Probiotic
            <i>Lactobacillus</i>
            Species and Their Biosurfactants Eliminate Acinetobacter baumannii Biofilm in Various Manners

Al-Shamiri, Mona Mohamed; Wang, Jingdan; Zhang, Sirui; Li, Pu; Odhiambo, Woodvine Otieno; Chen, Yanjiong; Han, Benyong; Yang, Eun Jin; Meng, Xianhong; Han, Lihua; Han, Shaoshan

doi:10.1128/spectrum.04614-22

Cited by 6 publications

(3 citation statements)

References 61 publications

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“…The antagonistic or antimicrobial effect of LAB against pathogens has been well documented in the literature ( 5 , 9 ). However, studies on the antimicrobial activity of LAB and/or its extracellular products against A. baumannii are currently limited ( 7 , 9 , 27 ). In the present work we observed a strong inhibitory activity of the LAB Lcb.…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic and transcriptional analysis of the antimicrobial effect of lactic acid bacteria on carbapenem-resistant Acinetobacter baumannii: Lacticaseibacillus rhamnosus CRL 2244 an alternative strategy to overcome resistance?”

Rodriguez,

Ramlaoui,

Georgeos

et al. 2023

Preprint

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Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii. The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections.

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Section: Discussionmentioning

confidence: 99%

“…rhamnosus can lead to the disruption or disintegration of the A. baumannii cell envelope. Additionally, these authors observed the emergence of filament-like structures, which can serve as sites for the excessive leakage of vital cytoplasmic contents ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and transcriptional analysis of the antimicrobial effect of lactic acid bacteria on carbapenem-resistant Acinetobacter baumannii: Lacticaseibacillus rhamnosus CRL 2244 an alternative strategy to overcome resistance?”

Rodriguez,

Ramlaoui,

Georgeos

et al. 2023

Preprint

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“…The authors showed that the observed effect was mainly exerted by lactic acid and acetic acid, which showed a strong anti-A. baumanii bactericidal effect [106]. In another study, the authors demonstrated that the Lactobacillus plantarum strain was able to interfere with P. aeruginosa infections by suppressing QS, adhesion, and biofilm formation.…”

Section: Biofilm Wound Treatmentmentioning

confidence: 98%

Bacterial Biofilm in Chronic Wounds and Possible Therapeutic Approaches

Cavallo,

Sivori,

Mastrofrancesco

et al. 2024

Biology

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Wound repair and skin regeneration is a very complex orchestrated process that is generally composed of four phases: hemostasis, inflammation, proliferation, and remodeling. Each phase involves the activation of different cells and the production of various cytokines, chemokines, and other inflammatory mediators affecting the immune response. The microbial skin composition plays an important role in wound healing. Indeed, skin commensals are essential in the maintenance of the epidermal barrier function, regulation of the host immune response, and protection from invading pathogenic microorganisms. Chronic wounds are common and are considered a major public health problem due to their difficult-to-treat features and their frequent association with challenging chronic infections. These infections can be very tough to manage due to the ability of some bacteria to produce multicellular structures encapsulated into a matrix called biofilms. The bacterial species contained in the biofilm are often different, as is their capability to influence the healing of chronic wounds. Biofilms are, in fact, often tolerant and resistant to antibiotics and antiseptics, leading to the failure of treatment. For these reasons, biofilms impede appropriate treatment and, consequently, prolong the wound healing period. Hence, there is an urgent necessity to deepen the knowledge of the pathophysiology of delayed wound healing and to develop more effective therapeutic approaches able to restore tissue damage. This work covers the wound-healing process and the pathogenesis of chronic wounds infected by biofilm-forming pathogens. An overview of the strategies to counteract biofilm formation or to destroy existing biofilms is also provided.

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Motility of Acinetobacter baumannii: regulatory systems and controlling strategies

Jeong,

Khan,

Tabassum

et al. 2023

Appl Microbiol Biotechnol

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Probiotic Lactobacillus Species and Their Biosurfactants Eliminate Acinetobacter baumannii Biofilm in Various Manners

Abstract: A. baumannii biofilm is a significant virulence factor that causes the biofilm colonization of invasive illnesses. Rising bacterial resistance to synthetic antimicrobials has prompted researchers to look at natural alternatives, such as probiotics and their derivatives.

Cited by 6 publications

References 61 publications

Phenotypic and transcriptional analysis of the antimicrobial effect of lactic acid bacteria on carbapenem-resistant Acinetobacter baumannii: Lacticaseibacillus rhamnosus CRL 2244 an alternative strategy to overcome resistance?”

Phenotypic and transcriptional analysis of the antimicrobial effect of lactic acid bacteria on carbapenem-resistant Acinetobacter baumannii: Lacticaseibacillus rhamnosus CRL 2244 an alternative strategy to overcome resistance?”

Bacterial Biofilm in Chronic Wounds and Possible Therapeutic Approaches

Motility of Acinetobacter baumannii: regulatory systems and controlling strategies

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