DNG1, a Dictyostelium homologue of tumor suppressor ING1 regulates differentiation of Dictyostelium cells

Mayanagi, Taira; Amagai, Aiko; Maeda, Yasuo

doi:10.1007/s00018-005-4570-0

Cited by 6 publications

(7 citation statements)

References 44 publications

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“…2000). Similarly, a recent report on DNG1, a Dictyostelium homologue of the tumor suppressor ING1, shows that dng1 ‐null cells express a much reduced level of csA/gp80 and form very small aggregates (Mayanagi et al. 2005).…”

Section: Regulation Of Temporal Expression Of Camsmentioning

confidence: 80%

Regulation of spatiotemporal expression of cell–cell adhesion molecules during development of Dictyostelium discoideum

Siu¹,

Sriskanthadevan²,

Wang³

et al. 2011

Dev Growth Differ

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The social amoeba Dictyostelium discoideum is a simple but powerful model organism for the study of cell-cell adhesion molecules and their role in morphogenesis during development. Three adhesive systems have been characterized and studied in detail. The spatiotemporal expression of these adhesion proteins is stringently regulated, often coinciding with major shifts in the morphological complexity of development. At the onset of development, amoeboid cells express the Ca 2+-dependent cell-cell adhesion molecule DdCAD-1, which initiates weak homophilic interactions between cells and assists in the recruitment of individuals into cell streams. DdCAD-1 is unique because it is synthesized as a soluble protein in the cytoplasm. It is targeted for presentation on the cell surface by an unconventional protein transport mechanism via the contractile vacuole. Concomitant with the aggregation stage is the expression of the contact sites A glycoprotein csA ⁄ gp80 and TgrC1, both of which mediate Ca 2+ ⁄ Mg 2+-independent cell-cell adhesion. Whereas csA ⁄ gp80 is a homophilic binding protein, TgrC1 binds to a heterophilic receptor on the cell. During cell aggregation, csA ⁄ gp80 associates preferentially with lipid rafts, which facilitate the rapid assembly of adhesion complexes. TgrC1 is synthesized at low levels during aggregation and rapid accumulation occurs initially in the peripheral cells of loose mounds. The extracellular portion of TgrC1 is shed and becomes part of the extracellular matrix. Additionally, analyses of knockout mutants have revealed important biological roles played by these adhesion proteins, including size regulation, cell sorting and cell-type proportioning.

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Section: Regulation Of Temporal Expression Of Camsmentioning

confidence: 80%

Regulation of spatiotemporal expression of cell–cell adhesion molecules during development of Dictyostelium discoideum

Siu¹,

Sriskanthadevan²,

Wang³

et al. 2011

Dev Growth Differ

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“…A recent commentary regarding the function of PHD fingers reiterated that the interaction of the PHD motif with methylated histone tails is not the primary mode of transcriptional regulation, but rather augments the interface of co-factors and chromatin remodeling complexes upon their recruitment to target promoters. (65) Chromatin remodeling via PHD fingers appears to be an evolutionarily conserved function, as ablation results in loss of histone modifications and improper transcriptional control from plants (76) to mammals. (77) Alterations to the chromatin modifying and transcriptional functions of PHD proteins are implicated in human diseases.…”

Section: Resultsmentioning

confidence: 99%

Pygopus and the Wnt signaling pathway: A diverse set of connections

Jessen

Dai

2008

BioEssays

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Identification of Pygopus in Drosophila as a dedicated component of the Wg (fly homolog of mammalian Wnt) signaling cascade initiated many inquiries into the mechanism of its function. Surprisingly, the nearly exclusive role for Pygopus in Wg signal transduction in flies is not seen in mice, where Pygopus appears to have both Wnt-related and Wnt-independent functions. This review addresses the initial findings of Pygopus as a Wg/Wnt co-activator in light of recent data from both fly and mammalian studies. We compare and contrast the developmental phenotypes of pygopus mutants to those characterized for known Wg/Wnt transducers and explore the data regarding a role for mammalian Pygopus 2 in tumorigenesis. We further analyze the roles of the two conserved domains of Pygopus proteins in transcription, and propose a model for the molecular mechanism of Pygopus function in both Wg/Wnt signaling and Wnt-independent transcriptional regulation.

