Analysis of Caenorhabditis elegans in microfluidic devices

Wen, Hui; Qin, Jianhua

doi:10.1007/s11426-012-4541-x

Cited by 9 publications

(3 citation statements)

References 67 publications

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“…Recent advances in microfabrication technology and the adoption of microfluidics have made it possible to further investigate C. elegans phenotypic locomotive behaviour. 25,26 Several microfluidics-based C. elegans assays have been developed, which include mazes and arrays for learning and mobility behaviour, [27][28][29] clamps for immobilization and imaging, 30 an olfactory chip, 31 and a droplet-based system for individual movement assays. 32,33 Microfluidic devices have also been employed as research tools to study C. elegans locomotion patterns and behaviour.…”

Section: Introductionmentioning

confidence: 99%

On-chip analysis of C. elegans muscular forces and locomotion patterns in microstructured environments

et al. 2013

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Section: Introductionmentioning

confidence: 99%

On-chip analysis of C. elegans muscular forces and locomotion patterns in microstructured environments

et al. 2013

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“…Finally, C. elegans is small in size (1 to 1.3 mm), easy to culture, has a short developmental cycle (3 to 4 d) and life cycle (2 to 3 wk) [ 39 ], and thereby is inexpensive for large-volume studies [ 11 , 12 , 40 , 41 ]. The advantages of C. elegans spark many research interests and advances in biology, medicine, and engineering, among others [ 39 , 42 – 44 ].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic-Assisted Caenorhabditis elegans Sorting: Current Status and Future Prospects

Yuan

Duan

et al. 2023

Cyborg Bionic Syst

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Caenorhabditis elegans ( C. elegans ) has been a popular model organism for several decades since its first discovery of the huge research potential for modeling human diseases and genetics. Sorting is an important means of providing stage- or age-synchronized worm populations for many worm-based bioassays. However, conventional manual techniques for C. elegans sorting are tedious and inefficient, and commercial complex object parametric analyzer and sorter is too expensive and bulky for most laboratories. Recently, the development of lab-on-a-chip (microfluidics) technology has greatly facilitated C. elegans studies where large numbers of synchronized worm populations are required and advances of new designs, mechanisms, and automation algorithms. Most previous reviews have focused on the development of microfluidic devices but lacked the summaries and discussion of the biological research demands of C. elegans , and are hard to read for worm researchers. We aim to comprehensively review the up-to-date microfluidic-assisted C. elegans sorting developments from several angles to suit different background researchers, i.e., biologists and engineers. First, we highlighted the microfluidic C. elegans sorting devices' advantages and limitations compared to the conventional commercialized worm sorting tools. Second, to benefit the engineers, we reviewed the current devices from the perspectives of active or passive sorting, sorting strategies, target populations, and sorting criteria. Third, to benefit the biologists, we reviewed the contributions of sorting to biological research. We expect, by providing this comprehensive review, that each researcher from this multidisciplinary community can effectively find the needed information and, in turn, facilitate future research.

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“…Substantial advances in microfluidic techniques and particular research interest in C. elegans have driven the development of numerous microchip-based systems. They have been reviewed a number of times focusing on various aspects of miniaturized systems, their advantages, application challenges, and scientific potential [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. A summary of microfluidic-based systems with respect to the organism, organ, or tissue studies was presented by Sivagnanam et al [5].…”

Section: Introductionmentioning

confidence: 99%

Advanced Microfluidic Assays for Caenorhabditis elegans

Bakhtina¹,

MacKinnon²,

Korvink³

2016

Advances in Microfluidics - New Applications in Biology, Energy, and Materials Sciences

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The in vivo analysis of a model organism, such as the nematode Caenorhabditis elegans, enables fundamental biomedical studies, including development, genetics, and neurobiology. In recent years, microfluidics technology has emerged as an attractive and enabling tool for the study of the multicellular organism. "dvances in the application of microfluidics to C. elegans assays facilitate the manipulation of nematodes in high-throughput format and allow for the precise spatial and temporal control of their environment. In this chapter, we aim to illustrate the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans and discuss the trends of future development.Keywords: C. elegans, chip-based, manipulation, microfluidics, model organism . IntroductionThe invertebrate Caenorhabditis elegans, Drosophila melanogaster, and the vertebrate zebrafish Danio rerio are the most widely studied multicellular organisms. The in vivo analysis of these model organisms allows the understanding of many complex physiological processes, addressing many of the questions relevant to human biology. The choice of model organism depends on the biological question under investigation. For example, C. elegans is simple enough to be experimentally tractable. It has a short life cycle days at °C and lifespan days at °C , passes through four larva L L stages and an adult stage [ ]. Its small size . mm long and µm wide , transparent body at all life stages, and preferred food source Escherichia coli simplify its maintenance on agar plates or liquid cultures allowing visualization of individual cells and organs in intact animals. C. elegans possesses one of the simplest central nervous systems the adult hermaphrodite has neurons . "ecause it is so well studied, rapid identification of signaling pathways, for instance, in studies of aging, has © 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. become possible. "bout % of human disease genes have an orthologue in the genome of C. elegans, including those genes associated with "lzheimer disease, Parkinson's disease, Huntington's disease HD , and many other neurodegenerative disorders [ ]. This astonishing degree of correspondence permits the modeling of human ailments in a simple invertebrate without involving actual human subjects and provides a meaningful insight into the pathogenesis of a complex disease phenotype.Traditionally, behavioral genetics is employed as a prime method for neurobiological studies in C. elegans. It is based on manual worm manipulation on a Petri dish or a multiwell plate, and monitoring the effects on various biological processes, such as growth and fertility, by visual inspection. Refreshment of old buffer solutions by a fresh solution is invasive and causes stress both to the lar...

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Analysis of Caenorhabditis elegans in microfluidic devices

Cited by 9 publications

References 67 publications

On-chip analysis of C. elegans muscular forces and locomotion patterns in microstructured environments

On-chip analysis of C. elegans muscular forces and locomotion patterns in microstructured environments

Microfluidic-Assisted Caenorhabditis elegans Sorting: Current Status and Future Prospects

Advanced Microfluidic Assays for Caenorhabditis elegans

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