Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘<i>Candidatus</i> Liberibacter asiaticus’ by <i>Diaphorina citri</i>

Silva, Marcus Vinicius Merfa E; Fischer, Eduarda Regina; Silva, Mariana de Souza e; Francisco, C. L.; Coletta-Filho, H. D.; Souza, Alessandra Alves de

doi:10.1094/phyto-06-21-0252-fi

Cited by 7 publications

(1 citation statement)

References 68 publications

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“…Identifying VmpA receptors could provide new insight in the mechanism of entry of the FD phytoplasma into insect cells. Moreover, in a context of pesticide reduction, this knowledge could help in developing new strategies of disease control by blocking the phytoplasma-insect cell interaction as it could be achieved for the phloem-sap pest "Candidatus Liberibacter asiaticus" (Merfa et al, 2022;Guo et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

Canuto,

Duret,

Dubrana

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionThe adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we identified Euscelidius variegatus cell proteins interacting with recombinant VmpA-His6.MethodsThe E. variegatus proteins were identified by mass spectrometry analysis of VmpA-E. variegatus protein complexes formed upon in vitro interaction assays. To assess their impact in VmpA binding, we reduced the expression of the candidate genes on E. variegatus cells in culture by dsRNA-mediated RNAi. The effect of candidate gene knockdown on VmpA binding was measured by the capacity of E. variegatus cells to bind VmpA-coated fluorescent beads.Results and discussionThere were 13 candidate proteins possessing potential N-glycosylation sites and predicted transmembrane domains selected. The decrease of expression of an unknown transmembrane protein with leucine-rich repeat domains (uk1_LRR) was correlated with the decreased adhesion of VmpA beads to E. variegatus cells. The uk1_LRR was more expressed in digestive tubes than salivary glands of E. variegatus. The protein uk1_LRR could be implicated in the binding with VmpA in the early stages of insect infection following phytoplasmas ingestion

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Section: Introductionmentioning

confidence: 99%

A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

Canuto,

Duret,

Dubrana

et al. 2023

Front. Cell. Infect. Microbiol.

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First report on the presence of huanglongbing vectors (Diaphorina citri and Trioza erytreae) in Ghana

Aidoo¹,

Ablormeti

Ninsin³

et al. 2023

Sci Rep

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As significant threats to global citrus production, Diaphorina citri (Kuwayama; Hemiptera: Psyllidae) and Trioza erytreae (Del Guercio; Hemiptera: Triozidae) have caused considerable losses to citrus trees globally. Diaphorina citri vectors “Candidatus Liberibacter asiaticus” and “Ca. L. americanus”, whereas T. erytreae transmits “Ca. L. africanus” and “Ca. L. asiaticus”, the pathogens responsible for citrus greening disease or Huanglongbing (HLB). Though HLB is a destructive disease of citrus wherever it occurs, information on the occurrence and geographical distribution of its vectors in Africa is limited. In recent surveys to determine if HLB vectors are present in Ghana, we observed eggs, nymphs, and adults of insects suspected to be D. citri and T. erytreae. Using morphological traits and DNA analyses, the identity of the suspected insects was confirmed to be D. citri and T. erytreae. Individuals of D. citri and T. erytreae were examined using qPCR for CLaf, CLam, and CLas, but none of them tested positive for any of the Liberibacter species. Herein we report, for the first time, the presence of D. citri and T. erytreae in Ghana (West Africa). We discuss the implications of this new threat to the citrus industry to formulate appropriate management strategies.

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Crossing the Gateless Barriers: Factors Involved in the Movement of Circulative Bacteria Within Their Insect Vectors

Sarkar¹,

Lin

Robledo³

et al. 2023

Phytopathology®

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Plant bacterial pathogens transmitted by Hemipteran vectors pose a large threat to the agricultural industry worldwide. While virus-vector relationships have been widely investigated, a significant gap exists in our understanding of the molecular interactions between circulative bacteria and their insect vectors, mainly leafhoppers and psyllids. In this review, we will describe how these bacterial pathogens adhere, invade, and proliferate inside their insect vectors. We will also highlight the different transmission routes and molecular factors of phloem-limited bacteria that maintain an effective relationship with the insect host. Understanding the pathogen-vector relationship at the molecular level will help in the management of vector-borne bacterial diseases.

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Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri

Cited by 7 publications

References 68 publications

A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

First report on the presence of huanglongbing vectors (Diaphorina citri and Trioza erytreae) in Ghana

Crossing the Gateless Barriers: Factors Involved in the Movement of Circulative Bacteria Within Their Insect Vectors

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