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“…Also, the only other treatment that rescued the developmental delay itself was mixing wild-type cells with the tert KO cells at a 1:1 ratio (Fig 18; Table 2). Even though caffeine and glucose rescued streaming and mound size, and apparently this was at least partly mediated via their impact on cAMP-regulated processes, neither of the compounds rescued the delay, even though abnormalities of cAMP-regulated processes are commonly reported causes of delay in other Dictyostelium studies [52–54].…”

Section: Resultsmentioning

confidence: 99%

“…A line ending in an arrowhead suggests that the first element directly or indirectly promotes the activity or levels of the second; inhibition is suggested by a line ending in a cross-bar. Published works that report on the nature of each pathway within the network are as follows: a[29], [40]; b[29]; c[66]; d[52], [53], [54]; e[61], [82], [83], [84]; f[85]; g [86], [87]; h[88–90]; i[91], [33]; j[77]; k[92], [93], [94]; l[26], [39]; m[27]; n[35]; o[95], [96]; p[67]; q[67], [75]; r[67]; s[97]; t[75]; u[65]; v[98], [39]; w[99], [100]; x[66]; y[101].…”

Section: Introductionmentioning

confidence: 99%

A telomerase with novel non-canonical roles: TERT controls cellular aggregation and tissue size inDictyostelium

Nassir

Hyde

Baskar

2019

Preprint

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11Telomerase, particularly its main subunit, the reverse transcriptase, TERT, prevents DNA 12 erosion during eukaryotic chromosomal replication, but also has poorly understood non-13 canonical functions. Here, in the model social amoeba Dictyostelium discoideum, we show 14 that the protein encoded by tert has telomerase-like motifs and regulates, non-canonically, 15 important developmental processes. Expression levels of wild-type (WT) tert were biphasic, 16 peaking at 8 and 12 h post-starvation, aligning with developmental events, such as the 17 initiation of streaming (~7 h) and mound formation (~10 h). In tert KO mutants, however, 18 aggregation was delayed until 16 h. Large, irregular streams formed, then broke up, leading 19 to small mounds. The mound-size defect was not induced when a KO mutant of countin (a 20 master size-regulating gene) was treated with TERT inhibitors but anti-countin antibodies did 21 rescue size in the tert KO. Further, conditioned medium from countin mutants failed to 22 rescue size in the tert KO, but the converse experiment worked. These and additional 23 the migration of cells that form each fruiting body is delayed and irregular. These results are 48 significant because they show, for the first time, that a telomerase can influence cell 49 migration and tissue size regulation, two processes involved in a wide range of cancers. 50 51 Introduction 52 Each time a chromosome replicates, it loses some DNA from each of its ends. This is not 53 necessarily problematic, because the chromosome is initially capped at each end by a 54 sacrificial strand of non-coding DNA, a telomere [1][2][3]. Further instances of replication, 55 however, can expose the coding DNA, unless the cell can keep repairing the shortened 56 telomeres, by the action of the enzyme complex, telomerase. Accordingly, telomerase, whose 57 main subunits comprise a reverse transcriptase (TERT), and the telomerase RNA component 58 (TERC) [4], has much significance in the biology and pathology of multicellular organisms. 59As somatic tissues age, for example, telomerase is downregulated, and the resulting telomeric 60 dysfunction can lead to chromosomal instability and various pathologies, including disrupted 61 pregnancies and cancer [5][6][7]. In other cases, the upregulation of telomerase is also associated 62 with, and a biomarker of, some cancers, because it allows the unchecked proliferation of 63 immortalised tumour cells [6, 8]. Telomerase also has many non-canonical roles, in which 64 telomere maintenance, or even telomerase activity, is not required [9, 10]. For example, 65 telomerase is known to have non-canonical roles in neuronal differentiation [11], RNA 66 silencing [12], enhanced mitochondrial function [13] and various cancers [9, 14]. 67Our understanding of telomeres and telomerase began, and has continued to develop, through 68 the study of model organisms such as Drosophila, Zea mays, Tetrahymena, yeast and mice 69 [2, 3,[15][16][17][18][19]]. One model system in which the possible roles of telomerase hav...

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DNG1, a Dictyostelium homologue of tumor suppressor ING1 regulates differentiation of Dictyostelium cells

Cited by 6 publications

References 44 publications

Regulation of spatiotemporal expression of cell–cell adhesion molecules during development of Dictyostelium discoideum

Regulation of spatiotemporal expression of cell–cell adhesion molecules during development of Dictyostelium discoideum

Pygopus and the Wnt signaling pathway: A diverse set of connections

A telomerase with novel non-canonical roles: TERT controls cellular aggregation and tissue size inDictyostelium

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