Probing vaccine antigens against bovine mastitis caused by Streptococcus uberis

Collado, Rosa; Prenafeta, Antoni; González-González, Luis; Pérez-Pons, Josep A.; Sitjà, Marta

doi:10.1016/j.vaccine.2016.05.044

Cited by 24 publications

(14 citation statements)

References 29 publications

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“…Two mastitis vaccine candidates against S. uberis have shown a significant reduction in the mortality of mice infected with the pathogen (83). Different candidates for a S. aureus vaccine is currently being investigated; in one study, live-attenuated small-colony variants have shown promising results compared to inactivated bacteria in murine models (84).…”

Section: Potential Vaccines Against Biofilm Forming Mastitis Pathogensmentioning

confidence: 99%

“…Most of the so-called in vivo investigations of biofilm in bovine mastitis have used experimental models (mice and sheep), and the majority of these studies focused on anti-biofilm treatment or vaccines against biofilm udder infections (Table 1) (44,69,70,72,(83)(84)(85). Only a few studies investigated and confirmed biofilm in vivo within udder tissue of dairy cows with bovine mastitis.…”

Section: In Vivo Investigations Of Biofilm In Bovine Mastitismentioning

confidence: 99%

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Biofilm Research in Bovine Mastitis

et al. 2021

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Bovine mastitis is one of the most important diseases in the dairy industry and has detrimental impact on the economy and welfare of the animals. Further, treatment failure results in increased antibiotic use in the dairy industry, as some of these mastitis cases for unknown reasons are not resolved despite standard antibiotic treatment. Chronic biofilm infections are notoriously known to be difficult to eradicate with antibiotics and biofilm formation could be a possible explanation for mastitis cases that are not resolved by standard treatment. This paper reviews the current literature on biofilm in bovine mastitis research to evaluate the status and methods used in the literature. Focus of the current research has been on isolates from milk samples and investigation of their biofilm forming properties in vitro. However, in vitro observations of biofilm formation are not easily comparable with the in vivo situation inside the udder. Only two papers investigate the location and distribution of bacterial biofilms inside udders of dairy cows with mastitis. Based on the current knowledge, the role of biofilm in bovine mastitis is still unclear and more in vivo investigations are needed to uncover the actual role of biofilm formation in the pathogenesis of bovine mastitis.

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Section: Potential Vaccines Against Biofilm Forming Mastitis Pathogensmentioning

confidence: 99%

Section: In Vivo Investigations Of Biofilm In Bovine Mastitismentioning

confidence: 99%

Biofilm Research in Bovine Mastitis

et al. 2021

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“…Proteomics is an important tool to identify potential vaccine antigens, and is especially useful for pathogens such as S. aureus that express numerous surface exposed proteins (Collado et al, 2016; Dwivedi et al, 2016). Studies have reported the use of 2-dimensional electrophoresis ( 2DE ) for whole or subcellular proteome analysis of Staphylococcus for human vaccine development (Sellman et al, 2005; Brady et al, 2006; Solis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Immunoproteomics to identify Staphylococcus aureus antigens expressed in bovine milk during mastitis

Misra

Holt

et al. 2018

Journal of Dairy Science

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Staphylococcus aureus is an opportunistic pathogen affecting both human and animal species. An effective vaccine to prevent S. aureus bovine disease and transmission would have positive effects on animal well-being, food production, and human health. The objective of this study was to identify multiple antigens that are immunoreactive during udder colonization and disease for exploration as vaccine antigens to prevent bovine mastitis. Staphylococcus aureus produces several cell wall-anchored and surface-associated virulence factors that play key roles in the pathogenesis of mastitis. Many of these proteins are conserved between different strains of S. aureus and represent promising vaccine candidates. We used an immunoproteomics approach to identify antigenic proteins from the surface of S. aureus. The expression of cell wall and surface proteins from S. aureus was induced under low iron conditions, followed by trypsin extraction and separation by 2-dimensional electrophoresis. The separated proteins were blotted with antibodies from mastitic bovine milk and identified by liquid chromatography-mass spectrometry. Thirty-eight unique proteins were identified, of which 8 were predicted to be surface exposed and involved in S. aureus virulence. Two surface proteins, iron-regulated surface determinant protein C (IsdC) and ESAT-6 secretion system extracellular protein (EsxA), were cloned, expressed, and purified from Escherichia coli for confirmation of immune reactivity by ELISA. A PCR of 37 bovine S. aureus isolates indicated that the presence of esxA and isdC is conserved, and amino acid alignments revealed that IsdC and EsxA sequences are highly conserved. The immunoproteomics technique used in this study generated reproducible results and identified surface exposed and reactive antigens for further characterization.

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“…S. uberis is another pathogen causing mastitis worldwide. Vaccine antigens from the protein of S. uberis were evaluated as a potential target and tested in murine models (Collado et al ., 2016).…”

Section: Control Measures and Future Aspectsmentioning

confidence: 99%

Causes, types, etiological agents, prevalence, diagnosis, treatment, prevention, effects on human health and future aspects of bovine mastitis

Ashraf

Imran

2020

Anim. Health. Res. Rev.

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Mastitis is among the most common and challenging diseases of dairy animals. It is an inflammation of udder tissues due to physical damage, chemical irritation, or infection caused by certain pathogens. Bovine mastitis has been known for ages, but its complex etiology and multi-factorial nature make it difficult to control. Mastitis may have a negative impact on human health by inducing antibiotic-resistant pathogens that may spread, which is threatening. Researchers are continuously struggling to devise suitable methods for mastitis control. Management strategies are mainly focused on disease prevention by farm management which includes proper hygiene, trained staff to monitor minor changes in the udder or milk, and better diagnostic and treatment methods. New technologies which have the potential to unravel this complicated disease include improved diagnostic tools, based on advanced genomics or proteomics, prevention, based on vaccines and immune modulators, and metabolic products of probiotics such as bacteriocins and gene therapy.

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Probing vaccine antigens against bovine mastitis caused by Streptococcus uberis

Cited by 24 publications

References 29 publications

Biofilm Research in Bovine Mastitis

Biofilm Research in Bovine Mastitis

Immunoproteomics to identify Staphylococcus aureus antigens expressed in bovine milk during mastitis

Causes, types, etiological agents, prevalence, diagnosis, treatment, prevention, effects on human health and future aspects of bovine mastitis

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