Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens

Mazanko, Maria S.; Makarenko, Maksim; Chistyakov, V. A.; Усатов, А. В.; Празднова, Е. В.; Bren, Anzhelica B.; Горлов, И.Ф.; Komarova, Z.B.; Weeks, Richard; Chikindas, Michael L.

doi:10.1007/s12602-019-9519-y

Cited by 14 publications

(7 citation statements)

References 24 publications

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“…It is well known that maintaining egg shell quality and internal composition is a top priority for the laying hen’s industry. In the study, our findings showed that adding probiotics to the diet could increase the AH and HU at the peak and late laying period, and increased eggshell thickness and strength at late laying periods, which was similar to the results in laying hens in Mazanko et al (2019) . Also, Wang et al (2021) reported that Clostridium butyricum can improve eggshell thickness and eggshell strength.…”

Section: Discussionsupporting

confidence: 83%

Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage

et al. 2023

Front. Microbiol.

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In order to investigate the effects of dietary probiotics supplementation on laying performance, egg quality, serum hormone levels, immunity, antioxidant, and gut microbiota of layers at different laying stages, a total of 168 Tianfu green shell laying hens (28-day-old) were randomly divided into 2 treatments: a non-supplemented control diet (NC), and diet supplemented with 10 g/kg of probiotics, respectively. Each treatment had 6 replicates with 14 hens per replicate. The feeding trial lasted for 54 weeks. The results showed that the supplementation of probiotics significantly increased the average egg weight, improved egg quality (p < 0.05) and ovarian development. Meanwhile, probiotics increased the serum hormone levels of E2 and FSH, and antioxidant indices T-AOC and T-SOD (p < 0.05) of laying hens at different laying stages (p < 0.05), decreased the expression of proinflammatory factors including IL-1, IL-6 and TNF-α (p < 0.05). Furthermore, using 16S rRNA sequencing, we observed that the addition of probiotics increased the distribution of Firmicutes, Bacteroidota and Synergistota at early laying period. Meanwhile, Bacteroidota, Actinobacteriota, Verrucomicrobiota and Deferribacterota showed an increasing trend at the peak of egg production. The relative abundance of Firmicutes, Desulfobacterota and Actinobacteriota were significantly increased at the late laying period. Moreover, PICRUSt2 and BugBase analysis revealed that at the late laying period, the probiotics supplementation not only enriched many significant gene clusters of the metabolism of terpenoids and polyketide, genetic information processing, enzyme families, translation, transcription, replication and repair, and nucleotide metabolism, but also decreased the proportion of potential pathogenic bacteria. To sum up, these data show that the addition of probiotics not only improves the performance, egg quality, ovarian development and immune function of laying hens at different laying period, but also improves the gut microbiota of layers, thus enhances production efficiency.

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Section: Discussionsupporting

confidence: 83%

Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage

et al. 2023

Front. Microbiol.

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“…Studies have shown that curcumincan be used as a feed additive to enhance the antioxidant properties of laying hens [14],and isoflavones in soybeans can be used as additives to increase progesterone secretion from chicken granulosa cells [15]. Probiotics have also been reported as feed additives to slow the rate of reproductive aging [16].Additionally,there are many studies that Chinese herbal medicine can be used as a safe feed additive, such as the effect of Astragalus membranaceus on the gut microbiota of laying hens [17]. Therefore, such natural additives have a broad prospect in animal production.…”

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confidence: 99%

Effect of Tea Polyphenol on Serum Hormone, Serum Enzyme Activity and Antioxidant-Related Gene Expression in Chinese Yellow Chicken under Heat Stress

