3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Wu, Yun; Lin, Ziliang; Wenger, Andrew; Tam, Kam Chiu; Tang, Xiaowu Shirley

doi:10.1016/j.bprint.2017.12.001

Cited by 172 publications

(121 citation statements)

References 31 publications

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“…Apart from an increase in the tensile and compressive strength of the hydrogel, self-healing efficiency, and biocompatibility of the hydrogel improved by 90 and 100%, respectively. Three-dimensional bioprinted constructs composed of either alginate/cellulose nanocrystals or alginate/cellulose nanocrystals containing fibroblast and hepatoma cells, namely bioinks, were introduced by Wu et al as liver-mimetic platforms (Wu et al, 2018). The bioprinted constructs were crosslinked by CaCl 2 .…”

Section: Nano-scaled Cellulose: a Robust Nanomaterialsmentioning

confidence: 99%

Insight Into the Current Directions in Functionalized Nanocomposite Hydrogels

et al. 2020

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Since the introduction of tissue engineering as an encouraging method for the repair and regeneration of injured tissue, there have been many attempts by researchers to construct bio-mimetic scaffolds which mimic the native extracellular matrix, with the aim of promoting cell growth, cell proliferation, and restoration of the tissue's native functionality. Among the different materials and methods of scaffold fabrication, one particularly promising class of materials, hydrogels, has been extensively studied, with the inclusion of nano-scaled materials into hydrogels leading to the creation of an exciting new generation of nanocomposites, known as nanocomposite hydrogels. To closely mimic the native tissue behavior, scientists have recently focused on the functionalization of incorporated nanomaterials via chiral biomolecules, with reported results showing great potential. The current article aims to introduce a perspective of nano-scaled cellulose as a promising nanomaterial which can be multi-functionalized for the fabrication of nanocomposite hydrogels with applications in tissue engineering and drug delivery systems. This article also briefly reviews the recently reported literature on nanocomposite hydrogels incorporated with chiral functionalized nanomaterials. Such knowledge paves the path for the development of tailored hydrogels toward practical applications.

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Section: Nano-scaled Cellulose: a Robust Nanomaterialsmentioning

confidence: 99%

Insight Into the Current Directions in Functionalized Nanocomposite Hydrogels

et al. 2020

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“…Cellulose can be found as nano-crystals, nanofibers, or in bacterial form, where they can have high surface area. Nano-cellulose crystals and fibers have been widely mixed with alginate to form bioinks which have excellent shear-thinning properties allowing improvement in printability as well as fast cross-linking to achieve shape fidelity [244,[266][267][268][269][270][271][272]. For light-assisted printing, cellulose has been mainly used as a filler and reinforcement material to stiffen and strengthen hydrogels and soft polymers.…”

Section: Process and Materialsmentioning

confidence: 99%

“…The encapsulation of chondrocytes for cartilage tissue engineering [244,269,271], human derived induced pluripotent stem cells [266], human bone marrow derived mesenchymal stem cells [272], pancreatic cancer cells [268], and fibroblast and hepatoma cells [270], all showed increased bioactivity such as cell expression, proliferation, and viability. However, a more detailed study of printing process parameters show that optimum pressure, shear stress, and nozzle size can greatly affect the bioactivity of the encapsulated cells, i.e., for nozzle diameters below 400 µm, cell morphology and proliferation suffered, while for nozzle diameters below 200 µm cell viability also was affected [267].…”

Section: Biocompatibility Biodegradability and Bioactivitymentioning

confidence: 99%

“…Inclusion of NiCu [237], e-polylysine [238], carrageenan [239], gelatin [240][241][242], cellulose [243,244], PVA [252], TiO 2 [253], β-TCP [253]; Feature size 150 µm, E: 280 kPa [240] Myoblasts [242]; endothelial cells [259]; E.coli [260], growth factor [250], human adipose stem cells [248]; human induced pluripotent stem cells [261]; chondrocytes [243,244,251]; Schwann cells [265] Cellulose (4.3.4) Mixed with alginate [244,[266][267][268][269][270][271][272]; As reinforcement material, E: 2.5-22.5 kPa [281] Feature size 500 µm [280]; Tensile E: 0.67-0.63 GPa [282] Chondrocytes for cartilage tissue engineering [244,269,271]; human induced pluripotent stem cells, bone-marrow hMSCs [272]; pancreatic cancer cells [268]; fibroblast and hepatoma cells [270]; NIH 3T3 cells [274] Collagen (4.4.1)…”

Section: ) Applicationsmentioning

confidence: 99%

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Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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“…Mitogenic hydrogel system was developed by using cellulose nanofibres along with alginate sulphate. [15][16][17] Thus, this review initially emphasizes on emerging field of 3D bioprinting along with its future perspective, advancements in formulation and development of cellulosic bioink as a novel, imminent field of biomaterials research.…”

Section: Introductionmentioning

confidence: 99%

Biomaterials in 3D Printing: A Special Emphasis on Nanocellulose

Badhe¹,

Godse²,

Ahinkar³

2020

IJPER

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Aim and Background: The main objective of this review is to highlight nanocellulosic materials in 3D bioprinting. Three-dimensional (3D) bioprinting is on the verge of fabricating the artificial organ and living tissues. For the target construction the process of this 3D bioprinting involves layer-by-layer deposition of suitable biomaterials using predesigned data made by using Computer Aided Design (CAD) as an outline. However, only a handful of biomaterials are able to fulfil the considerable requirements for suitable bioink formulation, which is a critical component of efficient 3D bioprinting. Conclusion: Cellulose, a naturally occurring polysaccharide, is clearly the most commonly employed material in current bioinks. Here, in this review we discuss the advantages, reasons, applications, disadvantages of the use of cellulosic bioink in 3D bioprinting by summarizing the most recent studies that used cellulose for printing vascular tissue, bone and cartilage. In addition, other breakthroughs in the use of cellulose in bioprinting are discussed, including strategies to improve its structural and degradation characteristics. In this review, we organize the available literature in order to inspire and accelerate novel cellulose-based bioink formulations with enhanced properties for future applications in basic research, drug screening and regenerative medicine.

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3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Cited by 172 publications

References 31 publications

Insight Into the Current Directions in Functionalized Nanocomposite Hydrogels

Insight Into the Current Directions in Functionalized Nanocomposite Hydrogels

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Biomaterials in 3D Printing: A Special Emphasis on Nanocellulose

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