Probing protein adsorption onto polymer-stabilized silver nanocolloids towards a better understanding on the evolution and consequences of biomolecular coronas

Batista, Carin C. S.; Albuquerque, Lindomar J. C.; Jäger, Alessandro; Štěpánek, Petr; Giacomelli, Fernando C.

doi:10.1016/j.msec.2020.110850

Cited by 15 publications

(13 citation statements)

References 52 publications

(52 reference statements)

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“…PVA effectively stabilizes nanooxide AST 50 (Al 2 O 3 -SiO 2 -TiO 2 ) [10]. The capping with PVP remarkably improves the stability of hybrid AgNP in protein-containing environments especially in bovine serum albumin (BSA) solutions [11].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer films of polysaccharides modified with poly(N-vinylpyrrolidone) and poly(vinyl alcohol)

Livanovich¹,

Sharamet²,

Shimko³

et al. 2021

Heliyon

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N-grafted copolymers of chitosan (460 kDa) with poly(N-vinylpyrrolidone) (2.4 kDa) or poly(vinyl alcohol) (2.0 kDa) as side chains were synthesized. Depending on the polymer-to-chitosan mass ratio the degree of amino group substitution with side chains in chitosan backbone was varied in the range of 0.01-0.33. Layer-by-layer films consisted of copolymers and dextran sulfate as polyanion were obtained. Thickness, hydrophilicity, and morphology of the films were investigated using QCM, UV-vis spectrophotometry, AFM, and contact angle measurements. The obtained films show enhanced protein-repellent properties in fetal bovine serum medium. The mass of adsorbed proteins on LbL films based on copolymer with a degree of substitution of 0.2 decreases by 50 % compared to unmodified chitosan. Protein-repellent properties of copolymer-based films are common for LbL films of grafted chitosan copolymers and depend on hydrophilic side chain density on the surface.

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Section: Introductionmentioning

confidence: 99%

Layer-by-layer films of polysaccharides modified with poly(N-vinylpyrrolidone) and poly(vinyl alcohol)

Livanovich¹,

Sharamet²,

Shimko³

et al. 2021

Heliyon

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“…The authors investigated protein absorption on the Ag-NPs surface stabilized with a mixture of pVP, poly(ethylene oxide)—PEO, and PEO-b-P2VP (poly(ethylene oxide)-b-poly(2-vinyl pyridine). The results were satisfactory [ 68 ]. pVP was also used to coat other metal particles, such as gold [ 69 , 70 ].…”

Section: Pvp In Micro- and Nano Drug Delivery Systemsmentioning

confidence: 99%

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

2021

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Due to the unwanted toxic properties of some drugs, new efficient methods of protection of the organisms against that toxicity are required. New materials are synthesized to effectively disseminate the active substance without affecting the healthy cells. Thus far, a number of polymers have been applied to build novel drug delivery systems. One of interesting polymers for this purpose is povidone, pVP. Contrary to other polymeric materials, the synthesis of povidone nanoparticles can take place under various condition, due to good solubility of this polymer in several organic and inorganic solvents. Moreover, povidone is known as nontoxic, non-carcinogenic, and temperature-insensitive substance. Its flexible design and the presence of various functional groups allow connection with the hydrophobic and hydrophilic drugs. It is worth noting, that pVP is regarded as an ecofriendly substance. Despite wide application of pVP in medicine, it was not often selected for the production of drug carriers. This review article is focused on recent reports on the role povidone can play in micro- and nano drug delivery systems. Advantages and possible threats resulting from the use of povidone are indicated. Moreover, popular biomedical aspects are discussed.

