immunity. All of these results suggest that LF could be a novel natural protein in the treatment and prevention of infections with E. coli or antibiotic-resistant bacteria strains.

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Section: Animal Studiesmentioning

confidence: 87%

Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection

et al. 2011

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“…casei engineered to express human lactoferrin exhibited antimicrobial activity in the gastrointestinal tract against enteric pathogens both in vitro and in vivo. 81 This recombinant strain also protected from pathogen-induced tissue injury. Bioengineered probiotics were also able to control pathogen transmission by insects.…”

Section: Antimicrobial Action Of Bioengineered Probioticsmentioning

confidence: 92%

Bioengineered probiotics, a strategic approach to control enteric infections

Amalaradjou

Bhunia

2013

Bioengineered

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“…Lung tissue was fixed in 10% buffered formaldehyde (pH 7.0), embedded with paraffin, sectioned into 3 μ m sections, and examined using hematoxylin and eosin (H&E) staining as described previously [29, 30]. …”

Section: Methodsmentioning

confidence: 99%

Active Component of Danshen (Salvia miltiorrhizaBunge), Tanshinone I, Attenuates Lung Tumorigenesis via Inhibitions of VEGF, Cyclin A, and Cyclin B Expressions

Tung

Chen

Lee

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Tanshinone I (T1) and tanshinone II (T2) are the major diterpenes isolated from Danshen (Salvia miltiorrhiza Bunge). Three human lung adenocarcinoma cell lines, A549, CL1-0, and CL1-5, were treated with T1 and T2 for the in vitro antitumor test. Results showed that T1 was more effective than T2 in inhibiting the growth of lung cancer cells via suppressing the expression of VEGF, Cyclin A, and Cyclin B proteins in a dose-dependent manner. Moreover, a transgenic mice model of the human vascular endothelial growth factor-A165 (hVEGF-A 165) gene-induced pulmonary tumor was further treated with T1 for the in vivo lung cancer therapy test. T1 significantly attenuated hVEGF-A165 overexpression to normal levels of the transgenic mice (Tg) that were pretreated with human monocytic leukemia THP-1 cell-derived conditioned medium (CM). It also suppressed the formation of lung adenocarcinoma tumors (16.7%) compared with two placebo groups (50% for Tg/Placebo and 83.3% for Tg/CM/Placebo; P < 0.01). This antitumor effect is likely to slow the progression of cells through the S and G2/M phases of the cell cycle. Blocking of the tumor-activated cell cycle pathway may be a critical mechanism for the observed antitumorigenic effects of T1 treatment on vasculogenesis and angiogenesis.

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Probiotic Lactobacillus casei Expressing Human Lactoferrin Elevates Antibacterial Activity in the Gastrointestinal Tract

Cited by 50 publications

References 48 publications

Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection

Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection

Bioengineered probiotics, a strategic approach to control enteric infections

Active Component of Danshen (Salvia miltiorrhizaBunge), Tanshinone I, Attenuates Lung Tumorigenesis via Inhibitions of VEGF, Cyclin A, and Cyclin B Expressions

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