Probiotic <i>Lactobacilli</i> stimulate avian beta-defensin 9 expression in cultured chicken small intestinal epithelial cells

Li, G. H.; Hong, Zhimin; Jia, Y. J; You, Jinming; Zhang, J. H.; Liu, B. S.

doi:10.1017/s0029665112003308

Cited by 6 publications

(11 citation statements)

References 3 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it has been well documented that heat-killed probiotic bacteria and probioticderived products, including chromosomal DNA, cell wall components, and soluble metabolites, have the capacity to regulate local mucosal and systemic immune responses (Corthésy, Gaskins, & Mercenier, 2007;Giahi, Elmadfa, Hoseini, & Klein, 2013;Jensen, Cash, Farmer, & Keller, 2017;Lee et al, 2016;O'Flaherty, Saulnier, Pot, & Versalovic, 2010;Sanchez, Urdaci, & Margolles, 2010). We previously reported that both viable and heat-inactivated L. rhamnosus MLGA significantly promoted AvBD9 expression in chicken intestinal epithelial cells (Li et al, 2012). Based on this observation, in this study, we further investigated the specific bacterial components of the probiotic bacteria, L. rhamnosus MLGA, that was responsible for the induction of AvBD9 expression and delineated the mechanism by which L. rhamnosus MLGA and its components regulated the expression of the antimicrobial peptide AvBD9.…”

Section: Discussionmentioning

confidence: 96%

“…Our previous study demonstrated that specific probiotic Lactobacillus strains dose-dependently enhanced AvBD9 gene expression in primary intestinal epithelial cells of chicken embryos. Moreover, Lactobacillus rhamnosus MLGA from the healthy chicken small intestine induced the strongest expression of AvBD9, among all of the tested Lactobacillus strains (Li et al, 2012). Additionally, heat-inactivated L. ramous MLGA also upregulated the expression of AvBD9, indicating that specific components from this probiotic bacterium confer beneficial effects to host defense.…”

Section: Introductionmentioning

confidence: 88%

“…We previously demonstrated that both live and heat-killed L. rhamnosus MLGA induced AvBD9 mRNA expression in primary chicken intestinal epithelial cells (Li et al, 2012), indicating that specific cellular components from this probiotic bacterium induced AvBD9 expression. To determine the specific cellular components responsible for the induction of AvBD9 expression, we treated chicken intestinal epithelial cells with various L. rhamnosus MLGA preparations, including S, CWP, LTA, WCW, and WPG.…”

Section: Induction Of Avbd9 In Intestinal Epithelial Cells By Cellulamentioning

confidence: 94%

“…The probiotic Lactobacillus rhamnosus MLGA strain was isolated from the small intestine of a healthy chicken (Li et al, 2012). L. rhamnosus MLGA was cultured statically in Mann-Rogosa-Sharpe (MRS) broth (Solarbio, Beijing, China) overnight at 37°C under anaerobic conditions.…”

Section: Probiotic Bacterial Strainmentioning

confidence: 99%

See 3 more Smart Citations

Toll-like receptor 2-mediated induction of avian β -defensin 9 by Lactobacillus rhamnosus and its cellular components in chicken intestinal epithelial cells

Jia

Wei

Hong

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

This study aimed to investigate the effects of probiotic Lactobacillus rhamnosus MLGA and its cell wall components on the induction of avian β-defensin 9 (AvBD9) expression in intestinal epithelial cells (IECs) from chicken embryos and to elucidate the underlying mechanism. Chicken IECs were stimulated with L. rhamnosus MLGA or cell wall components. Quantitative RT-PCR analysis was used to determine the expressions of target genes. L. rhamnosus MLGA and its cellular components significantly induced AvBD9 and TLR2 expression, with the whole peptidoglycan (WPG) being the most potent inducer. Pretreatment of chicken IECs with a TLR2 neutralizing antibody diminished AvBD9 induction by L. rhamnosus MLGA and WPG. The inhibition tests showed that induction of AvBD9 in IECs by L. rhamnosus MLGA and WPG was dependent on NF-κB or JNK pathways. These results demonstrate that L. rhamnosus MLGA and WPG induce AvBD9 expression in chicken IECs via a TLR2-mediated NF-κB/JNK pathway.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 88%

