Printed 384‐Well Microtiter Plate on Paper for Fluorescent Chemosensor Arrays in Food Analysis

Abstract

We propose a printed 384-well microtiter paperbased fluorescent chemosensor array device (384-well microtiter PCAD) to simultaneously categorize and discriminate saccharides and sulfur-containing amino acids for food analysis. The 384-well microtiter PCAD requiring 1 μL/4 mm 2 of each well can allow high-throughput sensing. The device embedded with self-assembled fluorescence chemosensors displayed a fingerprint-like response pattern for targets, the image of which was rapidly captured by a portable digital camera. Indeed, the paper-based chemosensor array system combined with imaging analysis and pattern recognition techniques not only successfully categorized saccharides and sulfur-containing amino acids but also classified mono-and disaccharide groups. Furthermore, the quantitative detectability of the printed device was revealed by a spike and recovery test for fructose and glutathione in a diluted freshly made tomato juice. We believe that the 384-well microtiter PCAD using the imaging analysis system will be a powerful sensor for multi-analytes at several categorized groups in real samples.

Solid-state optical chemosensor array devices for real-sample analysis

“…Very recently, the author proposed a printed 384-well microtiter paper-based fluorescent chemosensor array device (384well microtiter PCSAD) to simultaneously categorize and discriminate saccharides and sulfur-containing amino acids that play roles as not only essential taste components but also indicators to monitor food freshness (Figure 2(a),(b)). 7 Remarkably, the 384-well microtiter PCSAD requiring 1 μL/4 mm 2 of each well can be fabricated by using a common office printer, which could promote the establishment of easy-to-obtain chemical sensors in real-world scenarios. Certainly, the paper-based chemosensor array system combined with imaging analysis and pattern recognition techniques not only successfully categorized saccharides and sulfur-containing amino acids but also classified monoand disaccharide groups (Figure 2(c)).…”

“…With the combination of pattern recognition techniques, chemosensor arrays possessing cross-reactivities are capable of simultaneously discriminating multiple analytes. [1][2][3] By the employment of appropriate solid support materials (e.g., polymer gels 4 and paper substrates [5][6][7] ), fingerprint-like response patterns on the solid-state chemosensor array devices can be rapidly recorded by portable digital recorders, followed by imaging analysis (Figure 1). Indeed, the author first succeeded in the qualitative and quantitative detection of various types of chemical species in real samples by using optimized paper-based microtiter plates.…”

“…Indeed, the author first succeeded in the qualitative and quantitative detection of various types of chemical species in real samples by using optimized paper-based microtiter plates. [5][6][7] Figure 1. Illustrated conceptual figure of solid-state optical chemosensor array devices for pattern recognition.…”

Printed colorimetric chemosensor array on a 96-microwell paper substrate for metal ions in river water

“…Molecular recognition information on chemosensor arrays in solution is conventionally acquired by spectrophotometers, whereas the applicability to on-site analysis is limited by solution-based sensing systems (Sasaki et al, 2021b;Sasaki et al, 2022). Hence, we have focused on chemosensor arrays embedded in solid-state sensor devices to establish easy-to-use chemical sensor devices (Xu and Bonizzoni, 2020;Lyu et al, 2021;Zhang et al, 2021;Lyu et al, 2022). Paper is a representative solid-state substrate in the field of analytical chemistry, and its superior processability has facilitated the development of printed paper-based analytical devices (Martinez et al, 2010;Feng et al, 2013;Yamada et al, 2015;Karita and Kaneta, 2016;Tan et al, 2022).…”