PrimaryCampylobacter jejuniinfection in different mice strains

Vučković, Darinka; Abram, Maja; Dorić, Miljenko

doi:10.1006/mpat.1997.0194

Cited by 34 publications

(29 citation statements)

References 11 publications

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“…Different animal models have been used for Campylobacter research, including mice (3,6,29,31), rabbits (7), ferrets (4,11), hamsters (14), chicks (26), piglets (1), dogs (21), and Old World monkeys (i.e., Macaca nemestrina, Macaca mulatta, and Macaca fascicularis) (10,24,25). Animal models in which Campylobacter spp.…”

mentioning

confidence: 99%

New World Monkey Aotus nancymae as a Model for Campylobacter jejuni Infection and Immunity

et al. 2006

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confidence: 99%

New World Monkey Aotus nancymae as a Model for Campylobacter jejuni Infection and Immunity

et al. 2006

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“…We demonstrated the usefulness of the gfp reporter system in an in vivo system by intraperitoneally injecting mice with C. jejuni that had been transformed with a gfp-containing vector. While mice do not represent a suitable model for Campylobacter-mediated enteritis, investigators have used intraperitoneal injection of mice to examine the interaction of C. jejuni with various components of the immune system (17,18,29). In conjunction with flow cytometry analysis, we demonstrate the application of the new vector containing gfp to show the cellular subset associated with C. jejuni in BALB/c mice.…”

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confidence: 99%

In Vivo Tracking ofCampylobacter jejuniby Using a Novel Recombinant Expressing Green Fluorescent Protein

Mixter

Klena

Flom

et al. 2003

Appl Environ Microbiol

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Campylobacter jejuni is a leading cause of food-borne disease in developed countries. The goal of this study was to develop a plasmid-based reporter system with green fluorescent protein (GFP) to facilitate the study of C. jejuni in a variety of niches. C. jejuni transformants harboring the pMEK91 GFP gene (gfp)-containing vector were readily detectable by both fluorescence microscopy and flow cytometry. Given the ease of detecting these organisms, additional experiments were performed in which BALB/c mice were injected intraperitoneally with C. jejuni harboring the gfp-containing vector. Four hours after injection of the mice, flow cytometry analyses determined that C. jejuni synthesizing GFP were predominantly associated with granulocytes. More specifically, the proportion of CD11b ؉ Gr-1 ؉ lavage neutrophils with green fluorescence ranged from 99.7 to 100%, while the proportion of CD11b ؉ Gr-1 ؊ lavage macrophages ranged from 77.0 to 80.0%. In contrast, few CD11b ؊ CD45R ؉ B lymphocytes from the lavage of the C. jejuni-injected mice were associated with greenfluorescent C. jejuni (proportions ranged from 0.75 to 0.77%). Cell-free C. jejuni was recovered from tissue homogenates after intraperitoneal injection. Macrorestriction profiling with pulsed-field gel electrophoresis identified a genotypic variant of the C. jejuni F38011 wild-type isolate. In vivo this variant displayed a phenotype identical to that of the wild-type isolate. In summary, we demonstrate that C. jejuni associates with marker-defined cellular subsets in vivo with a novel gfp reporter system and that C. jejuni genotypic variants can be isolated from both in vitro and in vivo systems.

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“…infection. [25][26][27] We thus followed the systemic spread and infection for the internal organs (i.e., liver, spleen) after intravenous infection of BALB/c mice with this human clinical isolate of C. coli. We followed the course of infection and cytokine production.…”

Section: Discussionmentioning

confidence: 99%

“…The C. coli 26536 CFU in these organs were determined as previously described. 25 At least 6 mice per time point were infected. For organ burden determination, at least 3 mice were sacrificed per time point, and at least another 3 for cytokine analyses.…”

Section: Bacterial Isolatementioning

confidence: 99%

“…In previous studies, we showed that C. jejuni can successfully invade epithelial cells in vitro 8,40 and produce systemic infection in vivo. 25 Our earlier data obtained in vitro showed that C. jejuni can survive both in phagocytes (for 72 h) and in intestinal epithelial cells (for 96 h). This is in agreement with the conclusions of Day et al (2000), 41 who considered C. jejuni to be a facultative intracellular pathogen, and not a typical extracellular bacterium.…”

Section: Introductionmentioning

confidence: 95%

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Virulence genes and cytokine profile in systemic murineCampylobacter coliinfection

et al. 2015

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PrimaryCampylobacter jejuniinfection in different mice strains

Cited by 34 publications

References 11 publications

New World Monkey Aotus nancymae as a Model for Campylobacter jejuni Infection and Immunity

New World Monkey Aotus nancymae as a Model for Campylobacter jejuni Infection and Immunity

In Vivo Tracking ofCampylobacter jejuniby Using a Novel Recombinant Expressing Green Fluorescent Protein

Virulence genes and cytokine profile in systemic murineCampylobacter coliinfection

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