Printed Graphene Electrochemical Biosensors Fabricated by Inkjet Maskless Lithography for Rapid and Sensitive Detection of Organophosphates

Hondred, John A.; Breger, Joyce C.; Alves, Nathan J.; Trammell, Scott A.; Walper, Scott A.; Medintz, Igor L.; Claussen, Jonathan C.

doi:10.1021/acsami.7b19763

Cited by 116 publications

(83 citation statements)

References 67 publications

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“…5,27 This facile LIG manufacturing protocol also eliminates the need for ink preparation, ink printing, and post-print annealing processes associated with solution-phase printed graphene. 9,[28][29] The utility of LIG has been displayed in numerous applications including supercapacitors, 14,22,[30][31] non-biofouling surfaces, 32 transparent heaters 33 , and more recently, electrochemical sensors. [25][26][34][35] For example, a non-enzymatic amperometric glucose sensor comprised of LIG functionalized with Cu nanocubes was used to selectively measure glucose over a wide concentration range (25 µM-4 mM), which is physiologically relevant to glucose levels in saliva, tears, and blood.…”

mentioning

confidence: 99%

Flexible Laser-Induced Graphene for Nitrogen Sensing in Soil

Garland¹,

McLamore

Cavallaro

et al. 2018

ACS Appl. Mater. Interfaces

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129

107

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Flexible graphene electronics are rapidly gaining interest, but their widespread implementation has been impeded by challenges with ink preparation, ink printing, and post-print annealing processes. Laser-induced graphene (LIG) promises a facile alternative by creating flexible graphene electronics on polyimide substrates through a one-step laser writing fabrication method. Herein we demonstrate the use of LIG, created through a low-cost UV laser, for electrochemical ion selective sensing of plant-available nitrogen (i.e., both ammonium and nitrate ions: NH4+ and NO3-) in soil samples. The laser used to create the LIG was operated at distinct pulse rates (10, 20, 30, 40, and 50 ms) in order to maximize the LIG electrochemical reactivity. Results illustrated that a laser pulse rate of 20 ms led to a high percentage of sp2 carbon (77%) and optimal peak oxidation current of 120 uA during ferricyanide cyclic voltammetry. Therefore, LIG electrodes created with a 20 ms pulse rate were consequently functionalized with distinct ionophores specific to NH4+(nonactin) or NO3-(tridodecylmethylammonium nitrate) within polyvinyl chloride (PVC)-based membranes to create distinct solid contact ion selective electrodes (SC-ISEs) for NH4+ and NO3-ion sensing respectively. The LIG SC-ISEs displayed near Nernstian sensitivities of 51.7 ± 7.8 mV/decade (NH4+) and -54.8 ± 2.5 mV/ decade (NO3-), detection limits of 28.2 ± 25.0 uM (NH4+) and 20.6 ± 14.8 uM (NO3-), low long-term drift of 0.93 mV/hr (NH4+) sensors and -5.3 μV/hr (NO3-) sensors and linear sensing ranges within 10-5-10-1 M for both sensors. Moreover, soil slurry sensing was performed and recovery percentages of 96% and 95% were obtained for added NH4+and NO3-, respectively. These results, combined with a facile fabrication that does not require metallic nanoparticle decoration, make these LIG electrochemical sensors appealing for a wide range of in field or point-of-service applications for soil health management. ABSTRACTFlexible graphene electronics are rapidly gaining interest, but their widespread implementation has been impeded by challenges with ink preparation, ink printing, and postprint annealing processes. Laser-induced graphene (LIG) promises a facile alternative by creating flexible graphene electronics on polyimide substrates through a one-step laser writing fabrication method. Herein we demonstrate the use of LIG, created through a low-cost UV laser, for electrochemical ion selective sensing of plant-available nitrogen (i.e., both ammonium and nitrate ions: NH 4 + and NO 3 -metallic nanoparticle decoration, make these LIG electrochemical sensors appealing for a wide range of in-field or point-of-service applications for soil health management.

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mentioning

confidence: 99%

Flexible Laser-Induced Graphene for Nitrogen Sensing in Soil

Garland¹,

McLamore

Cavallaro

et al. 2018

ACS Appl. Mater. Interfaces

Self Cite

129

107

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“…In the high concentration range (0.8−5 pmol L −1 ), the regression equation was i pc = −0.0049[paraoxon] −13.615 ( R 2 = 0.9954). Meanwhile, the LOD of Nafion/M‐Cell/CNT@AAIL/GCE biosensor was calculated to be 3 fmol L −1 (S/N = 3), which was 2–8 orders of magnitude lower than those of the various reported paraoxon assay methods, including spectrophotometry, fluorescence, electrochemistry, flow‐injection amperometric assay, and other methods (Table S1, Supporting Information). Therefore, the as‐fabricated biosensor showed ultrasensitivity and short detection time, which was more suitable for on‐site detection of trace level of OPP.…”

Section: Resultsmentioning

confidence: 96%

“…In addition, many electrochemical OPP sensors were applied at positive potential, based on oxidation currents of analytes, where some antioxidants in real samples can also be oxidized. More importantly, the limit‐of‐detection (LOD) values of the reported OPP analytic methods (containing electrochemical and non‐electrochemical methods) generally are within 0.12 pmol L −1 to 200 nmol L −1 . Nevertheless, the OPP residues of persisting in crops and environment may be lower .…”

