Pro-oxidant status based alterations in cerebellar antioxidant response to aluminum insult

Nayak, Prasunpriya; Sharma, Shiv Bhushan; Chowdary, N V S

doi:10.1134/s1819712412010114

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…Neurons are particularly sensitive to oxidative stress and their functional terms depend on the oxidant status of the local microenvironment [6,7]. On the other hand, because of their unsaturated side chain, penetration into brain tissues is easier for TT [8].…”

Section: Introductionmentioning

confidence: 99%

Effect of Tocotrienol Pretreatment on Ex Vivo Superoxide and Peroxide Handling Capacities (Sphc) of Rat Serum and Brain

Dasari

Anandamurali

Nayak

2017

Int J Pharm Pharm Sci

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Objective: Tocotrienol (TT), a constituent of vitamin E, present only in selected seed oil. Because of the isoprenoid side chain, antioxidant property of tocotrienol is recently highlighted. Application of tocotrienol is also proven to be neuroprotective. The current study was aimed to evaluate the effect of tocotrienol pretreatment on the serum and brain oxidative stress parameters and oxidant handling capacities. Methods:Male albino Wistar rats were treated with tocotrienol (10 mg/day) for two weeks and maintained for the next four weeks. Levels of reduced glutathione and lipid peroxidation and activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were estimated fortnightly in serum. After sacrifice, oxidative stress parameters were measured in the frontal cortex, temporal cortex, thalamic area, hippocampus and cerebellum. Glutathione-dependent and glutathione-independent superoxide and peroxide handling capacities (SPHC) were calculated for serum and brain regions. Data collected from both the groups are statistically processed with Kruskal-Wallis test and Mann-Whitney pairwise comparisons.Results: Significant impacts of TT treatment have been observed in terms of growth and water intake. Serum SPHC (Glutathione-independent) has been found to be reduced significantly immediately after the TT treatment. Region-specific alterations in oxidative stress parameters have also been observed after 4 w of supplementation. Global reductions in reduced glutathione and lipid peroxidation have been observed in the brain without any alteration in the SPHC. Conclusion:From the results, it can be suggested that the tocotrienol pretreatment possibly be used as neuroprotective measure particularly against oxidative stress. In addition, the antioxidant impacts of TT were found to be maintained for a longer period in brain regions, even though it was not so in the case of serum.

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Section: Introductionmentioning

confidence: 99%

Effect of Tocotrienol Pretreatment on Ex Vivo Superoxide and Peroxide Handling Capacities (Sphc) of Rat Serum and Brain

Dasari

Anandamurali

Nayak

2017

Int J Pharm Pharm Sci

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“…[51] Different modalities Et exposure were reported earlier. [26,37] Increased production of hydroxyl radical and levels of lipid peroxidation were observed in Et-exposed cerebellar granule cell in vitro, [31] indicating direct contribution of Et exposure on the oxidative stress. Interestingly, almost a dose-dependent increase in cerebellar LPO levels was observed in NT3 group of the current study, however, without no substantial alteration in cerebellar GSH level.…”

Section: Discussionmentioning

confidence: 99%

“…Even in chronic exposure, Et was ineffective to alter the brain weight while body weights were significantly less compared to control animals. [25] Comparison of Et-induced neuronal damage in different brain regions were reviewed in Nayak et al [26] which indicated the possibility of neurodegenerative impact on cerebellum even in moderate doses of Et exposure and demonstrated the significant alterations in cerebellar oxidative stress parameters with high dose of Et exposure. In the current study, only insignificant influences of Et exposure were observed in cerebellar GSH content, SOD activity and catalase activity.…”

Section: Biochemical Parametermentioning

confidence: 99%

Evaluation of Varied Modalities of Tocotrienol Supplementations to Counter the Cerebellar Oxidative Stress Caused by Low-to-moderate Doses of Ethanol in Rats

