‘Printability' of Candidate Biomaterials for Extrusion Based 3D Printing: State‐of‐the‐Art

Kyle, Stuart; Jessop, Zita M.; Al‐Sabah, Ayesha; Whitaker, Iain S.

doi:10.1002/adhm.201700264

Cited by 347 publications

(284 citation statements)

References 114 publications

(61 reference statements)

Supporting

Mentioning

281

Contrasting

Order By: Relevance

“…[25][26][27][28][29] CNC can be mixed into various polymer matrices to reinforce the mechanical strength and induce shear thinning behavior. 28,30,31 For example, Siqueira et al developed a hybrid ink comprising of CNC, 2-hydroxyethyl methacrylate (HEMA) monomer and polyether urethane acrylate (PUA). They found that the CNC particles align along the printing direction by shear flow, and the CNC inks display shear-thinning behavior.…”

Section: -13mentioning

confidence: 99%

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Lin²,

et al. 2018

View full text Add to dashboard Cite

Abstract3D bioprinting is a novel platform for engineering complex, three-dimensional (3D) tissues that mimic real ones. The development of hybrid bioinks is a viable strategy that integrates the desirable properties of the constituents. In this work, we present a hybrid bioink composed of alginate and cellulose 2 nanocrystals (CNCs) and explore its suitability for extrusion-based bioprinting. This bioink possesses excellent shear-thinning property, can be easily extruded through the nozzle, and provides good initial shape fidelity. It has been demonstrated that the viscosities during extrusion were at least two orders of magnitude lower than those at small shear rates, enabling the bioinks to be extruded through the nozzle (100 μm inner diameter) readily without clogging. This bioink was then used to print a liver-mimetic honeycomb 3D structure containing fibroblast and hepatoma cells. The structures were crosslinked with CaCl 2 and incubated and cultured for 3 days. It was found that the bioprinting process resulted in minimal cell damage making the alginate/CNC hybrid bioink an attractive bioprinting material.Graphical abstract

show abstract

Section: -13mentioning

confidence: 99%

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Lin²,

et al. 2018

View full text Add to dashboard Cite

show abstract

“…[12] This concept has since then widely been appreciated in the biofabrication community. [13][14][15][16][17] Strategies to extend this biofabrication window and allow for printing with good shape fidelity under cytocompatible conditions with as little material content as possible have been an important focus of recent research in the field.…”

Section: Introductionmentioning

confidence: 99%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

View full text Add to dashboard Cite

In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

show abstract

“…A great deal of effort have been made to modulate the rheological properties of hydrogel-based bioinks along these lines. [5] Addition of nano/microparticles is one of the most widely adopted methods to improve rheological properties of a polymer-based hydrogel. [6,7] In particular, nanofibrils or NPs retaining intrinsic shear-thinning properties such as nanocellulose and 2D silicates have been utilized to prepare bioinks in combination with various polymers including gelatin, [8] poly(ethylene glycol)(PEG), [9] carrageenan, [10] hyaluronic acid (HA), [11] alginate, [12] and fibrin.…”

Section: Introductionmentioning

confidence: 99%

Nanocomposite bioink exploits dynamic covalent bonds between nanoparticles and polysaccharides for precision bioprinting

Lee

Bae²,

Levinson³

et al. 2019

Preprint

View full text Add to dashboard Cite

The field of bioprinting has made significant recent progress towards engineering tissues with increasing complexity and functionality. It remains challenging, however, to develop bioinks with optimal biocompatibility and good printing fidelity. Here, we demonstrate enhanced printability of a polymer-based bioink based on dynamic covalent linkages between nanoparticles (NPs) and polymers, which retains good biocompatibility. Amine-presenting silica NPs (ca. 45 nm) were added to a polymeric ink containing oxidized alginate (OxA). The formation of reversible imine bonds between amines on the NPs and aldehydes of OxA lead to significantly improved rheological properties and high printing fidelity. In particular, the yield stress increased with increasing amounts of NPs (14.5 Pa without NPs, 79 Pa with 2 wt% NPs). In addition, the presence of dynamic covalent linkages in the gel provided improved mechanical stability over 7 days compared to ionically crosslinked gels. The nanocomposite ink retained high printability and mechanical strength, resulting in generation of centimetre-scale porous constructs and an ear structure with overhangs and high structural fidelity. Furthermore, the nanocomposite ink supported both in vitro and in vivo maturation of bioprinted gels containing chondrocytes. This approach based on simple oxidation can be applied to any polysaccharide, thus the widely applicability of the method is expected to advance the field towards the goal of precision bioprinting.

show abstract

‘Printability' of Candidate Biomaterials for Extrusion Based 3D Printing: State‐of‐the‐Art

Cited by 347 publications

References 114 publications

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

From Shape to Function: The Next Step in Bioprinting

Nanocomposite bioink exploits dynamic covalent bonds between nanoparticles and polysaccharides for precision bioprinting

Contact Info

Product

Resources

About