Section: Introductionmentioning

confidence: 99%

Rational Integration of Biomineralization, Microbial Surface Display, and Carbon Nanocomposites: Ultrasensitive and Selective Biosensor for Traces of Pesticides

Han

Chen

2018

Adv Materials Inter

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real-time detection, simple operation, high sensitivity, and miniaturization. [12,13] So far, the most of electrochemical OPP biosensors are based on the inhibiting effect of enzymes, particularly acetylcholinesterase (AChE). [14][15][16][17][18][19][20] However, the poor specificity of AChE and enzyme inhibition-based sensing mechanism can make electrochemical biosensors to have low selectivity, complicated analysis process, low sensitivity, and slow response, restricting their application. In addition, many electrochemical OPP sensors were applied at positive potential, based on oxidation currents of analytes, [21,22] where some antioxidants in real samples can also be oxidized. More importantly, the limit-of-detection (LOD) values of the reported OPP analytic methods (containing electrochemical and non-electrochemical methods) generally are within 0.12 pmol L −1 to 200 nmol L −1 . [5,[21][22][23][24][25][26][27][28][29][30][31][32][33][34] Nevertheless, the OPP residues of persisting in crops and environment may be lower. [4,5] Therefore, it is of great significance to fabricate ultrasensitive and efficient OPP biosensor.To overcome these above problems, organophosphate hydrolase (OPH) is introduced into electrochemical OPP biosensors to substitute enzyme inhibition-based biosensors. [35] OPH can specifically hydrolyze organophosphate compounds, where OPP are the substrate of the enzyme rather than the inhibitor. [1,36] Hence, OPH-based analytical methods generally show good selectivity and simple assay procedure. However, there are relatively few reports about the electrochemical biosensor based on OPH, compared with other biosensors. [27,37] In previous work, we prepared OPH-fused cell-cobalt phosphate (CP) bioinorganic hybrid materials, that is, mineralized cell (M-Cell), for enhancing the activity of OPH by the integration of allosteric effect, [38,39] biomineralization technology, [40] and cell surface display technology. [16] However, concomitantly, inorganic salt (CP) and whole-cell can reduce the conductivity and electrochemical activity of M-Cell hybrid catalysts. Therefore, it is interesting and necessary to improve the electrochemical activity of M-Cell hybrid catalysts in order to establish the electrochemical OPP biosensor with ultrasensitivity.As is well known, carbon nanotube (CNT) is extensively used to construct electrochemical biosensors, due to the large specific surface area, good stability, and high conductivity. [41] However, Nowadays, the over misuse of organophosphate pesticides (OPP) has seriously threatened human health and environment, and it is challenging and indispensable to establish biosensor for supersensitive, reliable, and fast detection of traces of OPP in real samples. This work provides an interdisciplinary strategy for the construction of electrochemical OPP biosensor with supersensitivity, high selectivity, simple operation, and fast response. First, amino acid ionic liquid (AAIL) is used as stabilizer to dramatically improve the dispersibility of carbon nanotube (CNT) i...

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“…In one example, phosphotriesterase (PTE) was conjugated to the graphene surface via glutaraldehyde cross-linking and, as a result, rapid sensing with a response time of 5 s and an LOD of 3 nM was obtained. (95,96) It was hypothesized that the high sensitivity was achieved by the preparation of this heterogenous electrode with the assistance of laser annealing, which optimized the morphology of graphene as a template for biofunctionalization. Very detailed information on the preparation of this graphene electrode for this biosensor is described in Ref.…”

Section: Detection Of Chemical Warfare Agent Simulantmentioning

confidence: 99%

“…Very detailed information on the preparation of this graphene electrode for this biosensor is described in Ref. 95.…”

Section: Detection Of Chemical Warfare Agent Simulantmentioning

confidence: 99%

Graphene-based Materials in Gas Sensor Applications: A Review

Demon¹,

Kamisan²,

Abdullah³

et al. 2020

Sensors and Materials

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Graphene and chemically modified graphene can be fabricated via numerous routes each with its own merits concerning ease of processability, cost-effectiveness for large-scale production, and also health and safety. One of the promising applications of graphene-based composites is gas sensing, which is mainly useful for environmental monitoring. We review some of the significant findings on graphene-based sensing materials for the detection of organic vapors, toxic gases, and chemical warfare agent simulants using an electrochemical method. Electrochemical sensing can be performed by inducing interactions between gas molecules and a graphene layer, such as charge transfer that gives a change in an electrical signal. The intrinsic properties of graphene and its role in some gas sensing applications will be discussed. Graphene and graphene oxide (GO) work as continuous conductive networks with a large number of surface adsorption sites for many gas molecules. Hybrid graphene devices incorporate semiconductors, metals, and molecular binders to enhance the capabilities of solidstate gas sensors. This article also addresses current approaches to the commercialization of graphene-based gas sensors.

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Printed Graphene Electrochemical Biosensors Fabricated by Inkjet Maskless Lithography for Rapid and Sensitive Detection of Organophosphates

Cited by 116 publications

References 67 publications

Flexible Laser-Induced Graphene for Nitrogen Sensing in Soil

Flexible Laser-Induced Graphene for Nitrogen Sensing in Soil

Rational Integration of Biomineralization, Microbial Surface Display, and Carbon Nanocomposites: Ultrasensitive and Selective Biosensor for Traces of Pesticides

Graphene-based Materials in Gas Sensor Applications: A Review

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