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“…demonstrated that silica NPs coated with PEG at different MW form different PC when exposed to BSA, and that high MW PEG coatings are able to significantly suppress the formation of PC. [ 23 ] Other surface coatings and functionalizations that have been shown to affect BC‐related processes include glucose and polyethylene glycol, [ 78 ] polyvinylpyrrolidone (PVP) and poly(2‐vinyl pyridine)‐ b ‐poly(ethylene oxide) (PEO‐ b ‐P2VP), [ 79 ] zwitterionic moieties like cysteine, [ 27 ] a mixture of amine and zwitterionic moieties, [ 14 ] polyglycerol, [ 80 ] short‐chain ethylene oxide (EO) oligomers, [ 81 ] poly(phosphoester)s, [ 82 ] and starch. [ 83 ] Another BC influencing parameter that can be associated with the administration of nanostructures, but that is not an intrinsic property of NPs themselves, is the presence of stabilizing surfactants.…”

Section: Biomolecular Corona: Significance Characteristics and Exploi...mentioning

confidence: 99%

“…For example, for graphene oxide, silver nanostructures, gold nanostructures, and gold/silver nanoalloy, the incubation with proteins can change the peak of absorption corresponding to their surface plasmon resonance (SPR) wavelength, both in terms of intensity and wavelength. [ 5,28,79,132 ] As Dominguez‐Medina et al. note in their analysis of protein adsorption and subsequent unfolding on the surface of gold nanorods, the effect observed with the formation of BCs on nanostructures UV–vis absorption spectra may also be caused by an increase in the nanostructures aggregation levels.…”

Section: Techniques Used For Biomolecular Corona Analysismentioning

confidence: 99%

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

Battaglini

Gümüş

Ciofani

2021

Adv Materials Technologies

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investigating the use of nanomaterials within living organisms. When a nanoparticle (NP) encounters biological molecules such as proteins, lipids, nucleic acids, or polysaccharides, it tends to adsorb these molecules on its surface due to electrostatic, hydrophobic, or other forms of interactions between the molecules and the surface of the NP. [1][2][3][4] Depending on their abundance and affinity, proteins and other molecules may form a hard corona that consists of tightly bounded proteins on the surface, or a soft corona, which indicates a second layer of proteins that are loosely attached to the proteins of the hard corona. [4] The composition of the soft corona changes over time according to the environmental conditions, and so the biological identity of nanostructures is usually, but not always, determined by the hard corona. This can be both advantageous and disadvantageous to the application of NPs. Some studies have focused on preventing biomolecular corona (BC) formation, as this may change the size and surface properties, hinder modifications on the surface, and cause the rapid detection and clearance of NPs by the immune system. [5,6] However, BCs can be exploited or even altered, in order to be made beneficial for targeting purposes as they can enable specific cells to more easily recognize NPs and may lead to a reduction in their toxicity. [7,8] A wide range of techniques have been used in BC studies, such as dynamic light scattering (DLS), [9] sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry (MS)-based approaches, [10][11][12][13][14][15][16][17][18][19][20][21] UV/vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), [22] atomic force microscopy (AFM), [23] scanning electron microscopy (SEM), [24][25][26][27] transmission electronic microscopy (TEM), [23] and circular dichroism spectroscopy (CD). [28] Various information (size, surface properties, morphology, structure, etc.) about BCs can be obtained through these methods. Many techniques have been applied in BC studies, which can be divided into microscopybased approaches that use direct imaging of the sample, and indirect techniques. Microscopy techniques can provide information about corona composition and shape with a spatial resolution up to the dimension scale of single protein molecules, but they are limited by the requirement for staining or extensive sample preparation. Indirect techniques can provide extensive information about BC-nanostructure interactions and properties, but typically have a limited resolution and so cannot analyze the BC at the level of single nanostructures. Other emerging

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Probing protein adsorption onto polymer-stabilized silver nanocolloids towards a better understanding on the evolution and consequences of biomolecular coronas

Cited by 15 publications

References 52 publications

Layer-by-layer films of polysaccharides modified with poly(N-vinylpyrrolidone) and poly(vinyl alcohol)

Layer-by-layer films of polysaccharides modified with poly(N-vinylpyrrolidone) and poly(vinyl alcohol)

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

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