Probing the functional role of tick metalloproteases

Ali, Abid; Khan, Sardar; Ali, Ijaz; Karim, Shahid; Vaz, Itabajara da Silva; Termignoni, Carlos

doi:10.1111/phen.12104

Cited by 24 publications

(21 citation statements)

References 81 publications

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“…Metalloproteases were also among the identified enzymes with 9% from the set of S class proteins in both of the genders. These enzymes require a metal ion, usually Zn 2+ , to catalyze the hydrolysis of a peptide bond [52]. These proteolytic enzymes have been found throughout almost the entire evolutionary scale from bacteria to mammals [53, 54].…”

Section: Resultsmentioning

confidence: 99%

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

et al. 2019

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Background The hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. Results We reported the array of 1111 proteins identified in the salivary glands of H. dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. Conclusion This investigation represents the first proteomic study of H. dromedarii salivary glands. Our results shed light on the differences between the composition of H. dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully. Electronic supplementary material The online version of this article (10.1186/s12864-019-6042-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

et al. 2019

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“…While only 64 transcripts were overexpressed in H. dromedarii male SG, of which 41 had significant LFC, 81 transcripts were highly expressed in female ticks, of which 54 had a significant LFC. This protein family, which is commonly found in tick saliva, salivary glands, ovaries and the mid-gut, plays an important role in countering host inflammation, immunomodulation, fibrinolysis, blood protein digestion, nociception, vitellogenesis, remodeling the extracellular matrix and pathogen transmission [ 21 , 66 ]. Metalloproteases were characterized in several tick species: I. scapularis , A. americanum , Haemaphysalis longicornis , Rh.…”

Section: Resultsmentioning

confidence: 99%

De novo assembly and annotation of Hyalomma dromedarii tick (Acari: Ixodidae) sialotranscriptome with regard to gender differences in gene expression

et al. 2018

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BackgroundHard ticks are hematophagous ectoparasites characterized by their long-term feeding. The saliva that they secrete during their blood meal is their crucial weapon against host-defense systems including hemostasis, inflammation and immunity. The anti-hemostatic, anti-inflammatory and immune-modulatory activities carried out by tick saliva molecules warrant their pharmacological investigation. The Hyalomma dromedarii Koch, 1844 tick is a common parasite of camels and probably the best adapted to deserts of all hard ticks. Like other hard ticks, the salivary glands of this tick may provide a rich source of many compounds whose biological activities interact directly with host system pathways. Female H. dromedarii ticks feed longer than males, thereby taking in more blood. To investigate the differences in feeding behavior as reflected in salivary compounds, we performed de novo assembly and annotation of H. dromedarii sialotranscriptome paying particular attention to variations in gender gene expression.ResultsThe quality-filtered Illumina sequencing reads deriving from a cDNA library of salivary glands led to the assembly of 15,342 transcripts. We deduced that the secreted proteins included: metalloproteases, glycine-rich proteins, mucins, anticoagulants of the mandanin family and lipocalins, among others. Expression analysis revealed differences in the expression of transcripts between male and female H. dromedarii that might explain the blood-feeding strategies employed by both genders.ConclusionsThe annotated sialome of H. dromedarii helps understand the interaction of tick-host molecules during blood-feeding and can lead to the discovery of new pharmacologically active proteins of ticks of the genus Hyalomma.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2874-9) contains supplementary material, which is available to authorized users.

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“…Comparatively, it was evident from previous reports that outer membrane proteins are vaccine candidates and that cytoplasmic proteins are drug targets [ 21 , 109 ]. It is well known that exported proteins are the prominent molecules of interaction with cells infected by pathogens; therefore, they are potential candidates for vaccine targets [ 110 , 111 , 112 , 113 , 114 ]. The antigenicity and allergenicity analysis of target proteins revealed that eight among them were antigenic while the remaining four proteins were non-antigenic and all the target proteins were non-allergen ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Modeling Novel Putative Drugs and Vaccine Candidates against Tick-Borne Pathogens: A Subtractive Proteomics Approach

Ali

Ahmad

Wadood

et al. 2020

Veterinary Sciences

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Ticks and tick-borne pathogens (TBPs) continuously causing substantial losses to the public and veterinary health sectors. The identification of putative drug targets and vaccine candidates is crucial to control TBPs. No information has been recorded on designing novel drug targets and vaccine candidates based on proteins. Subtractive proteomics is an in silico approach that utilizes extensive screening for the identification of novel drug targets or vaccine candidates based on the determination of potential target proteins available in a pathogen proteome that may be used effectively to control diseases caused by these infectious agents. The present study aimed to investigate novel drug targets and vaccine candidates by utilizing subtractive proteomics to scan the available proteomes of TBPs and predict essential and non-host homologous proteins required for the survival of these diseases causing agents. Subtractive proteome analysis revealed a list of fifteen essential, non-host homologous, and unique metabolic proteins in the complete proteome of selected pathogens. Among these therapeutic target proteins, three were excluded due to the presence in host gut metagenome, eleven were found to be highly potential drug targets, while only one was found as a potential vaccine candidate against TBPs. The present study may provide a foundation to design potential drug targets and vaccine candidates for the effective control of infections caused by TBPs.

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Probing the functional role of tick metalloproteases

Cited by 24 publications

References 81 publications

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

De novo assembly and annotation of Hyalomma dromedarii tick (Acari: Ixodidae) sialotranscriptome with regard to gender differences in gene expression

Modeling Novel Putative Drugs and Vaccine Candidates against Tick-Borne Pathogens: A Subtractive Proteomics Approach

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