Probing the effects of microwave irradiation on enzyme-catalysed organic transformations: the case of lipase-catalysed transesterification reactions

Leadbeater, Nicholas E.; Stencel, Lauren M.; Wood, Eric C

doi:10.1039/b617544a

Cited by 47 publications

(31 citation statements)

References 25 publications

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“…A dose of 0.005% sodium azide was introduced to avoid any microbial contamination; and 1.0% (v/v) of Tween 80 was added to facilitate the enzymatic action. Seven separate samples were loaded with cellulase (5,10,15,20,25,30,35,FPU) …”

Section: Enzymatic Hydrolysis (Saccharification)mentioning

confidence: 99%

“…Microwave assisted enzymatic reactions or also called as Microwave Irradiation-Enzyme Coupling Catalysis (MIECC) reactions have been proven as a useful tool for many enzymatic transformations in both aqueous and organic solutions [30][31][32]. It has been proposed that in case of low power of high-frequency electromagnetic field the nonthermal activation of enzyme may be observed [33,34].…”

Section: Introductionmentioning

confidence: 99%

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Microwave-Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Saccharification of Oil Palm Empty Fruit Bunch Fiber for Enhanced Fermentable Sugar Yield

Nomanbhay¹,

Hussain²,

Palanisamy³

2013

JSBS

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Lignocellulosic materials are promising alternative feedstocks for bioethanol production. However, the recalcitrant nature of lignocellulosic biomass necessitates an efficient pretreatment pretreatment step to improve the yield of fermentable sugars and maximizing the enzymatic hydrolysis efficiency. Microwave pretreatment may be a good alternative as it can reduce the pretreatment time and improve the enzymatic activity during hydrolysis. The overall goal of this paper is to expand the current state of knowledge on microwave-based pretreatment of lignocellulosic biomass and microwave assisted enzymatic reaction or Microwave Irradiation-Enzyme Coupling Catalysis (MIECC). In the present study, a comparison of microwave assisted alkali pretreatment was tried using Oil Palm empty fruit bunch. The microwave assisted alkali pretreatment of EFB using NaOH, significantly improved the enzymatic saccharification of EFB by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes. The results showed that the optimum pretreatment condition was 3% (w/v) NaOH at 180 W for 12 minutes with the optimum component loss of lignin and holocellulose of about 74% and 24.5% respectively. The subsequent enzymatic saccharification of EFB pretreated by microwave assisted NaOH (3% w/v); resulted in 411 mg of reducing sugar per gram EFB at cellulose enzyme dosage of 20 FPU. The overall enhancement by the microwave treatment during the microwave assisted alkali pretreatment and microwave assisted enzymatic hydrolysis was 5.8 fold. The present study has highlighted the importance of well controlled microwave assisted enzymatic reaction to enhance the overall reaction rate of the process.

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Section: Enzymatic Hydrolysis (Saccharification)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Saccharification of Oil Palm Empty Fruit Bunch Fiber for Enhanced Fermentable Sugar Yield

Nomanbhay¹,

Hussain²,

Palanisamy³

2013

JSBS

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“…While microwaves have previously been utilized to enhance the rate, yield, and purity of organic reactions, they have more recently been employed in biological systems ranging from PCR [11,12] and DNA hybridizations [13] to the activation of enzymatic catalysis [14,15]. The oscillating electromagnetic wave is thought to directly interact with dipoles and ions to induce molecular motion, resulting in highly efficient heating.…”

Section: Introductionmentioning

confidence: 99%

Development of rapid microwave-mediated and low-temperature bacterial transformations

2013

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The introduction of exogenous DNA into Escherichia coli is a cornerstone of molecular biology. Herein, we investigate two new mechanisms for bacterial transformation involving either the use of microwave irradiation or a freeze-thaw protocol in liquid nitrogen. Ultimately, both methods afforded successful transfer of plasmid DNA into bacterial cells, with the freeze-thaw technique yielding efficiencies of~10 5 . More importantly, both techniques effectively eliminated the need for the preparation of competent cells.

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“…The moulds are strong amylolytic 27 . Microwave Irradiation-Enzyme Coupling Catalysis (MIECC) has also been proven as a useful tool for many enzymatic transformations in both aqueous and organic solutions [28][29][30] . It has been proposed that in case of low power of high-frequency electromagnetic field the nonthermal activation of enzyme may be observed 31,32 .…”

Section: 352mentioning

confidence: 99%

Microwave Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Hydrolysis of Palm Oil Mill Effluent (POME) for Optimum Fermentable Sugar Yield

2013

Chem Sci Trans

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Abstract:In the present study, a comparison of microwave assisted alkali pretreatment was tried using oil palm empty fruit bunch. The microwave assisted alkali pretreatment of POME using NaOH, significantly improved the enzymatic saccharification of POME by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes. Enzymatic hydrolysis of POME suspension by various amylolytic enzymes was investigated to reveal the potential coupling mechanism of Microwave Irradiation-Enzyme Coupling Catalysis (MIECC). It was shown that enzymatic hydrolysis of POME using typical enzymes may successfully was carried out at microwave condition. The MIECC resulted in increasing initial reaction rate by about 3 times. The results testify on specific activation of enzymes by microwaves and prove the existence of nonthermal effect in microwave assisted reactions. Low power microwave irradiation (100W) does not increase the temperature beyond 40 o C and hence denaturation of the enzyme is avoided. The present study has highlighted the importance of well controlled microwave assisted enzymatic reaction to enhance the overall reaction rate of the process.

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Probing the effects of microwave irradiation on enzyme-catalysed organic transformations: the case of lipase-catalysed transesterification reactions

Abstract: The lipase-catalysed transesterification reaction of methyl acetoacetate in toluene as a solvent has been studied using carefully controlled conditions. Results suggest that microwave heating does not have a noticeable effect on reaction rate or product conversion.

Cited by 47 publications

References 25 publications

Microwave-Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Saccharification of Oil Palm Empty Fruit Bunch Fiber for Enhanced Fermentable Sugar Yield

Microwave-Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Saccharification of Oil Palm Empty Fruit Bunch Fiber for Enhanced Fermentable Sugar Yield

Development of rapid microwave-mediated and low-temperature bacterial transformations

Microwave Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Hydrolysis of Palm Oil Mill Effluent (POME) for Optimum Fermentable Sugar Yield

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