Primary structures and gene organizations of two types of Wap65 from the pufferfish Takifugu rubripes

Hirayama, Makoto; Nakaniwa, Makiko; Ikeda, Daisuke; Hirazawa, Noritaka; Otaka, T.; Mitsuboshi, Toru; Shirasu, Kunio; Watabe, Shugo

doi:10.1023/b:fish.0000045723.52428.5e

Cited by 30 publications

(39 citation statements)

References 40 publications

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“…Further analyses on other fish species (Oncorhynchus mykiss, Ictalurus punctatus, Danio rerio, Oryzias latipes and Takifugu rubripes) have shown that in contrast to the single isoform found in mammals, wap65 is present in duplicate in fish, presumably as a result of the teleost whole genome duplication event (Amores et al, 1998). These fish isoforms display different affinities for haem, tissue distributions and development patterns (Hirayama et al, 2003;2004;Nakaniwa et al, 2005). In contrast to the eurythermal examples quoted earlier, some animals exist within a very narrow environmental temperature envelope.…”

Section: Introductionmentioning

confidence: 81%

“…Wap65-2 has a much more restricted tissue distribution (mainly liver), compared to wap65-1, which is present in multiple tissues in O. latipes and Takifugu (Hirayama et al, 2003;2004). In spite of the presence of the two conserved histidine residues proposed to be essential for haem binding, other fish orthologues of Wap65-2, which also contain these residues, do not show an affinity for haem (Hirayama et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…This gene set has also been shown to be induced in response to immunological stimulus using LPS and hypoxia in C. auratus (Kikuchi et al, 1997;Gracey et al, 2001). However, there is no elevation in the expression of either of the two wap65 isoforms to induction by LPS in O. latipes (Hirayama et al, 2004) or to environmental temperature increases in O. latipes and T. rubripes (Hirayama et al, 2003). Indeed, so far, the correlation of increased expression levels of wap65 with increased environmental temperature have only been identified in the Cypriniformes, specifically the wap65-1 isoform.…”

Section: Discussionmentioning

confidence: 99%

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Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus)

Clark

Burns

2008

DNA Sequence

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Physiological adaptation to increased environmental temperatures has been studied experimentally in a number of fish species, with the up-regulation of several genes identified as being associated with the process, such as the warm-acclimated protein (wap-65). This article describes the cloning and characterisation of the wap65-2 gene from the Antarctic plunderfish (Harpagifer antarcticus). The transcriptional expression of this gene in response to elevated seawater temperatures over a time course series is presented. Initially there is strong down regulation of this gene to a maximum of 40 fold within 4 hours, followed by recovery to almost control levels within 48 hours, indicating that this gene does not play a role in the potential temperature adaptation of H. antarcticus. WAP65

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Section: Introductionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus)

Clark

Burns

2008

DNA Sequence

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“…Wap65 was first detected as a glycoprotein in the plasma of goldfish (Kikuchi et al 1993) and has since been identified in medaka (Oryzias latipes), channel catfish (Ictalurus punctatus), swordtail fish (Xiphophorus hellerii), and fugu (Takifigu rubripes) (Kikuchi et al 1995;Kinoshita et al 2001;Hirayama et al 2003Hirayama et al , 2004Nakaniwa et al 2005;Aliza et al 2008). Teleost Wap65 shares significant sequence identity with mammalian hemopexin and has been suggested to play roles in iron homeostasis, antioxidant activity, bacteriostatic defense, nerve regeneration, transfer of heme, and the expression of genes known to promote cell survival (Delanghe and Langlois 2001).…”

Section: Introductionmentioning

confidence: 99%

Environmental stress-related gene expression and blood physiological responses in olive flounder (Paralichthys olivaceus) exposed to osmotic and thermal stress

Choi

2010

Animal Cells and Systems

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We isolated warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of olive flounder and investigated the mRNA expression of Wap65 and HSP70 in olive flounder exposed to osmotic (17.5, 8.75, and 4 psu) and thermal stress (25 and 308C). The mRNA expression of Wap65 and HSP70 was increased by thermal stress. The mRNA expression of HSP70 was also increased by osmotic stress, whereas no significant change in Wap65 expression was detected. These results indicate that Wap65 mRNA expression occurs specifically in response to increases in water temperature, but not in response to osmotic stress. Plasma cortisol levels were also increased by osmotic and thermal stress. We also utilized the stress hormone cortisol to examine whether Wap65 expression is thermal-stress-specific. Cortisol treatment increased HSP70 mRNA expression in vitro, but had no significant effect on Wap65 mRNA expression. Thus, thermal stress, but not osmotic stress, induces Wap65 expression.

