Probiogenomic analysis and safety assessment of Bacillus isolates using Omics approach in combination with In-vitro

Development of microencapsulated dried Bacillus sp. 63‐11 with enhanced shelf stability and bioactivity for use as a food supplement

“…Its probiotic and pigmentation attributes were reported in Khullar et al . (2022) and the carotenoid content of fresh cells on per wet and dry basis was reported in Sricharoen et al . (2022).…”

“…Draft genome sequencing by Khullar et al . (2022) also identified crtNc (4,4′‐diapolycopene‐4,4′‐dial dehydrogenase) and crtl (phytofluene by phytoene desaturase) gene responsible for carotenoid production.…”

“…labelled code 63-11, activated from glycerol stock in nutrient broth at 37 °C, 150 x g for 48 h, was sourced from Chulalongkorn University. Its probiotic and pigmentation attributes were reported in Khullar et al (2022) and the carotenoid content of fresh cells on per wet and dry basis was reported in Sricharoen et al (2022).…”

“…Dried powder (1 g) was homogenised in phosphate‐buffered saline (9 mL) for 20 min, prior to adding simulated gastric fluid (NaCl 9 g L −1 , pepsin 3 g L −1 , pH 1.8) at 37 °C, 150 rpm for 2 h; followed by adding 10 mL simulated intestinal fluid (NaCl 9 g L −1 , pancreatin 10 g L −1 , trypsin 10 g L −1 , bile salts 3 g L −1 , pH 6.5 at 37 °C, 150 rpm for 3 h). This analysis was compared to fresh (pre‐dried) cells (Khullar et al ., 2022) and perform in triplicates on the first day of storage.…”

“…Previous research by (Khullar et al ., 2022) unveiled and characterised Bacillus infantis strain 63‐11 (Bio‐sample: SAMN22241476; Accession: NZ_JAJBAQ000000000.1), from Thai fermented fish ‘Plara’ for its resilience to heat, acidity, and bile survival and other interesting properties which emerged as a potential probiotic candidate. Additionally, the strain demonstrated intriguing orange‐red pigment production.…”