Effect of Tocotrienol Pretreatment on Ex Vivo Superoxide and Peroxide Handling Capacities (Sphc) of Rat Serum and Brain

Dasari, Pitchaiah; Anandamurali, R; Nayak, Prasunpriya

doi:10.22159/ijpps.2017v9i3.15866

Cited by 7 publications

(8 citation statements)

References 18 publications

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“…The larvae fed on diet supplemented with AB when allowed to develop into adults maintained on normal diet also show a decrease in total GSH content as compared to control flies, throughout the span of 40 days. It has been reported that increase in GSH content leads to a proportionate increase in antioxidant capacity of an organism [29][30][31]. In the current study, it has been noticed that even with low concentration of GSH, the intrinsic GSH:GSSG ratio remains unaltered in flies from AB-supplemented regimes.…”

Section: Discussionsupporting

confidence: 46%

Modulation of Oxidative Stress by Abhrak Bhasma in Drosophila Melanogaster

Subedi¹,

Vartak

Kale³

2018

Asian J Pharm Clin Res

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Objective: Management of aging is one of the most actively researched areas in biology. Ayurvedic preparations such as Abhrak Bhasma (AB) have been used for improving longevity and regenerative therapy as well as in treating various ailments. Modes of action of such Ayurvedic preparations are poorly understood at molecular level. Current investigation tests the efficacy of AB in modulating various parameters linked to oxidative stress.Methods: Drosophila melanogaster was used as a model system to test the efficacy of AB in inducing antioxidant machinery. Drosophila flies were fed on diet supplemented with AB at larval and adult stages. Subsequently, various parameters, catalytic as well as non-catalytic, related to antioxidant machinery were analyzed.Results: AB has been found to modulate the activity of superoxide dismutase and catalase enzymes as well as the total reduced glutathione (GSH) content. Larvae and adults feeding on diet supplemented with AB exhibit significantly lower levels of total GSH content (decrease of about 40-70% for larvae, while 31-36% for adults) and without any conclusive effect on GSH:oxidized glutathione ratio, free radical scavenging capacity, and extent of lipid peroxidation. These larvae and adults fed on diet supplemented with AB also exhibited an increase in the level of transcription of cap "n" collar C, heat shock protein 70, and catalase genes (≥1.5 fold, except in few cases). Conclusion:Overall, AB alters various parameters linked to antioxidant machinery in D. melanogaster. The induced components may provide protection to the organism during stressful conditions.

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Section: Discussionsupporting

confidence: 46%

Modulation of Oxidative Stress by Abhrak Bhasma in Drosophila Melanogaster

Subedi¹,

Vartak

Kale³

2018

Asian J Pharm Clin Res

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“…This was in accordance with our earlier observation where the same dose of T3 showed significant changes in body weight. [23] The percentage changes in body weight, in the current study, were also influenced significantly by the Et exposures in all the groups except PT3. Therefore, even in the absence of overall change in absolute body weight, the currently used low-to-moderate doses of Et exposure was sufficient to contribute significantly in the slowing of body weight gain.…”

Section: Discussionsupporting

confidence: 57%

“…Maintenance of systemic, organ or regional GSH level by vitamin E, TRF or T3 in presence of oxidative stressor had been reported times and again. [23,32,[37][38][39][40][41][42] Increases in cerebellar GSH level were noticed in the ST3 group (statistically significant) and TT3 group (statistically significant) in comparison to NT3 and PT3 groups of Et-0 phase of current study. However, in presence of Et exposures, TT3 group abled to maintain the level of cerebellar GSH while ST3 group failed to do so.…”

Section: Biochemical Parametermentioning

confidence: 51%

Evaluation of Varied Modalities of Tocotrienol Supplementations to Counter the Cerebellar Oxidative Stress Caused by Low-to-moderate Doses of Ethanol in Rats

