Probing the physiology of ASH neuron in <i>Caenorhabditis elegans</i> using electric current stimulation

Chokshi, Trushal Vijaykumar; Bazopoulou, Daphne; Chronis, Nikos

doi:10.1063/1.3615821

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…28 Various microfluidic devices have been reported that trap multiple animals for high-throughput imaging, 29 automated sorting, 30 quantification of muscular forces, 31 chemosensory analyses, 25,32 electrotaxis, 33,34 thermosensory analyses, 35,36 and whole-animal stress responses. 24,37 A long term culture platform for C. elegans based on microfluidic approaches has also been presented.…”

Section: Introductionmentioning

confidence: 99%

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

et al. 2017

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New tools for applying force to animals, tissues, and cells are critically needed in order to advance the field of mechanobiology, as few existing tools enable simultaneous imaging of tissue and cell deformation as well as cellular activity in live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical imaging of cellular deformations and activity while applying precise mechanical stimuli to the surface of the worm’s cuticle with a pneumatic pressure reservoir. To evaluate device performance, we compared analytical and numerical simulations conducted during the design process to empirical measurements made with fabricated devices. Leveraging the well-characterized touch receptor neurons (TRNs) with an optogenetic calcium indicator as a model mechanoreceptor neuron, we established that individual neurons can be stimulated and that the device can effectively deliver steps as well as more complex stimulus patterns. This microfluidic device is therefore a valuable platform for investigating the mechanobiology of living animals and their mechanosensitive neurons.

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Section: Introductionmentioning

confidence: 99%

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

et al. 2017

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“…Using a microfluidic chip21, we delivered a hyperosmotic stimulus (glycerol) to the worm’s nose and recorded Ca 2+ transients from the ASH sensory neuron. We chose ASH, the well-studied worms’ main nociceptor2122232425262728, because it can be activated by a wide range of repellents252930, thus being especially significant for C. elegans survival and environmental perception.…”

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confidence: 99%

Chemically induced oxidative stress affects ASH neuronal function and behavior in C. elegans

Gourgou

Chronis

2016

Sci Rep

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Oxidative stress (OS) impact on a single neuron’s function in vivo remains obscure. Using C. elegans as a model organism, we report the effect of paraquat (PQ)-induced OS on wild type worms on the function of the ASH polymodal neuron. By calcium (Ca2+) imaging, we quantified ASH activation upon stimulus delivery. PQ-treated worms displayed higher maximum depolarization (peak of the Ca2+ transients) compared to untreated animals. PQ had a similar effect on the ASH neuron response time (rising slope of the Ca2+ transients), except in very young worms. OS effect on ASH was partially abolished in vitamin C-treated worms. We performed octanol and osmotic avoidance tests, to investigate the OS effect on ASH-dependent behaviors. PQ-treated worms have enhanced avoidance behavior compared to untreated ones, suggesting that elevated ASH Ca2+ transients result in enhanced ASH-mediated behavior. The above findings suggest a possible hormetic effect of PQ, as a factor inducing mild oxidative stress. We also quantified locomotion parameters (velocity, bending amplitude), which are not mediated by ASH activation. Bending amplitude did not differ significantly between treated and untreated worms; velocity in older adults decreased. The differential effect of OS on behavioral patterns may mirror a selective impact on the organism’s neurons.

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“…Combined with fluorescence imaging of calcium transmitters and neurons, behavior mechanism could be explored in the neuronal level [60]. Based on the established microfluidic devices by several researchers, accurate stimuli could be delivered to individual worms flexibly and responses of worms behavior and neuron could be monitored [33,35,39,52,[61][62][63][64].…”

Section: Neuron Activitymentioning

confidence: 99%

Analysis of Caenorhabditis elegans in microfluidic devices

Wen

Qin

2012

Sci. China Chem.

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Caenorhabditis elegans (C. elegans) is widely adopted as a model organism for a variety of biological studies including development, genetics and neurobiology. Micro-scale microfluidic technology is capable of handling single or populations of C. elegans in high throughput format and allows for the precise spatial and temporal control of their environment, which is well suited for the study of worms in different aspects. In this review, we highlight the recent advances in microfluidic technology for the analysis of worms ranging from behavioral studies to neurobiology. We believe that microfluidic device can further be applied to study the different aspects of worms, extending from fundamental investigation of behavioral dynamics to more complicated biological processes including neurochemistry and learning behaviors. microfluidic, Caenorhabditis elegans, behavior, neurobiology, aging

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Probing the physiology of ASH neuron in Caenorhabditis elegans using electric current stimulation

Cited by 11 publications

References 20 publications

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Chemically induced oxidative stress affects ASH neuronal function and behavior in C. elegans

Analysis of Caenorhabditis elegans in microfluidic devices

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