1989
DOI: 10.1093/nar/17.23.9679
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Primary structure and regulation of vegetative specific genes ofDictyostelium discoideum

Abstract: We have examined the expression and structure of several genes belonging to two classes of vegetative specific genes of the simple eukaryote, Dictyostelium discoideum. In amebae grown on bacteria, deactivation of all vegetative specific genes occurred at the onset of development and very little mRNA exists by 8 to 10 hours. In contrast, when cells were grown in axenic broth, the mRNA levels remained constant until a dramatic drop occurred around 10 to 12 hours. Thus, regulation of both classes of genes during … Show more

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Cited by 47 publications
(26 citation statements)
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References 28 publications
(27 reference statements)
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“…Sequence comparison of EDF-1 by the BLASTX program revealed 66% identity at the amino acid level to MBF1 (multiprotein-bridging factor 1) of the silkworm Bombyx mori, a transcriptional coactivator that may play a role in differentiation (17), and 46% identity to H7, a developmentally regulated gene of Dictyostelium discoideum (18,19). In Fig.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Sequence comparison of EDF-1 by the BLASTX program revealed 66% identity at the amino acid level to MBF1 (multiprotein-bridging factor 1) of the silkworm Bombyx mori, a transcriptional coactivator that may play a role in differentiation (17), and 46% identity to H7, a developmentally regulated gene of Dictyostelium discoideum (18,19). In Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Cells-The possibility that EDF-1 is modulated in endothelial differentiation is suggested by the similarity between EDF-1 and H7, a developmentally regulated gene isolated in D. discoideum (18,19) (Fig. 1C).…”
Section: Edf-1 Expression In Differentiating Endothelialmentioning
confidence: 99%
“…1) consisting of the Dictyostelium V18 promoter, a polylinker, and the terminator of actin 8, on a vector containing a neomycin-resistance cassette. The promoter from the V18 gene has been shown to be active in vegetative cells and in starving cells up to 8-10 hours after the onset of starvation (20,23). The antisense vector was designed so that transformed cells will become resistant to neomycin and express antisense RNA complementary to the mRNA from which the cDNA was derived.…”
Section: Resultsmentioning
confidence: 99%
“…Approximately 25 % of the estimated 8000 genes in the Dictyostelium genome are regulated during the transition from unicellular to multicellular development [10]. So far, a large number of genes that are temporally regulated during development have been reported, and most of them participate in the progression of development [1,2,5,11]. However, the mechanism of transcriptional regulation of these genes remains to be solved.…”
mentioning
confidence: 99%