Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases

Resistance to antibiotics is escalating and threatening humans and animals worldwide. Different countries have legislated or promoted the ban of antibiotics as growth promoters in livestock and aquaculture to reduce this phenomenon. Therefore, to improve animal growth and reproduction performance and to control multiple bacterial infections, there is a potential to use probiotics as non-antibiotic growth promoters. Lactic acid bacteria (LAB) offer various advantages as potential probiotics and can be considered as alternatives to antibiotics during food-animal production. LAB are safe microorganisms with abilities to produce different inhibitory compounds such as bacteriocins, organic acids as lactic acid, hydrogen peroxide, diacetyl, and carbon dioxide. LAB can inhibit harmful microorganisms with their arsenal, or through competitive exclusion mechanism based on competition for binding sites and nutrients. LAB endowed with specific enzymatic functions (amylase, protease…) can improve nutrients acquisition as well as animal immune system stimulation. This review aimed at underlining the benefits and inputs from LAB as potential alternatives to antibiotics in poultry, pigs, ruminants, and aquaculture production.

show abstract

“…A modified Lb. plantarum conferred resistance to yellow head virus in shrimps (Thammasorn et al, 2017). In piglets, Lb.…”

Section: Benefits For Animals and Food Chainmentioning

confidence: 99%

Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives to Antibiotic Growth Promoters During Food-Animal Production

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The development of adherent cells in biofilms can also encourage the fast elimination of nonadhesive cells from the microbiota (Knipe et al 2020). For instance, fed penaeid shrimp with L. plantarum strains (at 10 7 CFUs ml −1 seawater)-supplemented diet protected shrimp against V. parahaemolyticus infection (acute hepatopancreatic necrosis disease, AHPND) (Thammasorn et al 2017).…”

Section: The Protective Role Of Lactic Acid Bacteria Against Viral Diseasesmentioning

confidence: 99%

Using lactic acid bacteria as an immunostimulants in cultured shrimp with special reference to Lactobacillus spp.

et al. 2020

View full text Add to dashboard Cite

Shrimp aquaculture is the rapidly growing sector of fish culture producing sectors in the world due to its rising demand in developed countries. Moreover, the intensive and extensive shrimp culture systems are affected mainly by infection diseases caused by several pathogenic agents and resulting in high economic losses. Nowadays, controlling and preventive infection diseases are the major concern to develop this sector. Besides, the highly extensive usage of chemotherapeutics and antibiotics induced numerous drawbacks in shrimp farming, developed antibiotic resistance bacterial strains, and threatens the aquatic life. Recently, improving the immunity status and controlling infectious diseases using biological methods have become beneficial, environment friendly, and risk-free options in aquaculture. Probiotics have gained much attention as immunostimulants in aquaculture owing to their inhibitory properties on pathogenic microorganisms via creation of an unfriendly atmosphere to cease their growth. Lactobacillus species as probiotics was used in shrimp aquaculture to prevent viral infections due to their positive promoting effects on survival and health. Additionally, Lactobacillus species possesses sturdy antimicrobial activity against various pathogenic microorganisms such as Photobacterium and Vibrio infections. This review threw the light on the applications of Lactobacillus sp. as probiotics in cultured shrimp which could be of great impact from a sustainable, intensive, and ecofriendly aquaculture opinion.

show abstract

“…RNAi through dsRNA can also be applied to sanitary pests such as fire ants (Choi et al 2012), termites (Zhou et al 2008), and red flour beetle (Knorr et al 2018). Furthermore, the application of dsRNA to control viral diseases affecting the shrimp farming industry has recently been studied (Charoonnart et al 2019;Flegel 2019;Thammasorn et al 2017). We expect that cost-competitive dsRNAs produced using the production system developed in this study will contribute to various agricultural and aquacultural industries in the near future.…”

Section: Discussionmentioning

confidence: 92%

Efficient production of long double-stranded RNAs applicable to agricultural pest control by Corynebacterium glutamicum equipped with coliphage T7-expression system

Hashiro

Chikami

Kawaguchi

et al. 2021

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

RNA-based pesticides exert their function by suppressing the expression of an essential gene in the target pest through RNA interference caused by double-stranded RNA (dsRNA). Here, we selected target genes for growth suppression of the solanaceous crop pests ladybird beetle (Henosepilachna vigintioctopunctata) and Colorado potato beetle (Leptinotarsa decemlineata)-the death-associated inhibitor of apoptosis protein 1 gene (diap1), and an orthologous gene of the COPI coatomer protein complex (copI), respectively. We constructed a cost-competitive overproduction system for dsRNA using Corynebacterium glutamicum as a host bacterium. The dsRNA expression unit was equipped with two sets of promoters and terminators derived from coliphage T7, and the convergent expression system was designed to be selectively transcribed by T7 RNA polymerase. This expression system efficiently overproduced both target dsRNAs. On culture in a jar fermentor, the yield of diap1-targeting dsRNA (approximately 360 bp) was > 1 g per liter of culture. Long-chain diap1-targeting dsRNAs (up to around 1 kbp) could be produced without a substantial loss of efficiency. dsRNA accumulated in C. glutamicum significantly suppressed larval growth of H. vigintioctopunctata. The dsRNA expression technology developed here is expected to substantially reduce dsRNA production costs. Our method can be applied for a wide range of industrial uses, including agricultural pest control. Key points • Overexpression of dsRNA was achieved in C. glutamicum using a coliphage T7 system. • The best strain produced > 1 g/L of the target dsRNA species, for use as an insecticide. • The developed system efficiently produced long dsRNA species, up to ~ 1 kbp.

show abstract

Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases

Cited by 25 publications

References 38 publications

Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives to Antibiotic Growth Promoters During Food-Animal Production

Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives to Antibiotic Growth Promoters During Food-Animal Production

Using lactic acid bacteria as an immunostimulants in cultured shrimp with special reference to Lactobacillus spp.

Efficient production of long double-stranded RNAs applicable to agricultural pest control by Corynebacterium glutamicum equipped with coliphage T7-expression system

Contact Info

Product

Resources

About