Virulence genes and cytokine profile in systemic murine<i>Campylobacter coli</i>infection

Klančnik, Anja; Pogačar, Maja Šikić; Raspor, Peter; Abram, Maja; Možina, Sonja Smole; Vučković, Darinka

doi:10.1080/21505594.2015.1042642

Cited by 9 publications

(5 citation statements)

References 56 publications

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“…The observed reduced expression levels of IL-6 and IL-23a in similar groups could be due to the increased expression of IL-10 in the liver. A significant increase in IL-10 production in the liver has been demonstrated in Campylobacter -infected BALB/c mice, while IL-6 production was not significant ( 43 ). In general, IL-10 was downregulated or not expressed in ASC-treated groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sublethally Injured Campylobacter jejuni in Mice

et al. 2022

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Campylobacter is the most common cause of bacterial gastrointestinal disease, and consumption of contaminated poultry is frequently identified as the source of bacteria. The survivability and virulence potential of sublethally injured Campylobacter following exposure to chemicals which are commonly used to eliminate Campylobacter during the poultry meat processing are of concern to the food industry, government health officials, and consumers.

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Section: Discussionmentioning

confidence: 99%

Effects of Sublethally Injured Campylobacter jejuni in Mice

et al. 2022

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“…Research on C. coli has been widely neglected to the present date, although the pathogen is the second most common causative agent of human Campylobacter infections after C. jejuni [33]. Despite the fact that several studies point towards genetic differences between C. coli and C. jejuni, knowledge regarding the phenotypic differences is virtually lacking [34][35][36]. Very recently, our group demonstrated that, in contrast to C. jejuni, a C. coli patient isolate could override the colonization resistance of conventionally colonized wildtype mice, and remained in the large intestine up to three weeks post-infection [37].…”

Section: Discussionmentioning

confidence: 99%

Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota

et al. 2020

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Zoonotic Campylobacter, including C. jejuni and C. coli, are among the most prevalent agents of food-borne enteritis worldwide. The immunopathological sequelae of campylobacteriosis are caused by Toll-like Receptor-4 (TLR4)-dependent host immune responses, induced by bacterial lipooligosaccharide (LOS). In order to investigate C. coli-host interactions, including the roles of the human gut microbiota and TLR4, upon infection, we applied a clinical acute campylobacteriosis model, and subjected secondary abiotic, TLR4-deficient IL10-/- mice and IL10-/- controls to fecal microbiota transplantation derived from human donors by gavage, before peroral C. coli challenge. Until day 21 post-infection, C. coli could stably colonize the gastrointestinal tract of human microbiota-associated (hma) mice of either genotype. TLR4-deficient IL10-/- mice, however, displayed less severe clinical signs of infection, that were accompanied by less distinct apoptotic epithelial cell and innate as well as adaptive immune cell responses in the colon, as compared to IL10-/- counterparts. Furthermore, C. coli infected IL10-/-, as opposed to TLR4-deficient IL10-/-, mice displayed increased pro-inflammatory cytokine concentrations in intestinal and, strikingly, systemic compartments. We conclude that pathogenic LOS might play an important role in inducing TLR4-dependent host immune responses upon C. coli infection, which needs to be further addressed in more detail.

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“…This is in accordance with Klancnik et al . [ 15 ] who found beside the high level of anti-inflammatory cytokines, a pronounced increase in IL-10 in the plasma between day 1 and 8 post infection in mice infected with C . coli .…”

Section: Discussionmentioning

confidence: 99%

“…jejuni [ 8 , 9 , 14 ] and C . coli was also reported [ 15 ]. Beside its inflammatory action, IL-6 plays a critical role in governing the transition from innate to acquired immunity [ 13 ].…”

Section: Introductionmentioning

confidence: 86%

Temporal induction of pro-inflammatory and regulatory cytokines in human peripheral blood mononuclear cells by Campylobacter jejuni and Campylobacter coli

2017

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Campylobacter jejuni along with C. coli are major cause of human gastroenteritis worldwide. So far, the human immune response against Campylobacter is not entirely clear. We hypothesize that it is coordinated by an interaction between pro-inflammatory and regulatory cytokines which is influenced by bacterial and host-individual differences. Accordingly, we used peripheral blood mononuclear cells (PBMC) from healthy donors to study the primary systemic immune response to C. jejuni and C. coli. PBMC were stimulated by different strains of C. jejuni and C. coli for three time points (5, 10, 24 hours). The production of the pro-inflammatory (IL-6, IL-8, IFN-γ) and the regulatory (IL-10) cytokines were measured by ELISA. All strains induced higher levels of IL-8 and IL-6 than IFN-γ and IL-10. In contrast to IL-8 and IL-6, IL-10 showed a steeper increase over time. While IFN-γ did not show any further increase between 10 and 24 hours. Interestingly, there was a significant correlation between IL-8 and IL-10 which peaked at 24 hours. Despite the variability of the used bacterial strains, their effect on cytokine production was less pronounced than the inter-person differences. The strongest significant effect of the strain was on the level of IL-10. IL-10 and IL-6 were significantly influenced by strain-person interaction. In conclusion, the systemic immune response to C. coli and C. jejuni is characterized by an early pro-inflammatory reaction with later initiation of regulatory immune response which is influenced mainly by the host, explaining the individual variations in disease severity. Additional work is needed to determine the cellular sources of the produced cytokines as well as the campylobacter molecules that might contribute to this stimulation.

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Virulence genes and cytokine profile in systemic murineCampylobacter coliinfection

Cited by 9 publications

References 56 publications

Effects of Sublethally Injured Campylobacter jejuni in Mice

Effects of Sublethally Injured Campylobacter jejuni in Mice

Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota

Temporal induction of pro-inflammatory and regulatory cytokines in human peripheral blood mononuclear cells by Campylobacter jejuni and Campylobacter coli

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