Probing the molecular regulation of lipopolysaccharide stress in piglet liver by comparative proteomics analysis

Abstract

Lipopolysaccharide (LPS) can induce inflammatory responses in piglets, causing immunological stress and tissue damage. However, chronic LPS infection may lead to LPS-induced immunological stress resistance. The molecular mechanisms underlying LPS stress have not been fully elucidated. Here, we conducted a global comparative proteomics analysis to investigate the molecular regulation of LPS stress using an immunological stress model of weaned piglets. A shotgun-based SWATH-MS workflow was used for global proteomes of the piglet livers after 15-day LPS treatment. Out of 3700 quantified proteins, 93 proteins showed differential changes under LPS stress. Bioinformatics analysis indicated that the differentially expressed proteins were mainly involved in inflammatory response, oxidation-redox processes and defense reactions, and were enriched in a phagosome pathway. Several key proteins associated with oxidative stress (SOD2), inflammation response (STEAP4 and S100 family) and the phagosome pathway were verified by activity and targeted-MS analyses. The observed responses appear to mitigate hepatic damage due to excessive oxidative stress, inflammation, and repression of the phagosome pathway. Our results reveal that an increased STEAP4 expression in piglets appears involved in cellular regulation by LPS stress and subsequent immunological stress resistance. This study sheds new light on the mechanism of prevention and relieving injury by LPS-induced immune responses.

Effects of Rhamnolipids on Growth Performance, Immune Function, and Cecal Microflora in Linnan Yellow Broilers Challenged with Lipopolysaccharides

“…Lipopolysaccharides (LPS), the essential outer membrane components of almost all Gram-negative bacteria, are known to stimulate immune responses to stresses in the host [ 16 ]. In the body, LPS might cause natural or innate immune responses, leading to elevation of inflammatory cytokines and a decrease in the immune function [ 17 , 18 ].…”

Dietary taurine supplementation attenuates lipopolysaccharide-induced inflammatory responses and oxidative stress of broiler chickens at an early age

“…Orally or abdominally injected LPS can increase the accumulation of proinflammatory mediators, leading to the production of cytokines and oxidative stress, which, in turn, causes hepatic inflammation (Hou et al, 2013;Wang et al, 2018). The LPS can trigger inflammatory responses in diverse species by activating signal pathways and promoting gene expressions, such as chickens (Chen et al, 2018), mouse (Zhang et al, 2020), and pig (Xia et al, 2018), which are characterized by reduced feed intake, decreased growth performance, and increased release of reactive oxygen species (ROS) and proinflammatory cytokine production (Takahashi et al, 2008;Munyaka et al, 2013;Gadde et al, 2017). It has been extensively used as a model to mimic bacterial infection and immunological stress in laboratory animals.…”

Effect of Dietary Amylose/amylopectin Ratio on Antioxidant Ability and Amino Acid Metabolism of Liver in Weaned Pigs Challenged with Lipopolysaccharide

“…We sample 12h after LPS challenge, 12-hour recovery time was more effective in fully restored piglet liver function than 2 hours. 15 day after LPS stress, proteins involved in in ammatory response, oxidation-redox processes and defense reactions were enriched in a phagosome pathway in liver of weaning piglet [4].…”