Probing Mechanical Properties of Jurkat Cells under the Effect of ART Using Oscillating Optical Tweezers

Khakshour, Samaneh; Beischlag, Timothy V.; Sparrey, Carolyn J.; Park, Edward J.

doi:10.1371/journal.pone.0126548

Cited by 12 publications

(15 citation statements)

References 23 publications

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“…The measured bead displacements for each group of cells after exposure to 72 h of hypoxia or normoxia, along with the solution of the bead motion equation were used to identify viscoelastic properties of the cells 26 . The viscoelastic properties of the cell include shear modulus and viscosity coefficient.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, inhibition of either the ERK1/2 or AKT-MTOR pathways prevented actin reorganization that may contribute to cancer cell transformation and motility. In order to analyze alterations in the mechanical properties of control and Rb-knockdown LNCaP cells, resultant bead movements were measured under hypoxic and normoxic conditions using an oscillating OT 26 . The results show time dependent changes in bead movement for cells (shSCX and shRb) cultured under hypoxia for 72 h. In addition, increased cell migration in hypoxic shRb LNCaP cells suggests that there is a correlation between increased cell migration and actin reorganization.…”

Section: Discussionmentioning

confidence: 99%

“…The stiffness of shRNA LNCaP cells was measured using an oscillating OT as described previously 26 . Briefly, the OT experimental set up is composed of a 3 W continuous wave, Nd:YAG laser beam with a wavelength of 1064 nm.…”

Section: Methodsmentioning

confidence: 99%

“…In order to analyze the experimental data and calculate the changes in cell mechanical properties, a bead motion equation was used as described previously 26 . The cell relaxation time was calculated by measuring fluctuations in the position of trapped beads linked to the cell cytoskeleton.…”

Section: Methodsmentioning

confidence: 99%

“…The oscillating OT is an instrument that uses a highly focused laser beam to trap and oscillate a microbead attached to the cell cytoskeleton and therefore, exert a quantifiable force on the cell. The technical details of our setup have been described previously 26 . In summary, a continuous wave 3 W, Nd:YAG laser emitting light at a wavelength of 1064 nm was used with a Nikon TE2000 inverted microscope.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Retinoblastoma protein (Rb) links hypoxia to altered mechanical properties in cancer cells as measured by an optical tweezer

Khakshour

Labrecque

Esmaeilsabzali

et al. 2017

Sci Rep

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Hypoxia modulates actin organization via multiple pathways. Analyzing the effect of hypoxia on the biophysical properties of cancer cells is beneficial for studying modulatory signalling pathways by quantifying cytoskeleton rearrangements. We have characterized the biophysical properties of human LNCaP prostate cancer cells that occur in response to loss of the retinoblastoma protein (Rb) under hypoxic stress using an oscillating optical tweezer. Hypoxia and Rb-loss increased cell stiffness in a fashion that was dependent on activation of the extracellular signal-regulated kinase (ERK) and the protein kinase B (AKT)- mammalian target of rapamycin (MTOR) pathways. Pharmacological inhibition of MEK1/2, AKT or MTOR impeded hypoxia-inducible changes in the actin cytoskeleton and inhibited cell migration in Rb-deficient cells conditioned with hypoxia. These results suggest that loss of Rb in transformed hypoxic cancer cells affects MEK1/2-ERK/AKT-MTOR signalling and promotes motility. Thus, the mechanical characterization of cancer cells using an optical tweezer provides an additional technique for cancer diagnosis/prognosis and evaluating therapeutic performance.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Retinoblastoma protein (Rb) links hypoxia to altered mechanical properties in cancer cells as measured by an optical tweezer

Khakshour

Labrecque

Esmaeilsabzali

et al. 2017

Sci Rep

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Mechanically Defined Microgels by Droplet Microfluidics

Heida

Neubauer

Seuß

et al. 2016

Macro Chemistry & Physics

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Over the last two decades, droplet‐based microfluidics has evolved into a versatile tool for fabricating tailored micrometer‐sized hydrogel particles. Combining precise fluid handling down to femtoliter scale with diverse hydrogel precursor design, it allows for excellent control over microgel size and shape, but also functionalization and crosslinking density. Consequently, it is possible to tune physicochemical and mechanical properties such as swelling, degradation, stimuli sensitivity, and elasticity by microfluidic droplet templates. This has led to a recent trend in applying microgels as experimental platform in cell culturing, drug delivery, sensing, and tissue engineering. This article highlights advances in microfluidic droplet formation as templates for microgels with tailored physicochemical properties. Special focus is put on evolving design strategies for the synthesis of mechanically defined microgels, their applications, and methods for mechanical characterization on single‐particle level.

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The effects of paranodal myelin damage on action potential depend on axonal structure

Kohan

Lashkari

Sparrey

2017

Med Biol Eng Comput

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Biophysical computational models of axons provide an important tool for quantifying the effects of injury and disease on signal conduction characteristics. Several studies have used generic models to study the average behavior of healthy and injured axons; however, few studies have included the effects of normal structural variation on the simulated axon's response to injury. The effects of variations in physiological characteristics on axonal function were mapped by altering the structure of the nodal, paranodal, and juxtaparanodal regions across reported values in three different caliber axons (1, 2, and 5.7 μm). Myelin detachment and retraction were simulated to quantify the effects of each injury mechanism on signal conduction. Conduction velocity was most affected by axonal fiber diameter (89%), while membrane potential amplitude was most affected by nodal length (86%) in healthy axons. Postinjury axonal functionality was most affected by myelin detachment in the paranodal and juxtaparanodal regions when retraction and detachment were modeled simultaneously. The efficacy of simulated potassium channel blockers on restoring membrane potential and velocity varied with axonal caliber and injury type. The structural characteristics of axons affect their functional response to myelin retraction and detachment and their subsequent response to potassium channel blocker treatment.

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Probing Mechanical Properties of Jurkat Cells under the Effect of ART Using Oscillating Optical Tweezers

Cited by 12 publications

References 23 publications

Retinoblastoma protein (Rb) links hypoxia to altered mechanical properties in cancer cells as measured by an optical tweezer

Retinoblastoma protein (Rb) links hypoxia to altered mechanical properties in cancer cells as measured by an optical tweezer

Mechanically Defined Microgels by Droplet Microfluidics

The effects of paranodal myelin damage on action potential depend on axonal structure

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