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“…因其与暖温适应相关且相对分子质量为 6.5×10 4 而得名。该类蛋白存在 Wap65-1 和 Wap65-2 两种异构体 (Hirayama et al, 2003)。研究表明, Wap65-2 具有多种生物学功能, 它与鱼类暖温适应 (Hirayama et al, 2003)、重金属胁迫 (Aliza et al, 2008)和生长发育 (Hirayama et al, 2004) 等紧密相关。近年来 , Wap65-2 在细菌感染性免疫应激中的作用日益引起人们的关注 (Cho et al, 2012;Li et al, 2011;Peatman et al, 2008;Sha et al, 2008;Shi et al, 2010)。目前, 多种鱼类 Wap65-2 基因序列已被克隆, 包括东方红鳍鲀(Takifugu rubripes) (Hirayama et al, 2003)、青鳉 (Oryzias latipes) (Hirayama et al, 2004) 、蓝鲇 (Ictalurus furcatus) (Peatman et al, 2008)、斑点叉尾鮰 (I. punctatus) (Sha et al, 2008) 、剑尾鱼 (Xiphophorus hellerii) (Aliza et al, 2008)、真裸南极鱼(Harpagifer antarcticus) (Clak & Burns, 2008)、香鱼(Plecoglossus altivelis) (Shi et al, 2010)、欧洲海鲈 (Dicentrarchus labrax) (Sarropoulou et al, 2010)和大鳞泥鳅(Misgurnus mizolepis) (Cho et al, 2012 (Li et al, 2009)…”

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Cloning, physical and chemical property analysis of the Japanese sea bass Wap65-2 gene and its expression following <I>Vibrio harveyi</I> infection

Shi

Chen²,

Gao³

et al. 2013

Zoological Research

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The warm temperature acclimation related 65 kDa protein-2 (Wap65-2), a teleost plasma glycoprotein, plays an important role in immune regulation against bacterial infection. Here, for the first time we determined the full length cDNA sequence of the Japanese sea bass Wap65-2 gene (1 601 bp in length excluding the 3'-polyA tail). The sequence contains an open reading frame that encodes a protein of 436 amino acids with a molecular weight of 4.87×10 4 . The predicted protein had a signal peptide in the N-terminal domain containing 19 residues. Sequence comparison and phylogenetic tree analysis showed that the Japanese sea bass Wap65-2 has a relatively high similarity to the Dicentrarchus labrax Wap65-2. In the healthy Japanese sea bass, Wap65-2 mRNA was expressed mainly in the liver and weakly in the heart and muscle. qRT-PCR results revealed that liver Wap65-2 transcripts were significantly increased after a Vibrio harveyi infection, and peaked 24 hour post injection (6.89 fold increase). The Japanese sea bass Wap65-2 protein was expressed in Escherichia coli and subsequently used for antiserum preparation. Western blot analysis showed that Wap65-2 was significantly increased in V. harveyi infected Japanese sea bass and reached a maximum of 5.33-fold increase at 36 h. In conclusion, the alteration of Japanese sea bass Wap65-2 expression was tightly associated with the progression of the V. harveyi bacterial infection.Key words: Wap65-2; Lateolabrax japonicus; Vibrio Harveyi; qRT-PCR; Western blot Wap65s (warm temperature acclimation related 65 kDa protein)是鱼类中发现的一类血浆糖蛋白,

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Primary structures and gene organizations of two types of Wap65 from the pufferfish Takifugu rubripes

Cited by 30 publications

References 40 publications

Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus)

Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus)

Environmental stress-related gene expression and blood physiological responses in olive flounder (Paralichthys olivaceus) exposed to osmotic and thermal stress

Cloning, physical and chemical property analysis of the Japanese sea bass Wap65-2 gene and its expression following <I>Vibrio harveyi</I> infection

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