Primordium of an artificial Bruch’s membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers

Warnke, Patrick H.; Alamein, Mohammad A.; Skabo, Stuart; Stephens, Sebastien Robert; Bourke, Robert D.; Heiner, Peter; Liu, Qin

doi:10.1016/j.actbio.2013.07.029

Cited by 98 publications

(103 citation statements)

References 51 publications

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“…The mechanical properties of ABC+UBM are comparable to the ones reported in a recent study, where nanofibrous membranes of poly(lactic-co-glycolic acid) (PLGA) and collagen type-I (the main component of UBM) were evaluated as potential BM substitutes [3]. The values obtained for the PLGA nanofibrous membrane were 1.5 ± 0.4 MPa, 28.8 ± 4.9 % and 131.9 ± 13.3 MPa for maximum tensile strength, tensile strain and elastic modulus, respectively.…”

Section: Substrate Characterizationsupporting

confidence: 79%

“…BC substrates were analyzed for the presence of endotoxin using the LAL kit according to the supplier instructions. Each sterile ABC substrates of approximately 0.88 mm 3 , with and without UBM, were immersed in 40 mL pyrogen-free water. The reaction was stopped with 25 % acetic acid and the absorbance measured at 405 nm.…”

Section: Swelling/ Volume = Mass Wet T −Mass Drymentioning

confidence: 99%

“…To evaluate the morphology of the RPE cell monolayers grown on the developed substrates, several cell parameters may be assessed, namely the expression of RPE proteins such as ZO-1 and RPE65, the observation of a polygonal shaped cells with apical microvilli in a monolayer configuration and the development of TER [1,3,4]. The RPE characteristic phenotype can be ascertained by the expression of RPE specific proteins.…”

Section: Rpe Cell Morphologymentioning

confidence: 99%

“…6. C, D), which was expressed in small amounts when compared to ARPE-19 cells and primary cells [3,38].…”

Section: Rpe Cell Morphologymentioning

confidence: 99%

“…This monolayer is maintained and stabilized by the cytoskeleton of individual cells and their interactions at the basolateral junctional complexes [2][3][4]. These junctional complexes or tight junctions are composed of zonular occludens-1 (ZO-1), occludin, claudins 1, 2, 5, 12 and AL [1].…”

Section: Introductionmentioning

confidence: 99%

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Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves

Rodrigues

Padrão

et al. 2016

Colloids and Surfaces B: Biointerfaces

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This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored. Therefore, the ABC substrates coated with UBM showed a great potential to be applied as cell carriers in RPE transplantation.

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Section: Substrate Characterizationsupporting

confidence: 79%

Section: Swelling/ Volume = Mass Wet T −Mass Drymentioning

confidence: 99%