Yang¹,

Lu²,

Zhu³

et al. 2019

Preprint

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The present study was conducted to evaluate the effects of dietary supplementation of tea polyphenol （TP） on serum hormone, serum enzyme activity, antioxidant-related and immune-related gene expression of laying hens under heat stress. A total of 288 Chinese yellow chicken (186 days old) were randomly distributed among two treatments, each of which included 6 replicates of 24 hens. Dietary treatments were that the basal diet was supplemented with 200 mg / kg tea polyphenol. The study lasted for 7 weeks, including 1 week of adaptation and 6 weeks of the formal test. The content of high-density lipoprotein cholesterol (HDL-C) and total protein (TP) in serum significantly decreased by dietary supplementation with tea polyphenol. Dietary tea polyphenol supplementation improved serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) enzyme activity and decreased serum malondialdehyde (MDA) content in treatments compared to the control. However, supplementation of tea polyphenol did not affect the activity of serum catalase (CAT). The results indicated that long-term feeding of tea polyphenols help to increase the amount of hormones (FSH, E2) associated with reproduction in laying hens and thus improve egg production. It also improved the immune function of laying hens in high temperature environments. Adding tea polyphenols to the diet can significantly increase the serum IgG, IgM content of the laying hens and can upregulate the IgA content. Dietary supplementation of tea polyphenols in the laying hens significantly increased the expression of antioxidant enzyme-related genes (SOD, CAT and GPX1) in the liver. Moreover, the addition of tea polyphenols significantly increased the expression of immune-related genes (Interferon-γ (INF-γ), Interleukin 2 (IL-2) and Interleukin 4 (IL-4)) in the spleen. It is concluded that addition of tea polyphenols has a positive effect on antioxidant activity and immune function of laying hens.

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“…The control of pathogens is one of the major interests for the poultry industry from an economic and public health point of view. The Bacillus DFM supplementation of layer hen diets has shown improved laying production by enhancing gut health, increasing intestinal fermentation and mineral assimilation, and improving the overall daily feed intake (14,61,62). The exact mechanism by which Bacillus DFM supplementation inhibits pathogens is not wellunderstood; the direct inhibition by the production of antimicrobial metabolites or competitive exclusion have been suggested as the main modes of action, other mechanisms such as the enhancement of the intestinal mucosal layer to avoid microbial diffusion across the membrane has also been suggested (14).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens

Ayala¹,

Grum²,

Evans³

et al. 2023

Front. Vet. Sci.

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Focal Ulcerative Dermatitis (FUDS) is an emerging dermatological disease that affects cage-free laying flocks, it is characterized by the development of a lesion on the dorsum of the birds; FUDS is sporadic in nature and can result in a drop in egg production and up to 50% of cumulative mortality. A total of two cage-free flocks (flock 1: no history of FUDS; flock 2: birds affected with FUDS) from a commercial laying hen operation in the mid-west U.S. were sampled in this study. The microbial composition of skin, cloacal, cecal, and ileal samples from each bird was characterized through next generation sequencing (NGS). Results identified Staphylococcus aureus and Staphylococcus agnetis as the potential causative agents of FUDS, being the most predominant in FUDS positive birds. These results were confirmed by plating, with both staphylococci as the only pathogens isolated from lesions of FUDS positive birds. A total of 68 confirmed Staphylococcus isolates from skin and environmental samples were further analyzed by whole genome sequencing (WGS) for the presence of antimicrobial resistance (AMR) genes and virulence factors that could have contributed to the development of FUDS. Forty-four-point one-two percent of the isolates had between one and four acquired AMR genes encoding for macrolides, lincosamides, spectrogramines, and beta-lactams resistance. Six classes of virulence factors associated with adherence, enzyme, immune evasion, secretion system, toxin, and iron uptake were identified. The antimicrobial effect of 4 proprietary Bacillus Direct Fed Microbial (DFM) combinations was evaluated against the Staphylococcus aureus and Staphylococcus agnetis isolates, by agar well-diffusion (AWD) assay and competitive exclusion (CE) on broth culture. Through this antimicrobial screening, a particular two-strain combination of Bacillus pumilus was identified as the most effective inhibitor of both staphylococci. A customized Bacillus pumilus product is being used at different farms with history of FUDS resulting in the successful inhibition of both Staphylococcus aureus and Staphylococcus agnetis, decreasing FUDS mortalities, and improving harvestable eggs.

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Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens

Cited by 14 publications

References 24 publications

Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage

Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage

Effect of Tea Polyphenol on Serum Hormone, Serum Enzyme Activity and Antioxidant-Related Gene Expression in Chinese Yellow Chicken under Heat Stress

Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens

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