Principle of a Counter-streaming Centrifuge for the Separation of Particles of Different Sizes

Lindahl, Per Eric

doi:10.1038/161648a0

Cited by 128 publications

(27 citation statements)

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“…Both in volumetric and surface analysis, insulin-containing B cells were found to be two-to three-fold larger than the other islet cell types (Fig.2, [ ty than islet non-B cells. Centrifugal elutriation, which separates cells according to their sedimentation velocity [18][19][20], distributed the islet cells over a first single cell fraction enriched in islet non-B cells, a second single cell fraction composed of more than 90 percent B cells and a third fraction of mostly structurally coupled cells [16]. The knowledge that islet B cells were separable on the basis of their larger size [16,17] led to attempts to purify islet cells by flow cytometry, with the cellular light scatter activity as separation parameter.…”

Section: The Purification Of Islet Cellsmentioning

confidence: 99%

The biosociology of pancreatic B cells

1987

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Section: The Purification Of Islet Cellsmentioning

confidence: 99%

The biosociology of pancreatic B cells

1987

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“…One such technique is counterflow centrifugal elutriation, which uses centrifugal force balanced by a centripetal flow of fluid in a specially designed centrifuge rotor to separate the cells by their sedimentation velocity, which is dependent upon cell size and shape. 17,18,19 Although this method successfully isolates different sizes of cells, with the smallest cells assumed to be in G1 phase, it exposes them to harsh conditions, such as a 683 x g rotor speed at 20°C for 4-5 hours. 19 More recently, cells have been separated by size in microfluidic devices using dielectrophoresis, acoustophoresis, hydrophoresis, hydrodynamic filtration, or inertial forces.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic “baby machine” for cell synchronization

et al. 2012

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Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the "baby machine" was developed previously. In the technique, suspension cells are attached to a membrane, and as the cells divide, the newborn cells are eluted to produce a synchronous population of cells in the G1 phase of the cell cycle.However, the existing "baby machine" is only suitable for cells which can be chemically attached to a surface. Here, we present a microfluidic "baby machine" in which cells are held onto a surface by pressure differences rather than chemical attachment. As a result, our method can in principle be used to synchronize a variety cell types, including cells which may have weak or unknown surface attachment chemistries. We validate our microfluidic "baby machine" by using it to produce a synchronous population of newborn L1210 mouse lymphocytic leukemia cells in G1 phase.

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“…Briefly, the law states that the sedimentation velocity (SV) of a nondeformable particle moving through a stationary liquid under the influence of a centrifugal field is proportional to the square of the effective diameter of the particle (d) multiplied by the difference between the density of the particle and the density of the liquid (ρ p -ρ m ) divided by the product of the liquid viscosity (η) and the "shape constant" of the particle (k, its deviation from sphericity) all times the square of the angular velocity of the rotating system at radius r (w 2 r). As was recognized first by Lindahl (1948), the same equation applies to a stationary particle in a moving liquid flow. The analysis of Sanderson and Bird led to the derivation of Equation 3, an expression which states that "there is a radius r (defined by evaluation of Equation 1) at which a particle is immobilized in a liquid flowing at velocity (V) in a centrifugal field" (the parameters of Equation 3 are those defined above where (ρ p -ρ m ) has been replaced by (ρ')).…”

Section: Theory Of the Centrifugal Bioreaction Processmentioning

confidence: 71%

Centrifugal Bioreactors and Their Application in Remediation

Herman¹,

Lin²,

Petrecca³

et al. 2001

Remediation Journal

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Principle of a Counter-streaming Centrifuge for the Separation of Particles of Different Sizes

Cited by 128 publications

References 0 publications

The biosociology of pancreatic B cells

The biosociology of pancreatic B cells

A microfluidic “baby machine” for cell synchronization

Centrifugal Bioreactors and Their Application in Remediation

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