Dasari¹,

Nayak²

2019

IJCEP

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Background and Aim: Self-intoxication with Ethanol (Et) is a common problem worldwide. Being a psychoactive drug, neurotoxic effects of Et are well known. Cerebellum is highly vulnerable to Et exposure. Tocotrienols (T3) are relatively rare components of vitamin E and have the potential to prevent the oxidative stress and act as neuroprotective. Varied modalities of T3 supplementations were evaluated to identify the possibilities of countering cerebellar oxidative stress caused by low-to-moderate doses of Et exposure. Methods: Four phase of experiments were carried out with nil (Et-0) and three doses of Et exposures (Et-I, Et-II and Et-III) for 4 weeks. In each phase, 4 groups of Wistar rats were maintained with sham supplementation (NT3), Prior Supplementation (PT3), Simultaneous Supplementation (ST3) and Total Supplementation (TT3) with T3 for 6 weeks. Cerebellar levels of reduced Glutathione (GSH), Lipid Peroxidation (LPO) and activities of catalase, Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) and Glutathione Reductase (GR) were estimated and Superoxide and Peroxide Handling Capacities (SPHCs) were calculated. Results: All the tested cerebellar oxidative stress parameters and handling capacities were significantly influenced by the modalities of T3 supplementation. However, low-to-moderate doses of Et exposures contributed significantly in alterations of LPO level, GPx activity, GR activity and glutathione-dependent SPHC of cerebellum. Conclusion: Critical evaluation of studied parameters suggest insufficient overall performance of ST3 type of supplementation, whereas, TT3 type of supplementation was the best among the modalities of T3 supplementation. However, excess T3 supplementation may not be beneficial in some cases of oxidative stress parameters and SPHC of cerebellum.

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Alpha-Tocopherol Supplementation Restricts Aluminium- and Ethanol-Induced Oxidative Damage in Rat Brain but Fails to Protect Against Neurobehavioral Damage

Nayak

Sharma

Chowdary³

2018

Journal of Dietary Supplements

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The concurrent presence of oxidative stress (OS) and aluminium exposure is an inducer of neurodegenerative changes. Aluminium can augment OS in a pro-oxidant dominant condition. Antioxidative property of α-tocopherol may be useful in restricting these degenerative changes in the brain. OS parameters are tested in frontal cortex (FC), hippocampus (HC), and cerebellum (CL) of α-tocopherol-supplemented (5 IU/day) male Wistar rats exposed to aluminium (10 mg Al/Kg/day; "Al"), ethanol (0.6 g ethanol/Kg/day; "Et"), and both ("Al-Et") and vehicle-treated control ("C") for 4 weeks. The α-tocopherol supplementation restricted regional alterations of reduced glutathione, superoxide dismutase, catalase, and glutathione peroxidase. Accordingly, the regional superoxide and peroxide handling capacities (SPHC) also remain unaltered. Al-Et group demonstrated significant elevation in the lipid peroxidation level in FC and CL regions compared to the group C; similar elevations in lipid peroxidation were noted in all the tested brain regions of Al group. Likewise, declines in glutathione reductase activity were noted in HC (versus Et group) and CL (versus Al and Et groups) of Al-Et group. Interestingly, changes in behavioral patterns of all the treatment groups are comparable while differing from that of the control group. Significant difference with group C is observed during first through fourth weeks, third to fourth weeks, and second to third weeks in terms of spontaneous motor activity, Rota Rod performance, and Hebb-Williams maze performance, respectively. Hence, the current dose and duration of α-tocopherol supplementation failed to provide full protection against the aluminium-induced neurodegeneration; nevertheless, it could provide only partial protection toward aluminium-induced augmentation of OS in specific brain regions.

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Pro-oxidant status based alterations in cerebellar antioxidant response to aluminum insult

Cited by 3 publications

References 39 publications

Effect of Tocotrienol Pretreatment on Ex Vivo Superoxide and Peroxide Handling Capacities (Sphc) of Rat Serum and Brain

Effect of Tocotrienol Pretreatment on Ex Vivo Superoxide and Peroxide Handling Capacities (Sphc) of Rat Serum and Brain

Evaluation of Varied Modalities of Tocotrienol Supplementations to Counter the Cerebellar Oxidative Stress Caused by Low-to-moderate Doses of Ethanol in Rats

Alpha-Tocopherol Supplementation Restricts Aluminium- and Ethanol-Induced Oxidative Damage in Rat Brain but Fails to Protect Against Neurobehavioral Damage

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