Section: Induction Of Avbd9 In Intestinal Epithelial Cells By Cellulamentioning

confidence: 94%

Section: Probiotic Bacterial Strainmentioning

confidence: 99%

See 2 more Smart Citations

Toll-like receptor 2-mediated induction of avian β -defensin 9 by Lactobacillus rhamnosus and its cellular components in chicken intestinal epithelial cells

Jia

Wei

Hong

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…Wspomniane substancje produkowane są przez makrofagi, komórki heterofilne czy komórki nabłonka. Zaburzają przepuszczalność błony cytoplazmatycznej komórki bakteryjnej i doprowadzają do jej lizy (42,48). Najwięcej danych na temat wpływu bakterii na wytwarzanie wspomnianych substancji pochodzi z prac nad patogenami jelitowymi, jak np.…”

Section: Znaczenie Mikrobiomu Jelitowegounclassified

Chicken intestinal microbiome: Development and function

Binek¹,

Cisek²,

Rzewuska³

et al. 2017

Medycyna Weterynaryjna

View full text Add to dashboard Cite

SummaryChicken ceca contain an immense number of microorganisms collectively known as the microbiome. This community is now recognized as an essential component of the intestinal ecosystem and referred to as a metabolic organ exquisitely tuned to the host's physiology. These functions include the ability to process otherwise indigestible components of the feed, converting them into energy and body mass. The gut microbiome can also affect intestinal morphology and modulate the development and function of the immune system. This microbiota contains a rich collection of genes encoding enzymes necessary for decomposition of dietary polysaccharides and oligosaccharides, nitrogen metabolism, fatty acid and lipid metabolism, and pathways involved in a hydrogen sink. Chickens, like most animals, lack the genes for glycoside hydrolase, polysaccharide lyase, and carbohydrate esterase enzymes that are necessary to facilitate the degradation of non-starch polysaccharides. During the decomposition of dietary polysaccharides, bacteria produce short-chain (volatile) fatty acids (SCFAs), such as acetic, propionic and butyric acid. These SCFASs are absorbed transepithelially and serve as a source of energy for the host. The accumulation of molecular hydrogen released during fermentation leads to fermentation slowdown or to the production of less energy-efficient substances, such as ethanol, butyrate and propionate. The presence of bacteria that act as a hydrogen sink results in a switch to the more productive fermentation into acetate and increased production of SCFAs. Such activity could lead to a significant improvement in poultry production and the associated economics.

show abstract

Necrotic enteritis in chickens: a review of pathogenesis, immune responses and prevention, focusing on probiotics and vaccination

Alizadeh

Shojadoost

Boodhoo

et al. 2021

Anim. Health. Res. Rev.

View full text Add to dashboard Cite

Necrotic enteritis (NE), caused by Clostridium perfringens (CP), is one of the most common of poultry diseases, causing huge economic losses to the poultry industry. This review provides an overview of the pathogenesis of NE in chickens and of the interaction of CP with the host immune system. The roles of management, nutrition, probiotics, and vaccination in reducing the incidence and severity of NE in poultry flocks are also discussed.

show abstract

Probiotic Lactobacilli stimulate avian beta-defensin 9 expression in cultured chicken small intestinal epithelial cells

Cited by 6 publications

References 3 publications

Toll-like receptor 2-mediated induction of avian β -defensin 9 by Lactobacillus rhamnosus and its cellular components in chicken intestinal epithelial cells

Toll-like receptor 2-mediated induction of avian β -defensin 9 by Lactobacillus rhamnosus and its cellular components in chicken intestinal epithelial cells

Chicken intestinal microbiome: Development and function

Necrotic enteritis in chickens: a review of pathogenesis, immune responses and prevention, focusing on probiotics and vaccination

Contact Info

Product

Resources

About