Dasari¹,

Nayak²

2019

IJCEP

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Background and Aim: Self-intoxication with Ethanol (Et) is a common problem worldwide. Being a psychoactive drug, neurotoxic effects of Et are well known. Cerebellum is highly vulnerable to Et exposure. Tocotrienols (T3) are relatively rare components of vitamin E and have the potential to prevent the oxidative stress and act as neuroprotective. Varied modalities of T3 supplementations were evaluated to identify the possibilities of countering cerebellar oxidative stress caused by low-to-moderate doses of Et exposure. Methods: Four phase of experiments were carried out with nil (Et-0) and three doses of Et exposures (Et-I, Et-II and Et-III) for 4 weeks. In each phase, 4 groups of Wistar rats were maintained with sham supplementation (NT3), Prior Supplementation (PT3), Simultaneous Supplementation (ST3) and Total Supplementation (TT3) with T3 for 6 weeks. Cerebellar levels of reduced Glutathione (GSH), Lipid Peroxidation (LPO) and activities of catalase, Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) and Glutathione Reductase (GR) were estimated and Superoxide and Peroxide Handling Capacities (SPHCs) were calculated. Results: All the tested cerebellar oxidative stress parameters and handling capacities were significantly influenced by the modalities of T3 supplementation. However, low-to-moderate doses of Et exposures contributed significantly in alterations of LPO level, GPx activity, GR activity and glutathione-dependent SPHC of cerebellum. Conclusion: Critical evaluation of studied parameters suggest insufficient overall performance of ST3 type of supplementation, whereas, TT3 type of supplementation was the best among the modalities of T3 supplementation. However, excess T3 supplementation may not be beneficial in some cases of oxidative stress parameters and SPHC of cerebellum.

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“…A high intake of antioxidants may decrease the risk of colon cancer and prevent the metastases [1][2][3]. One of the antioxidant sources naturally abundant is tocopherol and tocotrienol or Vitamin E which is soluble in water [4]. However, Vitamin E degrades easily by chemical or physical action during storage or when it is delivered to the appropriate site of action after ingestion [5].…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Vitamin E Onto Cross-Linked Galactomannan Phosphate Matrix and in Vitro Study

Tarigan

Purba

Zuhra

2018

Asian J Pharm Clin Res

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Objective: This study demonstrated the incorporation of Vitamin E from palm fatty acid distillate onto crosslinked galactomannan phosphate (CGP) matrix.Methods: CGP was obtained from the crosslinking reaction of galactomannan from Arenga pinnata (GAP) with tri-sodium metaphosphate (TMF) ranging from 1:1 to 4:3 while incorporation of Vitamin E was conducted in two steps to form films. The reliability study of Vitamin E in CGPVE was conducted using a solution of pepsin and sodium chloride and also in solution of pancreatin and buffer phosphate. Results:The Fourier-transform infrared spectrum indicated the presence of phosphate in CGP while the scanning electron microscope images depicted the changes of surface morphology from smooth (GAP) to rough and hollow (CGP) which confirmed that crosslink had occurred. The swelling study of CGP showed that the swelling indexes were similar and decreased with the increase of TMF. The efficiency of CGP to absorb Vitamin E ranged from 89.66% to 91.09%. The in vitro releasing study of Vitamin E in simulated gastric fluid and simulated intestinal fluid showed that only a small amount of Vitamin E was released.Conclusions: This study demonstrated that CGP can be prepared and is potentially useful for drug delivery to the colon.

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Effect of Tocotrienol Pretreatment on Ex Vivo Superoxide and Peroxide Handling Capacities (Sphc) of Rat Serum and Brain

Cited by 7 publications

References 18 publications

Modulation of Oxidative Stress by Abhrak Bhasma in Drosophila Melanogaster

Modulation of Oxidative Stress by Abhrak Bhasma in Drosophila Melanogaster

Evaluation of Varied Modalities of Tocotrienol Supplementations to Counter the Cerebellar Oxidative Stress Caused by Low-to-moderate Doses of Ethanol in Rats

Incorporation of Vitamin E Onto Cross-Linked Galactomannan Phosphate Matrix and in Vitro Study

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