Primary Structure of a Proline-Rich Zein and Its cDNA

Wang, Shuzhen; Esen, Asım

doi:10.1104/pp.81.1.70

Cited by 45 publications

(33 citation statements)

References 19 publications

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“…However there was no pronounced effect of ionic strength on the particle size of zein nanoparticles. Zein has relatively a lesser proportion of charged amino acids compared other proteins such as albumin (9). The results suggest that the zein nanoparticles are sterically stabilized (25) by the surfactants and therefore the electrostatic interactions may not play a predominant role.…”

Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

“…It consists of three fractions that vary in molecular weight (MW) and solubility (9). These include α-zein (MW, 19-24 kDa; 75-80% of total protein), β-zein (17-18 kDa, 10-15%), and γ-zein (27 kDa, 5-10%) (9). The large proportion (>50%) of non-polar amino acids (leucine, proline, alanine, and phenylalanine) in zein makes it water insoluble (10).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Formulation Factors on the Preparation of Zein Nanoparticles

2012

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Abstract. The main objective of the present study was to investigate the influence of various formulation parameters on the preparation of zein nanoparticles. 6,7-dihydroxycoumarin (DHC) was used as a model hydrophobic compound. The influence of pH of the aqueous phase, buffer type, ionic strength, surfactant, and zein concentration on particle size, polydispersity index, and zeta potential of DHC-loaded zein nanoparticles were studied. Smaller nanoparticles were formed when the pH was close to the isoelectric point of zein. DHC-loaded zein nanoparticles prepared using citrate buffer (pH 7.4) was better than phosphate buffer in preventing particle aggregation during lyophilization. The ionic strength did not have a significant influence on the particle size of DHC-loaded zein nanoparticles. A combination of Pluronic F68 and lecithin in 2:1 ratio stabilized the zein nanoparticles. An increase in zein concentration led to increase in particle size of DHC-loaded zein nanoparticles. The use of optimal conditions produced DHCloaded nanoparticles of 256±30 nm and an encapsulation efficiency of 78±7%. Overall, the study demonstrated the optimal conditions to prepare zein nanoparticles for drug encapsulation.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Formulation Factors on the Preparation of Zein Nanoparticles

2012

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“…Several genes have been shown to be expressed in an inducible, tissue-specific or developmentally controlled manner in transgenic plants (8,24,26,35). Promoters have been dissected into various expression signals by using this approach (8,9,24); however, there are many levels of control that remain undefined (3,31,43). For example, position effects have been observed in which the same construct gives considerable variation in expression levels between individual transformants (8,24,35).…”

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confidence: 99%

Allelic variation and differential expression at the 27-kilodalton zein locus in maize.

Das¹,

Messing²

1987

Mol. Cell. Biol.

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Allelic variation between inbred lines at the 27-kilodalton zein gene locus in maize has been used to study gene expression in developing endosperm. The inbred lines W22 and W23 contain two genes for this protein within two tandem repeats; the individual genes are virtually identical, with 99.9% homology in the 5'-flanking regions. Using gene-specific oligonucleotide probes, we have shown that transcripts of the downstream gene are found at a 2.5-fold-higher level than those of the upstream gene. Another inbred line, BSSS53, has one copy of the gene which is a recombinant of the duplicated genes at the 3'-flanking region. This line has been used in reciprocal crosses to demonstrate dosage effects for the overexpression of the downstream gene and to show that the overexpression of mRNA is reflected in a corresponding increase in the protein level. The accumulation of the protein through development does not, however, always correspond to the difference in mRNA levels.

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“…The coding region of this gene contains 671 bp and no introns are found in the coding region. Wang and Esen ( 1986) reported that the y-zein contains 204 amino acid residues. The predicted molecular weight of this protein is about 27-kD.…”

Section: Genetics and Primary Structure Of Zeinsmentioning

confidence: 99%

The 1Dx5 high molecular weight subunit gene from wheat in transgenic maize

Sangtong¹

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Primary Structure of a Proline-Rich Zein and Its cDNA

Cited by 45 publications

References 19 publications

Influence of Formulation Factors on the Preparation of Zein Nanoparticles

Influence of Formulation Factors on the Preparation of Zein Nanoparticles

Allelic variation and differential expression at the 27-kilodalton zein locus in maize.

The 1Dx5 high molecular weight subunit gene from wheat in transgenic maize

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