Pro-inflammatory cytokine responses in human gingival epithelial cells after stimulation with cell wall extract of Aggregatibacter actinomycetemcomitans subtypes

Pahumunto, Nuntiya; Chotjumlong, Pareena; Makeudom, Anupong; Krisanaprakornkit, Suttichai; Dahlén, Gunnar; Teanpaisan, Rawee

doi:10.1016/j.anaerobe.2017.08.001

Cited by 16 publications

(29 citation statements)

References 21 publications

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“…Lipopolysaccharide (LPS) [9] and leukotoxin [14] of A. actinomycetemcomitans are immunodominant antigens that stimulate immune cell produced pro-inflammatory cytokines and chemokine, which is in accordance with this study. A. actinomycetemcomitans CWEs and supernatant showed significantly 7 higher cytotoxicity and IL-1β secretion by PBMC cells compared to lactobacilli.…”

Section: Discussionsupporting

confidence: 87%

“…Similarly, the effect on IL-1β release was more pronounced for the JP2 clone, indicating the effect on host cells may be specifically associated with leukotoxin production and with a potential costimulation of PBMC´s by lipopolysaccharides (LPS). A. actinomycetemcomitans is a bacterial species associated with periodontitis due to its leukotoxin production, internalization into fibroblast cells, and immunomodulation [8,9]. In a previous study it was shown that A. actinomycetemcomitans suppressed IL-8 mRNA expression and its function [9] as well as, this study revealed the potency of A.…”

Section: Discussionsupporting

confidence: 58%

“…A standard curve was prepared by PBMCs added to 96-well plates at 2 x 10 6 cells/well and cultured in the presence of the various concentrations (50, 100, 200, 400, 800, 1000 µg/ml) of lactobacilli CWEs and tested for cytotoxicity and cytokine secretion. A concentration of 100 µg/ml of lactobacilli CWEs was used while the concentration of A. actinomycetemcomitans CWEs was obtained from our previous study [9]. For the supernatant of both strains, an undiluted supernatant was used throughout this study.…”

Section: Bacterial Exposurementioning

confidence: 99%

“…This genotype, termed the JP2 clone, primarily found in West African populations, has been closely associated with periodontitis in young individuals [6,7]. Previous studies on various subtypes of A. actinomycetemcomitans strains isolated from an adult Thai population with periodontitis, revealed a significant variation in IL-8 cytokine expression (but not IL-1β, IL-6, and TNF-α) in human gingival epithelial cells (HGECs) [8,9]. Non-serotypable strains (NS1 and NS2) and a JP2-like strain showed significantly lower IL-8 responses than the serotypable (serotype a-f) stains [9].…”

Section: Introductionmentioning

confidence: 99%

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Oral lactobacilli reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro.

Pahumunto

Basic

Östberg

et al. 2020

Preprint

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Background: This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMC) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomycetemcomitans strains, representing different subtypes, and three Lactobacillus strains were used. The PBMC cells from six blood donors were exposed to supernatants and CWEs of A. actinomycetemcomitans or Lactobacillus strains alone or combinations and untreated cells as control.The cytotoxicity was determined by trypan blue exclusion-method and IL-1β secretion by ELISA. TNFα, IL-6 and IL-8 secretions were measured using Bioplex Multiplex Immunoassay.Results: Supernatants or CWEs from all bacterial strains showed cytotoxicity and IL-1β secretion and the subtypes of A. actinomycetemcomitans showed generally a significantly higher effect on PBMC´s than that of the Lactobacillus strains. Two highly toxic A. actinomycetemcomitans strains (JP2 and JP2like) induced a higher response than all other strains. Two non-serotypable strains (NS1 and NS2) showed a weaker although significant effect than other A. actinomycetemcomitans or Lactobacillus strains tested. CWEs showed generally a higher effect on PBMC´s than the supernatants. When combined, Lactobacillus significantly reduced the toxicity and IL-1β secretion induced by A.acinomycetemcomitans. The effect varied between the subtypes and the reduction was highest for the JP2 and JP2-like strains. The Lactobacillus paracasei strain SD1 had a higher reducing effect that the other Lactobacillus strains. This strain had a consistent reducing effect on all subtypes of A. actinomycetemcomitans cytotoxicity, and release of IL-1β and TNF-α from PBMC´s of the blood donors. A strong and significant variation in cytokine release between the 6 blood donors was noticed.Conclusions: Lactobacillus spp and L. paracasei strain SD1 in particular, showed a significant reducing interaction with A. actinomycetemcomitans toxicity and release of cytokines in vitro. 13Special thanks for technical assistances in the

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 58%

Section: Bacterial Exposurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oral lactobacilli reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro.

Pahumunto

Basic

Östberg

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…This genotype, termed the JP2 clone, primarily found in North and West African populations, has been closely associated with periodontitis in young individuals [6,7]. Previous studies on various subtypes of A. actinomycetemcomitans strains isolated from an adult Thai population with periodontitis, revealed a signi cant variation in IL-8 cytokine expression (but not IL-1β, IL-6, and TNFα) in human gingival epithelial cells (HGECs) [8,9]. Non-serotypable strains (NS1 and NS2) and a JP2-like strain showed signi cantly lower IL-8 responses than the serotypable (serotype a-f) stains [9].…”

Section: Introductionmentioning

confidence: 99%

Oral Lactobacillus strains reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro.

Pahumunto

Basic

Östberg

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMCs) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomycetemcomitans strains, representing different subtypes, and three Lactobacillus strains were used. The PBMCs from six blood donors were exposed to supernatants and CWEs of A. actinomycetemcomitans or Lactobacillus strains alone or combinations and untreated cells as control. The cytotoxicity was determined by trypan blue exclusion method and IL-1β secretion by ELISA. TNF-α, IL-6, and IL-8 secretions were measured using Bioplex Multiplex Immunoassay. Results: Supernatants or CWEs from all bacterial strains showed cytotoxicity and IL-1β secretion and the subtypes of A. actinomycetemcomitans showed generally a significantly higher effect on PBMCs than that of the Lactobacillus strains. Two highly toxic A. actinomycetemcomitans strains (JP2 and JP2-like) induced a higher response than all other strains. When combined, Lactobacillus significantly reduced the toxicity and the IL-1β secretion induced by A. acinomycetemcomitans . The effect varied between the subtypes and the reduction was highest for the JP2 and JP2-like strains. The Lactobacillus paracasei strain SD1 had a higher reducing effect than the other Lactobacillus strains. This strain had a consistent reducing effect on all subtypes of A. actinomycetemcomitans cytotoxicity, and release of IL-1β , IL-6, IL-8, and TNF-α from PBMCs of the blood donors. A strong and significant variation in cytokine release between the six blood donors was noticed. Conclusions: Lactobacillus spp. and L. paracasei SD1 in particular, showed a limited but statistically significant reducing interaction with A. actinomycetemcomitans toxicity and release of cytokines in vitro .

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Anti-cancer Properties of Potential Probiotics and Their Cell-free Supernatants for the Prevention of Colorectal Cancer: an In Vitro Study

Pahumunto

Teanpaisan

2022

Probiotics & Antimicro. Prot.

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Pro-inflammatory cytokine responses in human gingival epithelial cells after stimulation with cell wall extract of Aggregatibacter actinomycetemcomitans subtypes

Cited by 16 publications

References 21 publications

Oral lactobacilli reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro.

Oral lactobacilli reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro.

Oral Lactobacillus strains reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro.

Anti-cancer Properties of Potential Probiotics and Their Cell-free Supernatants for the Prevention of Colorectal Cancer: an In Vitro Study

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