Principles and Methods Used in Yeast Classification, and an Overview of Currently Accepted Yeast Genera

Boekhout, Teun; Kurtzman, Cletus P.

doi:10.1007/978-3-642-79856-6_1

Cited by 39 publications

(41 citation statements)

References 212 publications

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“…Ascospores formation by the yeast isolate was detected for indication of the ascomycetous yeast. The yeast isolate can produce pseudomycelium and showed in a filamentous form under microscope and can utilize glucose, fructose, sucrose, maltose and trehalose but failed to grow on lactose and xylose, rhamnose, raffinose and arabinose, which is characteristic of Saccharomyces cerevisiae [46]. 5.8 s rDNA sequencing revealed the identity of the isolate to be Saccharomyces cerevisiae (Accession no- HM134859.1).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

136

106

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BackgroundProbiotic yeast has become a field of interest to scientists in recent years.MethodsConventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties.ResultsThis study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme. It can produce organic acid and showed resistance against tetracycline, ampicillin, gentamycin, penicillin, polymixin B and nalidixic acid. It can assimilate cholesterol, can produce killer toxin, vitamin B12, glutathione, siderophore and strong biofilm. It showed moderate auto-aggregation ability and cell surface hydrophobicity. The isolate can produce enzymes such as amylase, protease, lipase, cellulose, but unable to produce galactosidase. The isolate can’t produce gelatinase and DNase. The isolate showed moderate anti-microbial activity against bacteria and fungi and cell lysate showed better antimicrobial activity than whole cell and culture supernatant. Again, the isolate showed better anti-bacterial activity against gram negative bacteria than gram positive. The isolate showed strong antioxidant activity, reducing power, nitric oxide and hydroxyl radical scavenging activity, significant brine shrimp cytotoxicity and acute toxicity and metal ion chelating activity. The isolate did not induce any detectable change in general health of mice upon oral toxicity testing and found to be safe in mouse model. The isolate improve lymphocyte proliferation and cytokine production in treated mice.ConclusionsSuch isolate could be potential as probiotic to be used therapeutically.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

136

106

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“…Based on their colony characteristics (white and creamy texture), ovoid microscopic shape, and the presence of budding pattern (multipolar), all isolates that were found belongs to saccharomyces type unicellular ascomycete according to standard methods [13, 14]. The isolates were tested for fermentation of carbohydrates; isolate Pa was able to utilize 6 sugars out of the seven tested sugars, and Or isolate was able to utilize 5 sugars, indicating that they were diverse in sugar utilization.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production

Nasir

Rahman

Hossain

et al. 2017

EuJMI

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In view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. The main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. Two yeast isolates from pineapple (Pa) and orange (Or) have been isolated, characterized on the basis of morphological and physic-chemical characters and optimized on ethanol producing capability using sugarcane molasses as substrate. Ethanol production percentage was estimated by Conway method. Isolates were thermotolerant, pH tolerant, ethanol tolerant as well as osmotolerant. They were resistant to Chloramphenicol (30 μg/disc) and Nalidixic acid (30 μg/disc). The isolates showed no killer toxin activity against E. coli. The highest production capacity of the yeasts was found to be 7.39% and 5.02% for Pa and Or, respectively, at pH 5.0, 30 °C temperature in media with an initial reducing sugar concentration of 6.5% for Pa and 5.5% for Or (shaking). Addition of metal ions increased the rate of ethanol production highest to 10.61% by KH2PO4. This study revealed that indigenous yeast isolates could be used to benefit the fuel demand and industrial alcohol industries.

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“…Yeasts were identified based on their large subunit ribosomal DNA (LSU) sequence (partial 28S rDNA, D1/D2 domains), amplified and sequenced with primers NL1 and NL4 (Boekhout & Kurtzman 1996); when species groups were involved, further characterization has not been performed. BigDye terminator cycle sequencing kits were used in sequence reactions (Applied Biosystems, Foster City, CA, U.S.A.).…”

Section: Molecular Characterizationmentioning

confidence: 99%

Dishwashers – A man-made ecological niche accommodating human opportunistic fungal pathogens

et al. 2011

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Principles and Methods Used in Yeast Classification, and an Overview of Currently Accepted Yeast Genera

Cited by 39 publications

References 212 publications

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production

Dishwashers – A man-made ecological niche accommodating human opportunistic fungal pathogens

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