Printable gelatin, alginate and boron nitride nanotubes hydrogel-based ink for 3D bioprinting and tissue engineering applications

Kakarla, Akesh Babu; Kong, Ing; Turek, Ilona; Kong, Cin; Irving, Helen Ruth

doi:10.1016/j.matdes.2021.110362

Cited by 37 publications

(22 citation statements)

References 81 publications

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“…According to previous report [48], the alginate, gelatin, and BNNTs hydrogel composite scaffolds were produced. Briefly, alginate (5 w/v%) and gelatin (6 w/v%) were mixed in deionised water through constantly stirring for 1 h. Later, BNNTs (1 w/v%) was slowly added into the alginate and gelatin solution and stirred for another 1 h at 60 • C. The obtained solution was loaded into a 3-mL syringe with attached 22-gauge nozzle.…”

Section: Methodsmentioning

confidence: 99%

Mechanical Behaviour Evaluation of Porous Scaffold for Tissue-Engineering Applications Using Finite Element Analysis

et al. 2022

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In recent years, finite element analysis (FEA) models of different porous scaffold shapes consisting of various materials have been developed to predict the mechanical behaviour of the scaffolds and to address the initial goals of 3D printing. Although mechanical properties of polymeric porous scaffolds are determined through FEA, studies on the polymer nanocomposite porous scaffolds are limited. In this paper, FEA with the integration of material designer and representative volume elements (RVE) was carried out on a 3D scaffold model to determine the mechanical properties of boron nitride nanotubes (BNNTs)-reinforced gelatin (G) and alginate (A) hydrogel. The maximum stress regions were predicted by FEA stress distribution. Furthermore, the analysed material model and the boundary conditions showed minor deviation (4%) compared to experimental results. It was noted that the stress regions are detected at the zone close to the pore areas. These results indicated that the model used in this work could be beneficial in FEA studies on 3D-printed porous structures for tissue engineering applications.

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Section: Methodsmentioning

confidence: 99%

Mechanical Behaviour Evaluation of Porous Scaffold for Tissue-Engineering Applications Using Finite Element Analysis

et al. 2022

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“…The most dominant cell types used in the included articles were human embryonic kidney cells (HEK293, HEK293T) [24,25,74,81,154], human neuroblastoma cells (SH-SY5Y) [30,64,68,73,76,79,82,155], mouse embryonic fibroblasts (NIH/3T3) [70], human dermal fibroblasts (HDF) [31,36,48,51,55], lung fibroblasts (MRC-5) [65,156], dental pulp fibroblasts [34,41,62], lung epithelium cells (A549) [37,55,74], macrophage cells (RAW 264.7) [49,74], human liver epithelium (HeP G2) [27,28,37], human peripheral blood (NB4) [37], glioblastoma cells (malignant U87 [30,37,156], T98 [80,156]), mammary gland adenocarcinoma cells (MCF-7) [156], N9 murine microglia cells [50], osteoblasts [54,66,75,77], osteoblast precursor cell line derived from mouse musculus calvaria (M3CT3) [47]<...…”

Section: Cell Sourcesmentioning

confidence: 99%

“…Another very simple method is Trypan Blue assay. Trypan Blue highlights the dead cells that are easily observed under bright-field microscopy [24,25,82].…”

Section: Cell Viability Assaysmentioning

confidence: 99%

“…In biomedical and tissue-engineering applications, when BNNTs are functionalised with various organic and inorganic materials, non-toxicity is reported, up to dosage levels of 100 µ g•mL −1 . Thus far, some of the studies have suggested applications of BNNTs in cancer-tumour treatment [32,61,80], drug carries or drug delivery [30], radioisotope accumulation of tumours [31], MRI contrast agents [68,73], reinforcement for biomaterials to produce tissue scaffolds [53,56], orthopaedic procedures [75,77], dental procedures [41,62], bioimaging [158], and bioprinting [24,25]. Not only are there biocompatibility properties but there are also amazing piezoelectrical properties, leading some to propose BNNTs as nanotransducers for the electrical stimulation of cells [39,56].…”

Section: Biomedical and Tissue-engineering Applicationsmentioning

confidence: 99%

“…Similarly, BNNTs combined with resin-based dental sealants showed potential materials in therapeutic procedures of dental hard tissues [34]. In another study, Kakarla et al demonstrated that BNNTs reinforced gelatine and alginate as a hydrogel to produce hydrogel scaffolds for tissue engineering applications [24,25].…”

Section: Tissue Engineeringmentioning

confidence: 99%

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In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review

Kakarla

Kong

2022

Nanomaterials

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Boron nitride nanotubes (BNNTs) are an exciting class of nanomaterials due to their unique chemical and physical characteristics. In recent decades, BNNTs have gained huge attention in research and development for various applications, including as nano-fillers for composites, semiconductor devices, hydrogen storage, and as an emerging material in biomedical and tissue engineering applications. However, the toxicity of BNNTs is not clear, and the biocompatibility is not proven yet. In this review, the role of BNNTs in biocompatibility studies is assessed in terms of their characteristics: cell viability, proliferation, therapeutic outcomes, and genotoxicity, which are vital elements for their prospective use in biomedical applications. A systematic review was conducted utilising the databases Scopus and Web of Science (WOS) (2008–2022). Additional findings were discovered manually by snowballing the reference lists of appropriate reviews. Only English-language articles were included. Finally, the significant analysis and discussion of the chosen articles are presented.

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Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

et al. 2023

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Advances in bioprinting have enabled the fabrication of complex tissue constructs with high speed and resolution. However, there remains significant structural and biological complexity within tissues that bioprinting is unable to recapitulate. Bone, for example, has a hierarchical organization ranging from the molecular to whole organ level. Current bioprinting techniques and the materials employed have imposed limits on the scale, speed, and resolution that can be achieved, rendering the technique unable to reproduce the structural hierarchies and cell–matrix interactions that are observed in bone. The shift towards biomimetic approaches in bone tissue engineering, where hydrogels provide biophysical and biochemical cues to encapsulated cells, is a promising approach to enhance the biological function and development of tissues for in vitro modelling. A major focus in bioprinting of bone tissue for in vitro modelling is creating the dynamic microenvironmental niches to support, stimulate, and direct the cellular processes for bone formation and remodeling. Hydrogels are ideal materials for imitating the extracellular matrix since they can be engineered to present various cues whilst allowing bioprinting. Here, we review recent advances in hydrogels and 3D bioprinting towards creating a microenvironmental niche that is conducive to tissue engineering of in vitro models of bone.This review focuses on hydrogels and 3D bioprinting in bone tissue engineering for development of in vitro models of bone. It highlights challenges in recapitulating the biological complexity seen in bone and how synergistic application of dynamic hydrogels and innovative bioprinting pipelines could address these challenges to achieve bone models.This article is protected by copyright. All rights reserved

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Printable gelatin, alginate and boron nitride nanotubes hydrogel-based ink for 3D bioprinting and tissue engineering applications

Cited by 37 publications

References 81 publications

Mechanical Behaviour Evaluation of Porous Scaffold for Tissue-Engineering Applications Using Finite Element Analysis

Mechanical Behaviour Evaluation of Porous Scaffold for Tissue-Engineering Applications Using Finite Element Analysis

In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

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