Pro-angiogenic Molecules for Therapeutic Angiogenesis

Selvaprithviraj, Vignesh; Sankar, Deepthi; Amirthalingam, Sivashanmugam; Srinivasan, Sowmya; Rangasamy, Jayakumar

doi:10.2174/0929867324666170724142641

Cited by 15 publications

(9 citation statements)

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“…In addition to growth factors and cytokines, electrospun nanofibers have been utilized to load and control the release of angiogenic small molecules (e.g., angiogenin) and phytochemicals (e.g., curcumin) in order to accelerate the healing process [38,39]. However, it should be highlighted that the angiogenic efficacy of such substances is strongly dose-dependent [40,41]. It is worth mentioning that surface functionalization of nanofibrous scaffolds by angiogenic bioactive molecules was also proven as a feasible approach for promoting tissue repair and regeneration [42,43].…”

Section: Fiber Orientationmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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Angiogenesis (or the development of new blood vessels) is a key event in tissue engineering and regenerative medicine; thus, a number of biomaterials have been developed and combined with stem cells and/or bioactive molecules to produce three-dimensional (3D) pro-angiogenic constructs. Among the various biomaterials, electrospun nanofibrous scaffolds offer great opportunities for pro-angiogenic approaches in tissue repair and regeneration. Nanofibers made of natural and synthetic polymers are often used to incorporate bioactive components (e.g., bioactive glasses (BGs)) and load biomolecules (e.g., vascular endothelial growth factor (VEGF)) that exert pro-angiogenic activity. Furthermore, seeding of specific types of stem cells (e.g., endothelial progenitor cells) onto nanofibrous scaffolds is considered as a valuable alternative for inducing angiogenesis. The effectiveness of these strategies has been extensively examined both in vitro and in vivo and the outcomes have shown promise in the reconstruction of hard and soft tissues (mainly bone and skin, respectively). However, the translational of electrospun scaffolds with pro-angiogenic molecules or cells is only at its beginning, requiring more research to prove their usefulness in the repair and regeneration of other highly-vascularized vital tissues and organs. This review will cover the latest progress in designing and developing pro-angiogenic electrospun nanofibers and evaluate their usefulness in a tissue engineering and regenerative medicine setting.

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Section: Fiber Orientationmentioning

confidence: 99%

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

et al. 2020

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“…Use of angiosuppressive compounds to inhibit neovascularization can potentially prevent or reduce such proliferations, thus the risk of cancer . Meanwhile, pro‐angiogenesis treatment is gaining attention, not just for wound healing but also for tissue and organ regeneration, especially for damaged or abnormal cardiovascular system and age‐related ischemia …”

Section: Resultsmentioning

confidence: 99%

“…[53][54][55] Meanwhile, pro-angiogenesis treatment is gaining attention, not just for wound healing but also for tissue and organ regeneration, especially for damaged or abnormal cardiovascular system and age-related ischemia. [56][57][58][59]…”

Section: Anti-angiogenic Activitymentioning

confidence: 99%

Enhanced bioactivity and efficient delivery of quercetin through nanoliposomal encapsulation using rice bran phospholipids

et al. 2018

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BACKGROUND Quercetin is a phenolic compound occurring in many food plants and agricultural crops. It is reported to possess various health‐promoting properties. However, the poor bioavailability of quercetin, due to its low aqueous solubility and its degradation during digestion, limits its nutraceutical applications. This study aimed to encapsulate quercetin in nanoliposomes using rice‐bran phospholipids for its efficient delivery and controlled release, the protection of its structural stability, and enhancement of its bioactivity. RESULTS Nanoliposomal encapsulation of quercetin by thin film‐sonication method yielded spherical nanoparticles (157.33 ± 23.78 nm) with high encapsulation efficiency (84.92 ± 0.78%). Storage stability studies showed that nanoliposomal quercetin was stable at 4 °C and 27 °C for 6 and 5 months, respectively, as indicated by unchanged antioxidant activity and quercetin retention. Nanoliposomal quercetin showed a slow, limited release pattern in simulated gastric fluid (SGF), and an initial burst release followed by a slow constant releasing pattern in simulated intestinal fluid (SIF). A 1004‐fold increase in 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical‐scavenging activity was observed in quercetin nanoliposomes (SC50 = 4.04 ± 0.01 ppm) compared to non‐encapsulated quercetin (SC50 = 4053.03 ± 5.61 ppm). Similarly, the anti‐angiogenic activity of quercetin, as evaluated by duck embryo chorioallantoic membrane (CAM) assay, was enhanced twofold to fivefold by nanoliposomal encapsulation. CONCLUSION This study showed that nanoliposomal encapsulation in rice‐bran phospholipids enhanced the radical‐scavenging and anti‐angiogenic activities of quercetin. Furthermore, this study demonstrated that nanoliposomes can serve as efficient oral delivery system for quercetin. © 2018 Society of Chemical Industry

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“…Histological analysis of ischemic skin showed that tissues from the CD34+ Group contained more CD31+ cells than those from the ADSC Group. ADSC have been reported to have angiogenic effects by secreting VEGF, bFGF and other growth factors [29,30] . In our ex vivo study, we found that adipose-derived CD34+ cells expressed higher levels of VEGF and bFGF mRNA than ADSCs, as well as the related secreted proteins.…”

Section: Discussionmentioning

confidence: 99%

Superiority of Adipose-derived CD34+ Cells over Adipose-derived Stem Cells in Promoting Ischamic Tissue Survival

Liu¹,

Zhang

Tan³

et al. 2021

Preprint

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Background: Tissue ischemia usually leads to necrosis and is a threatening condition associated with reconstructive surgery. Promoting the survival of ischemic tissue is critical for improving clinical outcomes. Although various solutions based on stem cells have been reported, there are still limitations to clinical translation. The aim of this study was to develop an effective method to promote the survival of ischemic tissue. Methods: Adipose-derived CD34+ and CD34- cells were obtained by magnetic bead sorting from the stromal vascular faction (SVF). Adipose-derived stem cell (ADSC) were collected by subculture. The angiogenic capacities of CD34+ cells, CD34- cells and ADSC were evaluated in vitro by comparing mRNA and protein expression. Random axial flaps in nude mice were used to evaluate the efficacy of these cells in protecting tissue from necrosis. The effect of these cells in preventing inflammation was also evaluated. Results: Our data suggest that CD34+ cells expressed higher levels of angiogenetic factors and lower levels of inflammatory factors than the other cell types. More vessel branches were formed when human umbilical vein endothelial cells (HUVECs) were treated with conditioned medium from CD34+ cells than conditioned medium from the other cell types. Compared to ADSC, CD34+ cells showed significantly higher efficacy in promoting tissue survival. More CD31+ cells and higher levels of angiogenic factors were observed in tissues from the CD34+ Group than from the other Groups. Lower levels of the proinflammatory factors TNF-α and IL-1b and higher levels of anti-inflammatory factors were found in the CD34+ Group than in the other Groups.Conclusion: Adipose-derived CD34+ cells showed better efficacy in improving ischemic tissue survival than ADSC by reducing tissue inflammation and promoting angiogenesis. CD34+ cells can be obtained easily and may be suitable for clinical applications.

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Pro-angiogenic Molecules for Therapeutic Angiogenesis

Cited by 15 publications

References 0 publications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Electrospun Nanofibers for Improved Angiogenesis: Promises for Tissue Engineering Applications

Enhanced bioactivity and efficient delivery of quercetin through nanoliposomal encapsulation using rice bran phospholipids

Superiority of Adipose-derived CD34+ Cells over Adipose-derived Stem Cells in Promoting Ischamic Tissue Survival

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