Background: Hemodialysis is a process in renal replacement function. Comorbid is one of the contributing factors for the high mortality among hemodialysis patients. Objective: To determine the correlation between comorbidities and duration of HD treatment and the effect of HD treatment duration on mortality rates in chronic kidney disease mortality patients undergoing hemodialysis. Methods: A retrospective cohort study was conducted in this study. This study included Hemodialysis patients with comorbid HD patients (DM, Hypertension and Congestive Heart disease) who died in 2018. The HD population was 560 patients (n=84) using convenience sampling method. The analysis was carried out using the Chi Square test and the Kruskall Wallis and Mann-Whitney test. Certificate of Ethical Eligibility from the Health Research Ethics Committee of RSUP DR R.D Kandou Manado no: 064/EC/KEPK-KANDAU/VII/2020. Results: The results of treatment were of 0-24 months (40.5 %), 25-48 months (33.3%) and > 48 months (26.2 %). The analysis showed a significant correlation between the duration of HD treatment and comorbidities in CKD patients undergoing HD, namely comorbid DM after HD (p=0.002), hypertension after HD (p=0.010), congestive heart failure after HD (p=0.003), with 2 comorbidities (p=0.048), and 3 comorbidities (p=0.011). Besides, the effect of the duration of HD treatment on the mortality rate is known that the duration of treatment >48 months gives a lower mortality rate with an average treatment time of 360 days. Conclusion: HD patients with the duration of treatment of 0-12 months died the most than the duration of treatment >25-48 months. There was a significant correlation between Comorbid-3 and the duration of HD treatment in CKD patients (p=0.011).